IL-16 Antibody

IL-16 Antibody

Catalog# :4995

IL-16 was initially identified as a chemotactic cytokine, but is now known to possess a wide range of activities. Later studies have more fully characterized IL-16 as an immunomodulatory cytokine that contributes to the regulatory process of CD4+ T cell recruitment and activation at sites of inflammation in association with asthma and several autoimmune diseases. The precursor of IL-16 (pro-IL-16) is thought to be cleaved towards the C-terminal region by Caspase-3, releasing a 20 kDa active form that binds to and signals through CD4. Besides acting as a chemotactic cytokine, IL-16 is thought to also be involved in the regulation of T cell proliferation and multiple infectious, immune-mediated, and autoimmune inflammatory disorders including irritable bowel syndrome, systemic lupus erythematosus, and neurodegenerative disorders. At least two isoforms of IL-16 are known to exist; the longer isoform (also known as NIL-16) is detected only in neurons of the cerebellum and hippocampus. This antibody will only detect the NIL-16 isoform.

Additional Names : IL-16 (NT2), Interleukin 16, leukocyte chemoattractant factor, LCF, NIL-16
Description
Left: Western blot analysis of IL-16 in rat brain tissue lysate with IL-16 antibody at (A) 1 µg/ml and (B) 2 µg/ml.









Source :IL-16 antibody was raised against a 14 amino acid peptide near the amino terminus of human IL-16.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : IL-16 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies
Application : IL-16 antibody can be used for detection of IL-16 by Western blot at 1 – 2 µg/ml.
Tested Application(s) : E, WB
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide :Cat.No. 4995P - IL-16 Peptide
Long-Term Storage : IL-16 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control :
1. Cat. No. 1463 - Rat Brain Tissue Lysate
Species Reactivity : H, M, R
GI Number : 36953836
Accession Number : AAQ86961
Short Description : (NT2) Neuronal interleukin-16
References
1. Cruikshank WW, Center DM, Nisar N, et al. Molecular and functional analysis of a lymphocyte chemoattractant factor: association of biologic function with CD expression. Proc. Natl. Acad. Sci. USA 1994; 91:5109-13.
2. Interleukin-16. Cruikshank WW, Kornfeld H, and Center DM. J. Leukoc. Biol. 2000; 67:757-66.
3. Zhang Y, Center DM, Wu DM, et al. Processing and activation of pro-interleukin-16 by caspase-3. J. Biol. Chem. 1998; 273:1144-9.
4. Maciaszek JW, Parada NA, Cruikshank WW, et al. IL-16 represses HIV-1 promoter activity. J. Immunol. 1997; 158:5-8.

IL-16 Antibody

IL-16 Antibody

Catalog# :4985

IL-16 was initially identified as a chemotactic cytokine, but is now known to possess a wide range of activities. Later studies have more fully characterized IL-16 as an immunomodulatory cytokine that contributes to the regulatory process of CD4+ T cell recruitment and activation at sites of inflammation in association with asthma and several autoimmune diseases. The precursor of IL-16 (pro-IL-16) is thought to be cleaved towards the C-terminal region by Caspase-3, releasing a 20 kDa active form that binds to and signals through CD4. Besides acting as a chemotactic cytokine, IL-16 is thought to also be involved in the regulation of T cell proliferation and multiple infectious, immune-mediated, and autoimmune inflammatory disorders including irritable bowel syndrome, systemic lupus erythematosus, and neurodegenerative disorders. At least two isoforms of IL-16 are known to exist; the longer isoform (also known as NIL-16) is detected only in neurons of the cerebellum and hippocampus. This antibody will only detect the NIL-16 isoform.

Additional Names : IL-16 (NT), Interleukin 16, leukocyte chemoattractant factor, LCF, NIL-16
Description
Left: Western blot analysis of IL-16 in rat brain tissue lysate with IL-16 antibody at (A) 1 µg/ml and (B) 2 µg/ml.

Below:Immunohistochemistry of IL-16 in mouse brain tissue with IL-16 antibody at 2.5 µg/ml.

Other Product Images
Source :IL-16 antibody was raised against a 20 amino acid peptide near the amino terminus of human IL-16.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : IL-16 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application : IL-16 antibody can be used for detection of IL-16 by Western blot at 1 – 2 µg/ml.
Tested Application(s) : E, WB, IHC
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide :Cat.No. 4985P - IL-16 Peptide
Long-Term Storage : IL-16 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control :
1. Cat. No. 1463 - Rat Brain Tissue Lysate
Species Reactivity : H, M, R
GI Number : 36953836
Accession Number : AAQ86961
Short Description : (NT) Neuronal interleukin-16
References
1. Cruikshank WW, Center DM, Nisar N, et al. Molecular and functional analysis of a lymphocyte chemoattractant factor: association of biologic function with CD expression. Proc. Natl. Acad. Sci. USA 1994; 91:5109-13.
2. Interleukin-16. Cruikshank WW, Kornfeld H, and Center DM. J. Leukoc. Biol. 2000; 67:757-66.
3. Zhang Y, Center DM, Wu DM, et al. Processing and activation of pro-interleukin-16 by caspase-3. J. Biol. Chem. 1998; 273:1144-9.
4. Maciaszek JW, Parada NA, Cruikshank WW, et al. IL-16 represses HIV-1 promoter activity. J. Immunol. 1997; 158:5-8.

IFN-beta Antibody

IFN-beta Antibody

Catalog# :4243

Type I Interferons (IFN-a/b) are produced primarily in response to viral infection by “Natural IFN-producing cells" (IPCs) as part of the host immune response and can also inhibit the development of tumors. IFN-b binding by its receptor results in the activation of the tyrosine kinases Jak1 and Tyk2 and phosphorylation of members of the STAT family of transcription factors, leading to the transcription and expression of the immune response genes. More recently, several members of the toll-like receptor (TLR) family were found to stimulate the production IFN-b. IFN-b is currently used clinically for treatment of tumors, infections and multiple sclerosis.

Additional Names : IFN-beta (IN), Interferon beta, beta-interferon
Description
Left: Western blot analysis of IFN-b in A-20 cell lysate with IFN-b antibody at 5 µg/ml.

Below:Immunohistochemistry of IFN-b in human liver tissue with IFN-b antibody at 5 µg/ml.

Other Product Images

Source :IFN-beta antibody was raised against a 17 amino acid peptide from near the center of human IFN-b.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : IFN-beta antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application : IFN-b antibody can be used for detection of IFN-b by Western blot at ~5 µg/ml.
Tested Application(s) : E, WB, IHC
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide :Cat.No. 4243P - IFN-beta Peptide
Long-Term Storage : IFN-beta antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control :
1. Cat. No. 1288 - A20 Cell Lysate
2. Cat. No. 1304 - Human Liver Tissue Lysate
Species Reactivity : H, M
GI Number : 184623
Accession Number : AAC41702
Short Description : (IN) beta-interferon
References
1. Gresser I. Wherefore interferon? J. Leuk. Biol. 1997; 61:567-74.
2. Colonna M. TLR pathways and IFN-regulatory factors: to each its own. Eur. J. Immunol. 2007; 37:306-9.
3. Kock M, Mostert J, De Keyser J, et al. Interferon-beta treatment and the natural history of relapsing-remitting multiple sclerosis. Ann. Neurol. 2007; epub.

EBI3 Antibody

EBI3 Antibody

Catalog# :4977

EBI3 is a subunit in two distinct heterodimeric cytokines: interleukin-27 (IL-27) and IL-35 (1-3). Like interleukin-23 (IL-23), IL-27 is a recently discovered member of the IL-6/IL-12 family of proinflammatory and immunoregulatory cytokines. It exists as a heterodimer composed of the p40-related protein EBI3 and an IL-12 p35-related protein termed p28. IL-27 is produced after activation by antigen-presenting cells and induces proliferation of naïve but not memory CD4+ T-cells. It acts by binding to its receptor WSX-1 (also known as TCCR) and gp130 which results in the activation of a Jak/STAT signaling cascade, suggesting the IL-27 is involved in the regulation of immune processes. It has been suggested that IL-27 can also be used as a therapeutic agent against cancer as it can also induce tumor-specific anti-tumor activity mediated through CD8+ T-cells, IFN-g, and T-bet. IL-35 is composed of EBI3 and the p35 subunit of IL-12 and has been reported to have therapeutic effects against collagen-induced arthritis by expanding the population of regulatory T cells and suppressing Th17 cells. At least two isoform of EBI3 are known to exist.

Additional Names : EBI3 (IN), Epstein-Barr virus induced 3 precursor, interleukin-27 subunit beta, IL-27B
Description
Left: Western blot analysis of EBI3 in rat spleen tissue lysate with EBI3 antibody at 2 µg/ml.

Below:Immunohistochemistry of EBI3 in human spleen tissue with EBI3 antibody at 2.5 µg/ml.

Other Product Images
Source :
EBI3 antibody was raised against a 16 amino acid peptide from near the center of human EBI3.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : EBI3 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application : EBI3 antibody can be used for the detection of EBI3 by Western blot at 2 – 4 µg/ml.
Tested Application(s) : E, WB, IHC
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide :Cat.No. 4977P - EBI3 Peptide
Long-Term Storage : EBI3 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control :
1. Cat. No. 1466 - Rat Spleen Tissue Lysate
Species Reactivity : H, M, R
GI Number : 14577917
Accession Number : NP_005746
Short Description : (IN) Epstein-Barr virus induced 3 precursor
References
1. Pfanz S, Timans JC, Cheung J et al. IL-27, a heterodimeric cytokine composed of EBI3 and p28 protein, induces proliferation of naïve CD4(+) T cells. Immunity 2002; 16:779-90.
2. Devergne O, Birkenbach M, and Kieff E. Epstein-Barr virus-induced gene 3 and the p35 subunit of interleukin form a novel heterodimeric hematopoietin. Proc. Natl. Acad. Sci. USA 1997; 94:12041-6.
3. Niedbala W, Wei X, Cai B, et al. IL-35 is a novel cytokine with therapeutic effects against collagen-induced arthritis through the expression of regulatory T cells and suppression of Th17 cells. Eur. J. Immunol. 2007; 3021-29.
4. Pfanz S, Hibbert L, Mattson J, et al. WSX-1 and glycoprotein 130 constitute a signal-transducing receptor for IL-27. J. Immunol. 2004; 172:2225-31.

RNA Synthesis

RNA Synthesis

With over 20 years experience in custom synthesis for the biomedical research communities, BSI has developed the expertise to deliver custom synthesized RNA with quality that meets all your RNAi, siRNA, shRNA and other RNA projects.

Nucleotides

RNA, like DNA, is a polymer of nucleotides. Each nucleotide consists of a sugar that is attached to a phosphate group and any one of four bases. The RNA polymerase, as it builds the chain of nucleotides, processes only one of the two complementary strands of DNA. This DNA strand is referred to as the template strand. The least confusing name for the other DNA strand is "the nontemplate strand.

Common RNA

Before the nucleotides are linked together, they exist separately as ribonucleoside triphosphates (NTPs). As shown below, the NTPs contain one of the four common RNA bases, A, C, G, and U, linked to a five-carbon ribose sugar, linked, in turn, to a chain of three phosphate groups. During RNA synthesis, a covalent, "phosphodiester" bond is formed between one of the three phosphate groups on one NTP and a hydroxyl group on another. The two other phosphate groups that were part of the original NTP are released.

RNA Synthesis

RNA synthesis is said to proceed in the 5′ to 3′ direction, reflecting the fact that the attachment of new nucleotides always occurs at the 3′ hydroxyl group of the growing RNA chain. RNA synthesis goes through phases that are typical of polymerization processes: initiation, elongation, and termination, yielding an RNA product of defined size and sequence.

RNA Silencing

RNA Silencing

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Modified RNA

We are committed to long-term continuous improvement and developing the incorporation of modifications and minor bases.

RNA Conjugations

The BNA molecules can be chemically linked to a variety of other chemical groups. bioconjugates can be used in a variety of applications including but not limited to, Control of Gene Expression, Antisense Therapy, Cellular Uptake/Delivery, Drug/Effectors Delivery and many others.

RNA Chimeras

Synthetic molecules that can bind with high sequence specificity to a chosen target in a gene sequence are of major interest in medicinal and biotechnological contexts. They show promise for the development of gene therapeutic agents, diagnostic devices for genetic analysis, and as molecular tools for nucleic acid manipulations.

RNA Research Analysis

RNA Research Analysis

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Today RNA biology is becoming important in all areas of biomedical research. This is extremely useful for researchers who design molecules for RNA interference, and need to statistically test complex structure hypotheses.

RNA Catalysis

Research leading to the discovery that RNA can act as a catalyst started in the 1970’s. Thomas Cech, at the University of Colorado at Boulder, was studying the excision of introns in a ribosomal RNA gene in Tetrahymena thermophila.

RNA Processing

Post transcriptional modification is a genetic process in cell biology by which, in eukaryotic cells, primary transcript RNA is converted into mature RNA. It is most often associated with the conversion of precursor messenger RNA into mature messenger RNA (mRNA), which is also known as splicing, during the larger process of protein synthesis.

EBI3 Antibody

EBI3 Antibody

Catalog# :4967

EBI3 is a subunit in two distinct heterodimeric cytokines: interleukin-27 (IL-27) and IL-35 (1-3). Like interleukin-23 (IL-23), IL-27 is a recently discovered member of the IL-6/IL-12 family of proinflammatory and immunoregulatory cytokines. It exists as a heterodimer composed of the p40-related protein EBI3 and an IL-12 p35-related protein termed p28. IL-27 is produced after activation by antigen-presenting cells and induces proliferation of naïve but not memory CD4+ T-cells. It acts by binding to its receptor WSX-1 (also known as TCCR) and gp130 which results in the activation of a Jak/STAT signaling cascade, suggesting the IL-27 is involved in the regulation of immune processes. It has been suggested that IL-27 can also be used as a therapeutic agent against cancer as it can also induce tumor-specific anti-tumor activity mediated through CD8+ T-cells, IFN-g, and T-bet. IL-35 is composed of EBI3 and the p35 subunit of IL-12 and has been reported to have therapeutic effects against collagen-induced arthritis by expanding the population of regulatory T cells and suppressing Th17 cells. At least two isoform of EBI3 are known to exist.

Additional Names : EBI3 (CT), Epstein-Barr virus induced 3 precursor, interleukin-27 subunit beta, IL-27B
Description
Left: Western blot analysis of EBI3 in K562 lysate with EBI3 antibody at 1 µg/ml in (A) the absence and (B) the presence of blocking peptide.

Below:Immunohistochemistry of EBI3 in human spleen tissue with EBI3 antibody at 2.5 µg/ml.

Other Product Images

Source :EBI3 antibody was raised against a 16 amino acid peptide from near the carboxy terminus of human EBI3.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : EBI3 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application : EBI3 antibody can be used for the detection of EBI3 by Western blot at 1 – 2 µg/ml.
Tested Application(s) : E, WB, IHC
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide :Cat.No. 4967P - EBI3 Peptide
Long-Term Storage : EBI3 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control :
1. Cat. No. 1204 - K562 Cell Lysate
Species Reactivity : H, M
GI Number : 14577917
Accession Number : NP_005746
Short Description : (CT) Epstein-Barr virus induced 3 precursor
References

1. Pfanz S, Timans JC, Cheung J et al. IL-27, a heterodimeric cytokine composed of EBI3 and p28 protein, induces proliferation of naïve CD4(+) T cells. Immunity 2002; 16:779-90.
2. Devergne O, Birkenbach M, and Kieff E. Epstein-Barr virus-induced gene 3 and the p35 subunit of interleukin form a novel heterodimeric hematopoietin. Proc. Natl. Acad. Sci. USA 1997; 94:12041-6.
3. Niedbala W, Wei X, Cai B, et al. IL-35 is a novel cytokine with therapeutic effects against collagen-induced arthritis through the expression of regulatory T cells and suppression of Th17 cells. Eur. J. Immunol. 2007; 3021-29.
4. Pfanz S, Hibbert L, Mattson J, et al. WSX-1 and glycoprotein 130 constitute a signal-transducing receptor for IL-27. J. Immunol. 2004; 172:2225-31.

CRTH2 Antibody

CRTH2 Antibody

Catalog# :4029

The chemoattractant receptor-homologous molecule expressed on Th2 cells (CRTH2) is a recently identified receptor for the prostaglandin D2 (PGD2) in addition to the classic prostaglandin D receptor. CRTH2 is expressed on Th2 cells and eosinophils and mediates chemotaxis of these cells to PGD2 and is thus thought to be a key receptor mediating eosinophil and Th2 recruitment during allergic responses. However, CRTH2-null mice showed enhanced eosinophil recruitment into the lung consistent with observations that the CRTH2-null mice produced significantly higher amounts of interleukin-5 (IL-5) and IL-3. This suggests that CRTH2 plays a nonredundant role in restricting eosinophilia and allergic response in vivo. At least two different isoforms of CRTH2 are known to exist.

Additional Names : CRTH2 (IN), Chemoattractant receptor-homologous molecule expressed on Th2 cells, GPR44

Description
Left: Western blot analysis of CRTH2 in human heart tissue lysate with CRTH2 antibody at (A) 1 and (B) 2 µg/ml.

Below:Immunohistochemistry of CRTH2 in human heart tissue with CRTH2 antibody at 2.5 µg/ml.

Other Product Images
Source :CRTH2 antibody was raised against a 15 amino acid peptide from near the center of human CRTH2.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : CRTH2 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application : CRTH2 antibody can be used for detection of CRTH2 by Western blot at 2 µg/ml.
Tested Application(s) : E, WB, IHC
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide :Cat.No. 4029P - CRTH2 Peptide
Long-Term Storage : CRTH2 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control :
1. Cat. No. 1301 - Human Heart Tissue Lysate
Species Reactivity : H, M, R
GI Number : 153791424
Accession Number : NP_004769
Short Description : (IN) Chemoattractant receptor-homologous molecule expressed on Th2 cells
References
1. Nagata K, Hirai K, Tanaka K, et al. CRTH2, an orphan receptor of T-helper-2-cells, is expressed on basophils and eosinophils and responds to mast cell-derived factor(s). FEBS Lett. 1999; 459:195-9.
2. Shichijo M, Sugimoto H, Nagao K, et al. Chemoattractant receptor-homologous molecule expressed on Th2 cells activation in vivo increases blood leukocyte counts and its blockage abrogates 13,14-dihydro-15-keto-prostaglandin D2-induced eosinophilia in rats. J. Pharmacol. Exp. Ther. 2003; 307:518-25.
3. Chevalier E, Stock J, Fisher T, et al. Cutting edge: chemoattractant receptor-homologous molecule expressed on Th2 cells plays a restricting role on IL-5 production and eosinophil recruitment. J. Immunol. 2005; 2056-60. (07-01D)

CRTH2 Antibody

CRTH2 Antibody

Catalog# :4027

The chemoattractant receptor-homologous molecule expressed on Th2 cells (CRTH2) is a recently identified receptor for the prostaglandin D2 (PGD2) in addition to the classic prostaglandin D receptor. CRTH2 is expressed on Th2 cells and eosinophils and mediates chemotaxis of these cells to PGD2 and is thus thought to be a key receptor mediating eosinophil and Th2 recruitment during allergic responses. However, CRTH2-null mice showed enhanced eosinophil recruitment into the lung consistent with observations that the CRTH2-null mice produced significantly higher amounts of interleukin-5 (IL-5) and IL-3. This suggests that CRTH2 plays a nonredundant role in restricting eosinophilia and allergic response in vivo. At least two different isoforms of CRTH2 are known to exist.

Additional Names : CRTH2 (NT), Chemoattractant receptor-homologous molecule expressed on Th2 cells, GPR44
Description
Left: Western blot analysis of CRTH2 in Jurkat cell lysate with CRTH2 antibody at 1 µg/ml in (A) the absence and (B) presence of blocking peptide.

Below:Immunohistochemistry of CRTH2 in human heart tissue with CRTH2 antibody at 2.5 µg/ml.

Other Product Images
Source :CRTH2 antibody was raised against a 18 amino acid peptide from near the amino terminus of human CRTH2.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : CRTH2 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application : CRTH2 antibody can be used for detection of CRTH2 by Western blot at 0.5 – 1 µg/ml.
Tested Application(s) : E, WB, IHC
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide :Cat.No. 4027P - CRTH2 Peptide
Long-Term Storage : CRTH2 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
1. Cat. No. 1205 - Jurkat Cell Lysate
2. Cat. No. 1301 - Human Heart Tissue Lysate
Species Reactivity : H, M, R
GI Number : 153791424
Accession Number : NP_004769
Short Description : (NT) Chemoattractant receptor-homologous molecule expressed on Th2 cells
References
1. Nagata K, Hirai K, Tanaka K, et al. CRTH2, an orphan receptor of T-helper-2-cells, is expressed on basophils and eosinophils and responds to mast cell-derived factor(s). FEBS Lett. 1999; 459:195-9.
2. Shichijo M, Sugimoto H, Nagao K, et al. Chemoattractant receptor-homologous molecule expressed on Th2 cells activation in vivo increases blood leukocyte counts and its blockage abrogates 13,14-dihydro-15-keto-prostaglandin D2-induced eosinophilia in rats. J. Pharmacol. Exp. Ther. 2003; 307:518-25.
3. Chevalier E, Stock J, Fisher T, et al. Cutting edge: chemoattractant receptor-homologous molecule expressed on Th2 cells plays a restricting role on IL-5 production and eosinophil recruitment. J. Immunol. 2005; 2056-60. (07-01D)

CD4 Monoclonal Antibody

CD4 Monoclonal Antibody

Catalog# :PM-5203

CD4 is a member of the immunoglobulin superfamily and is implicated as associative recognition element in MHC (Major Histocompatibility Complex) class II-restricted immune response. On T-lymphocytes, it defines the helper/inducer subset. The mature 55 kd CD4 protein consists of a 372 amino acid extracellular segment composed of four tandem immunoglobulin-like VJ regions. The CD4 molecule is a major receptor for human immunodeficiency virus (HIV), binding directly to the envelope glycoprotein gp120 on HIV, with the co-receptors being CCR5 or CXCR4. It has been shown that the V-like domains are critical for binding with HIV envelope gp120.

Additional Names : CD4 (8G1B12), CD4 antigen, CD4 receptor
Description
Left: Western blot analysis of CD4 in rat lung tissue lysate with CD4 antibody at (A) 0.5 and (B) 1 µg/ml.










Source :Mouse monoclonal CD4 antibody was raised against a 193 amino acid recombinant protein from near the amino terminus of human CD4.
Purification : Immunoaffinity chromotography purified IgG
Clonality and Clone : This is a monoclonal antibody. (Clone 8G1B12)
Host : CD4 monoclonal antibody was raised in mouse.
Please use anti-mouse secondary antibodies.
Immunogen : Mouse monoclonal CD4 antibody was raised against a 193 amino acid recombinant protein from near the
Application : CD4 antibody can be used for detection of CD4 by Western blot at 0.5 – 1 µg/ml.
Tested Application(s) : E, WB
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide :Long-Term Storage : CD4 monoclonal antibody can be stored at 4ºC, stable for one year.
Positive Control :
1. Cat. No. 1462 - Rat Lung Tissue Lysate
Species Reactivity : H, M, R
Short Description : CD4 antigen
References
1. Bowers K, Pitcher C, and Marsh M. CD4 : a co-receptor in the immune response and HIV infection. Int. J. Biochem. Cell Biol. 1997; 29 :871-5.
2. Arthos J, Deen KC, Chaikin MA, et al. Identification of the residues in human CD4 critical for the binding of HIV. Cell 1989; 57:469-81.

RNA Protection Assays

RNA Protection Assays

Biosyn RNA Probe Synthesis are convenient, reliable, and cost-effective custom RNA product for ic-activity radioactive RNA probes for hybridization, RNase protection assays, and other applications. Our every RNA Synthesis Kit is designed by professional scientists. Biosyn offer custom RNA probe synthesis that is reliable and cost-effective. These high quality RNA probes can be use for hybridization, RNase protection assays, and other applications. The quality of our product is controlled in compliance of ISO 9001/2000 and delivered with HPLC profile and MALDI-TOF characterization.

RNA Probe

A nuclease protection assay is a laboratory test used in biochemistry and genetics to identify individual RNA molecules in a heterogeneous RNA sample extracted from cells. The technique can identify one or more RNA molecules of known sequence even at low total concentration.

Hybridization

In genetics, hybridization is the process of combining different varieties or species of organisms to create a hybrid (biology). In molecular biology, nucleic acid hybridization is the process of joining two complementary strands of DNA.

CD4 Monoclonal Antibody

CD4 Monoclonal Antibody

Catalog# :PM-5201

CD4 is a member of the immunoglobulin superfamily and is implicated as associative recognition element in MHC (Major Histocompatibility Complex) class II-restricted immune response. On T-lymphocytes, it defines the helper/inducer subset. The mature 55 kd CD4 protein consists of a 372 amino acid extracellular segment composed of four tandem immunoglobulin-like VJ regions. The CD4 molecule is a major receptor for human immunodeficiency virus (HIV), binding directly to the envelope glycoprotein gp120 on HIV, with the co-receptors being CCR5 or CXCR4. It has been shown that the V-like domains are critical for binding with HIV envelope gp120.

Additional Names : CD4 (9H5A8), CD4 antigen, CD4 receptor
Description
Left: Western blot analysis of CD4 in human thymus tissue lysate with CD4 antibody at (A) 0.5 and (B) 1 µg/ml.












Source :Mouse monoclonal CD4 antibody was raised against a 193 amino acid recombinant protein from near the amino terminus of human CD4.
Purification : Immunoaffinity chromotography purified IgG
Clonality and Clone : This is a monoclonal antibody. (Clone 9H5A8)
Host : CD4 monoclonal antibody was raised in mouse.
Please use anti-mouse secondary antibodies.
Immunogen : Mouse monoclonal CD4 antibody was raised against a 193 amino acid recombinant protein from near the
Application : CD4 antibody can be used for detection of CD4 by Western blot at 0.5 – 1 µg/ml.
Tested Application(s) : E, WB
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide :
Long-Term Storage : CD4 monoclonal antibody can be stored at 4ºC, stable for one year.
Positive Control :
1. Cat. No. 1314 - Human Thymus Tissue Lysate
Species Reactivity : H, M, R
Short Description : CD4 antigen
References
1. Bowers K, Pitcher C, and Marsh M. CD4 : a co-receptor in the immune response and HIV infection. Int. J. Biochem. Cell Biol. 1997; 29 :871-5.
2. Arthos J, Deen KC, Chaikin MA, et al. Identification of the residues in human CD4 critical for the binding of HIV. Cell 1989; 57:469-81.

Carabin Antibody

Carabin Antibody

Catalog# :4269

Antigen binding by the T-cell receptor (TCR) is one of the critical first steps in the immune response, triggering a cascade of signaling pathways that ultimately lead to T-cell activation. Screening a yeast two-hybrid screen of a human T-cell cDNA library with calcineurin, a protein phosphatase involved in multiple signaling pathways including T-cell activation, resulted in the identification of Carabin, a member of the TBC1 domain family of proteins, as a calcineurin-binding protein. Unlike other members of the TBC1 domain protein family which are thought to have a role in regulating cell growth and differentiation, further experiments demonstrated that Carabin is part of a negative regulatory loop for the intracellular TCR signaling pathway as well as an inhibitor of the Ras signaling pathway, suggesting that Carabin may also mediate crosstalk between calcineurin and Ras. Carabin antibody does not recognize TBC1D10A or TBC1D10B. Carabin is known to exist in multiple isoforms.

Additional Names : Carabin (NT), TBC1 domain family member 10C, TBC1D10C
Description
Left: Western blot analysis of Carabin in human spleen tissue lysate with Carabin antibody at (A) 1 and (B) 2 µg/ml.

Below:Immunohistochemistry of Carabin in human spleen tissue with Carabin antibody at 2.5 µg/ml.

Other Product Images
Source :Carabin antibody was raised against a 17 amino acid peptide from near the amino terminus of human Carabin.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : Carabin antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application : Carabin antibody can be used for detection of Carabin by Western blot at 1 – 2 µg/ml.
Tested Application(s) : E, WB, IHC
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide :Cat.No. 4269P - Carabin Peptide
Long-Term Storage : Carabin antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control :
1. Cat. No. 1306 - Human Spleen Tissue Lysate
Species Reactivity : H, M
GI Number : 38348348
Accession Number : NP_940919
Short Description : (NT) a negative regulator of the TCR signaling pathway
References
1. Weil R and Israel A. Deciphering the pathway from the TCR to NF-kappaB. Cell Death Differ. 2006; 13:826-33.
2. Im SH and Rao A. Activation and deactivation of gene expression Ca2+/calcineurin-NFAT-mediated signaling. Mol. Cells 2004; 18:1-9.
3. Pan F, Sun L, Kardian DB, et al. Feedback inhibition of calcineurin and ras by a dual inhibitory protein carabin. Nature 2007; 445:433-6.
4. White RA, Pasztor LM, Richardson PM, et al. The gene encoding TBC1D1 with homology to the tre-2/USP6 oncogene, Bub2, and cdc16 maps to mouse chromosome 5 and human chromosome 4. Cytogenet. Cell Genet. 2000; 89:272-5.

Carabin Antibody

Carabin Antibody

Catalog# :4267

Antigen binding by the T-cell receptor (TCR) is one of the critical first steps in the immune response, triggering a cascade of signaling pathways that ultimately lead to T-cell activation. Screening a yeast two-hybrid screen of a human T-cell cDNA library with calcineurin, a protein phosphatase involved in multiple signaling pathways including T-cell activation, resulted in the identification of Carabin, a member of the TBC1 domain family of proteins, as a calcineurin-binding protein. Unlike other members of the TBC1 domain protein family which are thought to have a role in regulating cell growth and differentiation, further experiments demonstrated that Carabin is part of a negative regulatory loop for the intracellular TCR signaling pathway as well as an inhibitor of the Ras signaling pathway, suggesting that Carabin may also mediate crosstalk between calcineurin and Ras. Carabin antibody does not recognize TBC1D10A or TBC1D10B. Carabin is known to exist in multiple isoforms.

Additional Names : Carabin (CT), TBC1 domain family member 10C, TBC1D10C

Description
Left: Western blot analysis of Carabin in Daudi cell lysate with Carabin antibody at (A) 1 and (B) 2 µg/ml.

Below:Immunohistochemistry of Carabin in human spleen tissue with Carabin antibody at 2.5 µg/ml.

Other Product Images
Source :Carabin antibody was raised against a 16 amino acid peptide from near the carboxy terminus of human Carabin.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : Carabin antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application : Carabin antibody can be used for detection of Carabin by Western blot at 1 – 2 µg/ml.
Tested Application(s) : E, WB, IHC
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide :Cat.No. 4267P - Carabin Peptide
Long-Term Storage : Carabin antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control :
1. Cat. No. 1224 - Daudi Cell Lysate
2. Cat. No. 1306 - Human Spleen Tissue Lysate
Species Reactivity : H, M
GI Number : 38348348
Accession Number : NP_940919
Short Description : (CT) a negative regulator of the TCR signaling pathway
References
1. Weil R and Israel A. Deciphering the pathway from the TCR to NF-kappaB. Cell Death Differ. 2006; 13:826-33.
2. Im SH and Rao A. Activation and deactivation of gene expression Ca2+/calcineurin-NFAT-mediated signaling. Mol. Cells 2004; 18:1-9.
3. Pan F, Sun L, Kardian DB, et al. Feedback inhibition of calcineurin and ras by a dual inhibitory protein carabin. Nature 2007; 445:433-6.
4. White RA, Pasztor LM, Richardson PM, et al. The gene encoding TBC1D1 with homology to the tre-2/USP6 oncogene, Bub2, and cdc16 maps to mouse chromosome 5 and human chromosome 4. Cytogenet. Cell Genet. 2000; 89:272-5.

Blimp-1 Antibody

Blimp-1 Antibody

Catalog# :3991

Blimp-1 was initially identified as a zinc finger-containing protein that drives the maturation of B lymphocytes into immunoglobulin-secreting cells (1). Together with X-box-binding protein 1 (XBP1), Blimp-1 is induced upon terminal differentiation of plasma cells (2,3). The transcriptional repressor activity of Blimp-1 has also been found to regulate T cell homeostasis and function, possibly by suppressing the expression of the cytokines IL-2 and interferon-gamma during T cell development (4). More recent experiments have suggested that Blimp-1 also plays a major role in the formation of primordial germ cells (PGC) in developing mammalian embryos. In these experiments, Blimp-1-deficient mutant mouse embryos form a cluster of PGC-like cells which fail to show the expected migration, proliferation, and repression of homeobox genes that normally accompany specification of primordial germ cells (5). Blimp-1 exists as at least two different isoforms.

Additional Names : Blimp-1 (NT), B-lymphocyte-induced maturation protein 1, PR domain-containing protein 1, PRDI-binding factor 1, PRDI-BF1
Description
Left: Western blot analysis of Blimp-1 in mouse lung tissue lysate with Blimp-1 antibody at (A) 0.5 and (B) 1µg/ml.

Below:Immunohistochemistry of Blimp-1 in mouse lung tissue with Blimp-1 antibody at 5 µg/ml.

Other Product Images
Source :Blimp-1 antibody was raised against a 17 amino acid peptide from near the amino terminus of human Blimp-1.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : Blimp-1 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application : Blimp-1 antibody can be used for detection of Blimp-1 by Western blot at 0.5 – 1 µg/ml.
Tested Application(s) : E, WB, IHC
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide :Cat.No. 3991P - Blimp-1 Peptide
Long-Term Storage : Blimp-1 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control :
1. Cat. No. 1402 - Mouse Lung Tissue Lysate
Species Reactivity : H, M, R
GI Number : 172072684
Accession Number : NP_001189
Short Description : (NT) B-lymphocyte-induced maturation protein 1
References
1. Angelin-Duclos C, Cattoretti G, Lin K-I, et al. Commitment of B lymphocytes to a plasma cell fate is associated with Blimp-1 expression. J. Immunol. 2000; 165:5462-71.
2. Reimold AM, Iwakoshi NN, Manis J, et al. Plasma cell differentiation requires the transcription factor XBP-1. Nature 2001; 412:300-7.
3. Martins GA, Cimmino L, Shapiro-Shelef M, et al. Transcriptional repressor Blimp-1 regulates T cell homeostasis and function. Nature Immunol. 2006; 7:457-65.

RNA Hybrid

RNA Hybrid

RNA hybrid in biosynthesis is a double-stranded polynucleic acid in which one strand is DNA and the other strand is the complementary RNA; formed during transcription and during multiplication of oncogenic RNA viruses. One of several forms that can be assumed by a double helix. A-DNA is stable in dehydrated conditions.

Double-Stranded Polynucleic Acids

A nuclease (a nucleate oligonucleotidohydrolase) that forms oligonucleotides ending in 5'-phosphates from RNA and DNA; hydrolyzes both double-stranded and single-stranded polynucleic acids. This form is less common than the dominant form found under physiological conditions -- beta-dna.

RNA Hybrid

RNA hybrid is a double-stranded polynucleic acids in which one strand is DNA and the other strand is the complementary RNA; formed during transcription and during multiplication of oncogenic RNA viruses.This form is also assumed by DNA-RNA hybrid helices and by regions of double-stranded RNA. It is a right-handed helix and is a more compact form than beta-DNA.

Blimp-1 Antibody

Blimp-1 Antibody

Catalog# :3989

Blimp-1 was initially identified as a zinc finger-containing protein that drives the maturation of B lymphocytes into immunoglobulin-secreting cells. Together with X-box-binding protein 1 (XBP1), Blimp-1 is induced upon terminal differentiation of plasma cells. The transcriptional repressor activity of Blimp-1 has also been found to regulate T cell homeostasis and function, possibly by suppressing the expression of the cytokines IL-2 and interferon-gamma during T cell development. More recent experiments have suggested that Blimp-1 also plays a major role in the formation of primordial germ cells (PGC) in developing mammalian embryos. In these experiments, Blimp-1-deficient mutant mouse embryos form a cluster of PGC-like cells which fail to show the expected migration, proliferation, and repression of homeobox genes that normally accompany specification of primordial germ cells.

Additional Names : Blimp-1 (CT), Presenilin enhancer protein 2, Gamma-secretase subunit PEN-2
Description
Left: Western blot analysis of Blimp-1 in A549 cell lysate with Blimp-1 antibody at (A) 0.5 and (B) 1µg/ml.

Below: Immunofluorescence of Blimp-1 in mouse lung tissue with Blimp-1 antibody at 20 µg/ml.

Other Product Images
Source :PEN2 antibody was raised against a 13 amino acid peptide from near the carboxy terminus of human PEN2.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : Blimp-1 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application : Blimp-1 antibody can be used for detection of Blimp-1 by Western blot at 0.5 – 1 µg/ml.
Tested Application(s) : E, WB, IF
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide :Cat.No. 3989P - Blimp-1 Peptide
Long-Term Storage : Blimp-1 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control :
1. Cat. No. 1203 - A549 Cell Lysate
Species Reactivity : H, M, R
GI Number : 172072684
Accession Number : NP_001189
Short Description : (CT) B-lymphocyte-induced maturation protein 1
References
1. Turner CAJ, Mack DH, and Davis MM. Blimp-1, a novel zinc finger-containing protein that can drive the maturation of B-lymphocytes into immunoglobulin-secreting cells. Cell 1994; 77:297-306.
2. Angelin-Duclos C, Cattoretti G, Lin K-I, et al. Commitment of B lymphocytes to a plasma cell fate is associated with Blimp-1 expression. J. Immunol. 2000; 165:5462-71.
3. Reimold AM, Iwakoshi NN, Manis J, et al. Plasma cell differentiation requires the transcription factor XBP-1. Nature 2001; 412:300-7.
4. Martins GA, Cimmino L, Shapiro-Shelef M, et al. Transcriptional repressor Blimp-1 regulates T cell homeostasis and function. Nature Immunol. 2006; 7:457-65.

ADAP Antibody

ADAP Antibody

Catalog# :4279

The adhesion and degranulation adaptor protein (ADAP) was initially identified as a molecular adapter that couples T cell receptor (TCR) stimulation to the avidity of integrins governing T cell adhesion. TCR stimulation promotes the formation of a multi-protein complex containing CARMA1, MALT1, and BCL-10, which through the association of ADAP, ultimately activates the NF-kappaB family of transcription factors. More recent experiments have shown that ADAP controls optimal T cell proliferation, cytokine production, and expression of the Bcl-2 family member Bcl-x(L), suggesting that ADAP regulates T cell activation by promoting antigen-dependent T cell-antigen presenting cell (APC) activation. At least three isoforms of ADAP are known to exist.

Additional Names : ADAP (NT), Adhesion and degranulation adapter protein, FYN binding protein, FYB-120/130, SLAP-130
Description
Left: Western blot analysis of ADAP in K562 cell lysate with ADAP antibody at (A) 0.5 and (B) 1 µg/ml.

Below: Immunocytochemistry of ADAP in K562 cells with ADAP antibody at 10 µg/ml.

Other Product Images
Source :ADAP antibody was raised against a 17 amino acid peptide from near the amino terminus of human ADAP.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : ADAP antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application : ADAP antibody can be used for detection of ADAP by Western blot at 0.5 – 1 µg/ml.
Tested Application(s) : E, WB, ICC
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide :Cat.No. 4279P - ADAP Peptide
Long-Term Storage : ADAP antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control :
1. Cat. No. 1204 - K562 Cell Lysate
Species Reactivity : H
GI Number : 42476118
Accession Number : NP_001456
Short Description : (NT) Adhesion and degranulation adapter protein
References
1. Griffiths EK, Krawczyk C, Kong YY, et al. Positive regulation of T cell activation and integrin adhesion by the adapter Fyb/Slap. Science 2001; 293:2260-3.
2. Rawlings DJ, Sommer K, and Moreno-Garcia ME. The CARMA1 signalosome links the signalling machinery of adaptive and innate immunity in lymphocytes. Nat. Rev. Immunol. 2006; 6:799-812.
3. Medeiros RB, Burbach BJ, Mueller KL, et al. Regulation of NF-kappaB activation in T cells via association of the adapter proteins ADAP and CARMA1. Science 2007; 316:754-8.
4. Mueller KL, Thomas MS, Burbach BJ, et al. Adhesion and degranulation-promoting adapter protein (ADAP) positively regulates T cell sensitivity to antigen and T cell survival. J. Immunol. 2007; 179:3559-69.

ADAP Antibody

ADAP Antibody

Catalog# :4279

The adhesion and degranulation adaptor protein (ADAP) was initially identified as a molecular adapter that couples T cell receptor (TCR) stimulation to the avidity of integrins governing T cell adhesion. TCR stimulation promotes the formation of a multi-protein complex containing CARMA1, MALT1, and BCL-10, which through the association of ADAP, ultimately activates the NF-kappaB family of transcription factors. More recent experiments have shown that ADAP controls optimal T cell proliferation, cytokine production, and expression of the Bcl-2 family member Bcl-x(L), suggesting that ADAP regulates T cell activation by promoting antigen-dependent T cell-antigen presenting cell (APC) activation. At least three isoforms of ADAP are known to exist.

Additional Names : ADAP (NT), Adhesion and degranulation adapter protein, FYN binding protein, FYB-120/130, SLAP-130

Description
Left: Western blot analysis of ADAP in K562 cell lysate with ADAP antibody at (A) 0.5 and (B) 1 µg/ml.

Below: Immunocytochemistry of ADAP in K562 cells with ADAP antibody at 10 µg/ml.

Other Product Images
Source :ADAP antibody was raised against a 17 amino acid peptide from near the amino terminus of human ADAP.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : ADAP antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application : ADAP antibody can be used for detection of ADAP by Western blot at 0.5 – 1 µg/ml.
Tested Application(s) : E, WB, ICC
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide :Cat.No. 4279P - ADAP Peptide
Long-Term Storage : ADAP can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control :
1. Cat. No. 1204 - K562 Cell Lysate
Species Reactivity : H
GI Number : 42476118
Accession Number : NP_001456
Short Description : (NT) Adhesion and degranulation adapter protein
References
1. Griffiths EK, Krawczyk C, Kong YY, et al. Positive regulation of T cell activation and integrin adhesion by the adapter Fyb/Slap. Science 2001; 293:2260-3.
2. Rawlings DJ, Sommer K, and Moreno-Garcia ME. The CARMA1 signalosome links the signalling machinery of adaptive and innate immunity in lymphocytes. Nat. Rev. Immunol. 2006; 6:799-812.
3. Medeiros RB, Burbach BJ, Mueller KL, et al. Regulation of NF-kappaB activation in T cells via association of the adapter proteins ADAP and CARMA1. Science 2007; 316:754-8.
4. Mueller KL, Thomas MS, Burbach BJ, et al. Adhesion and degranulation-promoting adapter protein (ADAP) positively regulates T cell sensitivity to antigen and T cell survival. J. Immunol. 2007; 179:3559-69.

ADAP Antibody

ADAP Antibody

Catalog# :4277

The adhesion and degranulation adaptor protein (ADAP) was initially identified as a molecular adapter that couples T cell receptor (TCR) stimulation to the avidity of integrins governing T cell adhesion. TCR stimulation promotes the formation of a multi-protein complex containing CARMA1, MALT1, and BCL-10, which through the association of ADAP, ultimately activates the NF-kappaB family of transcription factors. More recent experiments have shown that ADAP controls optimal T cell proliferation, cytokine production, and expression of the Bcl-2 family member Bcl-x(L), suggesting that ADAP regulates T cell activation by promoting antigen-dependent T cell-antigen presenting cell (APC) activation. At least three isoforms of ADAP are known to exist.

Additional Names : ADAP (CT), Adhesion and degranulation adapter protein, FYN binding protein, FYB-120/130, SLAP-130
Description
Left: Western blot analysis of ADAP in K562 cell lysate with ADAP antibody at (A) 0.5 and (B) 1 µg/ml.

Below: Immunocytochemistry of ADAP in K562 cells with ADAP antibody at 10 µg/ml.

Other Product Images
Source :ADAP antibody was raised against a 20 amino acid peptide from near the carboxy terminus of human ADAP.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : ADAP antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application : ADAP antibody can be used for detection of ADAP by Western blot at 0.5 – 1 µg/ml.
Tested Application(s) : E, WB, ICC
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide :Cat.No. 4277P - ADAP Peptide
Long-Term Storage : ADAP antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control :
1. Cat. No. 1204 - K562 Cell Lysate
Species Reactivity : H, M
GI Number : 42476118
Accession Number : NP_001456
Short Description : (CT) Adhesion and degranulation adapter protein
References
1. Griffiths EK, Krawczyk C, Kong YY, et al. Positive regulation of T cell activation and integrin adhesion by the adapter Fyb/Slap. Science 2001; 293:2260-3.
2. Rawlings DJ, Sommer K, and Moreno-Garcia ME. The CARMA1 signalosome links the signalling machinery of adaptive and innate immunity in lymphocytes. Nat. Rev. Immunol. 2006; 6:799-812.
3. Medeiros RB, Burbach BJ, Mueller KL, et al. Regulation of NF-kappaB activation in T cells via association of the adapter proteins ADAP and CARMA1. Science 2007; 316:754-8.
4. Mueller KL, Thomas MS, Burbach BJ, et al. Adhesion and degranulation-promoting adapter protein (ADAP) positively regulates T cell sensitivity to antigen and T cell survival. J. Immunol. 2007; 179:3559-69.

RNA Chimeras Synthesis

Biosyn experience in to BNA/DNA chimera synthesis allows us to offer the synthesis of a chimera at competitive prices and quick delivery. Our every RNA Chimeras synthesis is handled by professional scientists.

RNA Oligos

RNA is shipped deprotected and desalted in 2-3 business days or deprotected and purified in 4-6 business days. Please inquire for turnaround on 5 µmole and 10 µmole RNA synthesis. RNA oligos are provided with sodium as the counter-ion. Through the powerful science of RNA interference, successful gene silencing can be achieved with the use of synthetic, small interfering RNA (siRNA).

RNA Replication

It is therefore likely that the RNA replication reactions of these two picornaviruses involve an equivalent set of protein-protein and protein-RNA interactions and perhaps utilize the same host cell proteins. By targeting small, synthetic oligos, typically 19-23 bases in length against a specific gene of interest researchers are identifying gene function, elucidating pathways, and screening for potential new drug targets.

RNA Chimera

RNA Chimera

Biosyn experience in to BNA/DNA chimera synthesis allows us to offer the synthesis of a chimera at competitive prices and quick delivery. Our every RNA Chimera tests are designed by professional scientists.

RNA Oligos

RNA is shipped deprotected and desalted in 2-3 business days or deprotected and purified in 4-6 business days. Please inquire for turnaround on 5 µmole and 10 µmole RNA synthesis. RNA oligos are provided with sodium as the counter-ion. Through the powerful science of RNA interference, successful gene silencing can be achieved with the use of synthetic, small interfering RNA (siRNA).

RNA Replication

It is therefore likely that the RNA replication reactions of these two picornaviruses involve an equivalent set of protein-protein and protein-RNA interactions and perhaps utilize the same host cell proteins. By targeting small, synthetic oligos, typically 19-23 bases in length against a specific gene of interest researchers are identifying gene function, elucidating pathways, and screening for potential new drug targets.

Constrained Nucleotide Nucleic Acid

Constrained Nucleotide Nucleic Acid

A Constrained Nucleotide, often referred to as inaccessible RNA, is a modified RNA nucleotide. The ribose moiety of an Constrained Nucleotide nucleotide is modified with an extra bridge connecting the 2' and 4' carbons. The bridge "locks" the ribose in the 3'-endo structural conformation, which is often found in the A-form of DNA or RNA.

Aminoallyl nucleotide

Aminoallyl nucleotides are used in post-labeling of nucleic acids to be used in microarrays. These nucleotides are formally known as 5-(3-aminoallyl)-nucleotides since the aminoallyl group is usually attached to carbon 5 of the pyrimidine ring of uracil and cytosine. They are usually abbreviated as aa-, such as aa-dUTP.

Oligonucleotide

An Oligonucleotide is a short segment of RNA or DNA, typically with twenty or fewer bases. Although they can be formed by cleavage of longer segments, they are now more commonly synthesized by polymerizing individual nucleotide precursors. Automated synthesizers allow the synthesis of oligonucleotides up to 160 to 200 bases.

ZIP10 Antibody

ZIP10 Antibody

Catalog# :4991

ZIP10, also known as Slc39A10, is a widely expressed zinc transporter with nine transmembrane domains. Zinc is an essential ion for cells and plays significant roles in the growth, development, and differentiation. ZIP10 mRNA was found to be significantly decreased in the intestines and kidneys of hypothyroid rats and increased in those of hyperthyroid rats, indicating that ZIP10 is positively regulated by thyroid hormones. ZIP10 mRNA was also found to be upregulated in invasive and metastatic breast cancer and cell lines, suggesting that ZIP10 could serve as a possible marker for the metastatic phenotype and possibly a target for novel treatment strategies. At least three isoforms of ZIP10 are known to exist. This antibody will not cross-react with the zinc transporter ZIP11.

Additional Names : ZIP10, Solute carrier family 39 member a10, Slc39a10, LZT-Hs2
Description
Left:Western blot analysis of ZIP10 in human spleen tissue lysate with ZIP10 antibody at (A) 1 and (B) 2 µg/ml.

Below Immunohistochemistry of ZIP10 in human spleen tissue with ZIP10 antibody at 2.5 µg/ml.

Other Product Images

Source :ZIP10 antibody was raised against a 17 amino acid peptide near the center of the human ZIP10.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : ZIP10 antibody was raised in rabbit.
Application : ZIP10 antibody can be used for detection of ZIP10 by Western blot at 1 – 2 µg/ml.
Tested Application(s) : E, WB, IHC
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide :Cat.No. 4991P - ZIP10 Peptide
Long-Term Storage : ZIP10 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control :
1. Cat. No. 1306 - Human Spleen Tissue Lysate
Species Reactivity : H, M, R
GI Number : 149046180
Accession Number : EDL99073
Short Description : Solute carrier family 39 member a10
References
1. Kaler P and Prasad R. Molecular cloning and functional characterization of novel transporter rZip10 (Slc39a10) involved in zinc uptake across renal brush-border membrane. Am. J. Renal Physiol. 2007; 292:F217-29.
2. Taylor KM and Nicohlson RI. The LZT proteins; the LIV-1 subfamily of zinc transporters. Biochim. Biophys. Acta. 2003; 1611:16-30.
3. Pawan K, Neeraj S, Sandeep K, et al. Upregulation of Slc39a10 gene expression in response to thyroid hormones in intestine and kidney. Biochim. Biophys. Acta. 2007; 1769:117-23.
4. Kagara N, Tanaka N, Noguchi S, et al. Zinc and its transporter ZIP10 are involved in invasive behavior of breast cancer cells. Cancer Sci. 2007; 98:692-7.