Protein Carrier Conjugation

Protein Carrier Conjugation

Bio-Synthesis offers conjugation of peptides to a variety of immunogenic carrier proteins and gel for use as immunogen or for affinity purification. A variety of immunogenic carrier proteins such as Keyhole Limpet Hemocyanin (KLH), Diphtheria toxoid has been use in conjugation with several conjugation chemistries.We also offer coupling of peptide to various types of gel to create an immunosorbent, for positive or negative selection of macromolecular binding entries, such as antibodies.

Services:

Carrier
Keyhole Limpet Hemocyanin (KLH), Bovine Serum Albumin (BSA), Rabbit Serum Albumin (RSA), Ovalbumin, Thyroglobulin, or Human Gamma Globulin (HGG). Other proteins are available upon request.

Cross-Links
MBS (and other bi-functional linkers), which couple through sulfhydryl groups, and glutaraldehyde, which couples primarily through alpha, sigma & epsilon amino groups

Amino Acid Analysis of Conjugates available upon request

References:
[1] Lee, A.C., Powell, J.E., Tregear, G.W., Niall, H.D. and Stevens, V.C. (1980) A method for preparing b-hCG COOH peptide-carrier conjugates of predictable composition. Mol. Immunol. 17; 749-756.