The leucine-rich, glioma inactivated gene 2 (LGI2) is a member of the LGI family in which LGI1 is the exemplar. The LGI family consists of four of highly related proteins containing leucine-rich repeats (LRRs) which are highly similar to other transmembrane signaling molecules and receptors. LGI1 has been identified as a candidate tumor suppressor gene for glioma and plays a role in autodominant lateral temporal epilepsy (ADTLE), an epileptic syndrome characterized by focal seizures with predominant auditory symptoms. Despite its high homology with LGI1 and similar pattern of expression, mutations in LGI2 have not been found to be associated with ADTLE. Three isoforms of LGI2 are known to exist; this LGI2 antibody will recognize only the longest. This LGI2 antibody is predicted to be specific to LGI2 and not recognize other LGI proteins.
Additional Names : LGI2 (IN), Leucine-rich, glioma inactivated 2, LGIL2
Description
Left: Western blot analysis of LGI2 in mouse liver tissue lysate with LGI2 antibody at (A) 1 and (B) 2 µg/ml.
Below: Immunohistochemical staining of human brain tissue using LGI2 antibody at 2.5 µg/ml.
Other Product Images
Source :LGI2 antibody was raised against a 10 amino acid peptide from near the center of human LGI2.Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : LGI2 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application : LGI2 antibody can be used for the detection of LGI2 by Western blot at 1 – 2 µg/ml.
Tested Application(s) : E, WB, IHC
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide :Cat.No. 4533P - LGI2 Peptide
Long-Term Storage : LGI2 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control :
1. Cat. No. 1404 - Mouse Liver Tissue Lysate
Species Reactivity : H, M
GI Number : 32469737
Accession Number : Q8K4Z0
Short Description : (IN) Leucine-rich, glioma inactivated 2
References
1. Gu W, Gibert Y, Wirth T, et al. Using gene-history and expression analysis to assess the involvement of LGI genes in human disorders. Mol. Biol. Evol. 2005; 22:2209-16.
2. Chernova OB, Somerville RP and Cowell JK. A novel gene, LGI1, from 10q24 is rearranged and downregulated in malignant brain tumors. Oncogene 1998; 17:2873-81.
3. Berkovic SF, Izzillo P, McMahon JM, et al. LGI1 mutations in temporal lobe epilepsies. Neurology 2004; 62:1115-9.
No comments:
Post a Comment