Catalog#:3553
Adipose tissue of an organism plays a major role in regulating physiologic and pathologic processes such as metabolism and immunity by producing and secreting a variety of bioactive molecules termed adipokines (reviewed in 1). One highly conserved family of adipokines is adiponectin/ACRP30 and its structural and functional paralogs, the C1q/tumor necrosis factor-?-related proteins (CTRPs) 1-7 (2). Unlike the CTRPs, which are expressed in a wide variety of tissues, adiponectin is reported to be expressed exclusively by differentiated adipocytes (3). These proteins are thought to act mainly on liver and muscle tissue to control glucose and lipid metabolism. An analysis of the crystal structure of adiponectin revealed a structural and evolutionary link between TNF and C1q-containing proteins, suggesting that these proteins arose from a common ancestral innate immunity gene (4). It is present in high levels in normal human plasma, but is reduced in obese subjects and often in those with increased insulin resistance and type 2 diabetes, suggesting that adiponectin may be a useful pharmacological target in various metabolic diseases (5).
Additional Names: Adiponectin (NT), ACRP30, adipocyte complement-related protein 30
Description
Left: Western blot analysis of adiponectin in rat brain cell lysate with adiponectin antibody at (A) 1, (B) 2, and (C) 4 µg/ml.
Below: Immunohistochemistry of adiponectin in rat brain tissue with adiponectin antibody at 1 µg/ml.
Source:Adiponectin antibody was raised against a 15 amino acid peptide from near the amino terminus of human adiponectin.
Purification: Affinity chromatography purified via peptide column
Clonality and Clone: This is a polyclonal antibody.
Host: Adiponectin antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen: Human Adiponectin / ACRP30 / adipocyte complement-related protein 30 (N-Terminus) Peptide (Cat. No.
Application: Adiponectin antibody can be used for the detection of adiponectin by Western blot at 1 µg/ml.
Tested Application(s): E, WB, IHC
Buffer: Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide:Cat. No. 3553P - Adiponectin Peptide
Long-Term Storage: Adiponectin antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control:
1. Cat. No. 1463 - Rat Brain Tissue Lysate
Species Reactivity: H, M, R
GI Number: 4757760
Accession Number: NP_004788
Short Description: (NT) A novel adipokine
References
1. Fantuzzi G. Adipose tissue, adipokines, and inflammation. J. Allergy Clin. Immunol. 2005; 115:911-9.
2. Tsao T-S, Lodish HF, and Fruebis J. ACRP30, a new hormone controlling fat and glucose metabolism. Euro. J. Pharmacol. 2002; 440:213-21.
3. Wong GW, Wang J, Hug C, et al. A family of Acrp30/ adiponectin structural and functional paralogs. Proc. Natl. Acad. Sci. USA 2004; 101:10302-7.
4. Shapiro L and Scherer PE. The crystal structure of a complement-1q family protein suggests an evolutionary link to tumor necrosis factor. Curr. Biol. 1998; 8:335-8.
1 comment:
Adipose tissue of an organism plays a major role in regulating physiologic and pathologic processes such as metabolism and immunity by producing and secreting a variety of bioactive molecules termed adipokines. It is a reagent originally manufactured or offered by bbgent. Thanks a lot...
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