Catalog# :2345
Telomerase is an RNA-dependent DNA polymerase that uses an RNA component to add telomeric repeat sequences at the ends of chromosomes. Besides the RNA component which serves as the template that specifies the telomeric repeat, the telomerase complex contains a reverse transcriptase protein (TRT) and various accessory proteins including the telomerase-associated protein 1 (TP1). Telomerase activity is low in most somatic cells, causing the gradual shortening of telomeres which can ultimately lead to telomere fusion and cell death. High levels of telomerase activity are widely seen in cancerous cells and while recent experiments have suggested that telomerase may be a viable target in cancer therapy, expression levels of TP1 do not correlate with malignancy. At least two isoforms of TP1 are known to exist.
Additional Names : TP1, Telomerase-associated protein 1, telomerase protein 1, TEP1
Description
Left: Western blot analysis of TP1 in human kidney tissue lysate with TP1 antibody at (A) 1 and (B) 2 µg/ml.
Below Immunohistochemical staining of human lung tissue using TP1 antibody at 2.5 µg/ml.
Other Product Images
Source :TP1 antibody was raised against a 20 amino acid peptide from near the amino terminus of human TP1.Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : TP1 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies
Application : TP1 antibody can be used for detection of TP1 by Western blot at 1 – 2 µg/ml.
Tested Application(s) : E, WB, IHC
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide :Cat.No. 2345P - TP1 Peptide
Long-Term Storage : TP1 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control :
1. Cat. No. 1305 - Human Kidney Tissue Lysate
Species Reactivity : H, M, R
GI Number : 1848277
Accession Number : AAC51107
Short Description : Telomerase-associated protein 1
References
1. Greider CW. Telomere length regulation. Annu. Rev. Biochem. 1996; 274:92-7.
2. Cong YS, Wright WE, and Shay JW. Human telomerase and its regulation. Microbiol. Mol. Biol. Rev. 2002; 66:407-25.
3. Harrington L, McPhail T, Mar V, et al. A mammalian telomerase-associated protein. Science 1997; 275:973-7.
4. Wong JMY and Collins K. Telomere maintenance and disease. Lancet 2003; 362:983-8.
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