Catalog# : 4543
Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. Two of the strongest hits implicate genes IRGM and ATG16L1, which encode proteins thought to be critical to the autophagy pathway and being significantly associated with Crohn’s disease. In mouse, IRGM belongs to a family of gamma-interferon-induced GTP-binding proteins of approximately 48 kDa. Murine IRGM induces autophagy and generates large autolysosomal organelles as a mechanism for the elimination of intracellular Mycobacterium tuberculosis. Human IRGM is also involved in autophagy and plays a role in the control of intracellular pathogens and in the reduction of intracellular bacillary load.
Additional Names : IRGM (NT), Immunity related GTPase family, IFL1, IRGM1, LRG47, LRG-47
DescriptionLeft: Western blot analysis of IRGM in Rat brain lysate with IRGM antibody at (A) 1 and (B) 2 µg/ml.
Source : IRGM antibody was raised against a 17 amino acid peptide near the amino terminus of the human IRGM.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : IRGM antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.
Application : IRGM antibody can be used for detection of IRGM by Western blot at 1 – 2 µg/ml.
Tested Application(s) : E, WB
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide : Cat.No. 4543P - IRGM Peptide
Long-Term Storage : IRGM antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
1. Cat. No. 1463 - Rat Brain Tissue Lysate
Species Reactivity :H, M, R
GI Number : 118764009
Accession Number : AAI28169
Short Description : (NT) gamma-interferon-induced GTP-binding protein
References
1. Gozuacik D and Kimchi A. Autophagy as a cell death and tumor suppressor mechanism. Oncogene 2004; 23:2891-906.
2. Massey DC and Parkes M. Genome-wide association scanning highlights two autophagy genes, ATG16L1 and IRGM, as being significantly associated with Crohn's disease. Autophagy 2007; 3:649-51.
3. Fisher SA, Tremelling M, Anderson CA, et al. Genetic determinants of ulcerative colitis include the ECM1 locus and five loci implicated in Crohn's disease. Nat. Genet. 2008; 40:710-2.
4. Singh SB, Davis AS, Taylor GA, et al. Human IRGM induces autophagy to eliminate intracellular mycobacteria. Science 2006; 313:1438-41.
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