Catalog#:3681
Disruptions of protein folding and maturation in the endoplasmic reticulum (ER) result in the activation of the unfolded protein response (UPR), an integrated cellular signaling pathway that transmits information from the ER lumen to the cytoplasm and nucleus. Activating transcription factor 6 (ATF6) as well as the ER-transmembrane protein kinases IRE1p and PERK are the major transducers of the UPR . ATF6 is an ER transmembrane protein that is normally bound to the ER chaperone GRP78, but upon ER stress is released from GRP78 and proteolytically cleaved to yield a cytosolic fragment which then migrates to the nucleus, and together with the transcription factor XBP-1, activates transcription of UPR-responsive genes (2-4). ATF6 has two isoforms (ATF6Α and ATF6Β); only ATF6Α is recognized by this antibody.
Additional Names: ATF6 (CT), Activating transcription factor 6
Description
Left: Western blot analysis of ATF6 in MCF7 cell lysate with ATF6 antibody at (A) 0.5 and (B) 1 μg/ml.
Below: Immunocytochemical staining of MCF7 cells using ATF6 antibody at 10 μg/ml.
Other Product Images
Source:ATF6 antibody was raised against a 16 amino acid peptide from near the carboxy terminus of human ATF6.
Purification: Affinity chromatography purified via peptide column
Clonality and Clone: This is a polyclonal antibody.
Host: ATF6 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen: Human ATF6 (C-Terminus) Peptide (Cat. No. 3681P)
Application: ATF6 antibody can be used for the detection of ATF6 by Western blot at 0.5 - 1 µg/ml.
Tested Application(s): E, WB, ICC
Buffer: Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide:Cat. No. 3681P - ATF6 Peptide
Long-Term Storage: ATF6 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control:
1. Cat. No. 1219 - MCF7 Cell Lysate
Species Reactivity: H, M
GI Number: 56786157
Accession Number: NP_031374
Short Description: (CT) An ER signaling protein of the unfolded protein response pathway
References
1. Liu CY and Kaufman RJ. The unfolded protein response. J. Cell Sci. 2003; 1861-2.
2. Haze K, Yoshida H, Yanagi H, et al. Mammalian transcription factor ATF6 is synthesized as a transmembrane protein and activated by proteolysis in response to endoplasmic stress. Mol. Cell. Biol. 1999; 10:3787-99.
3. Little E, Ramakrishnan M, Roy B, et al. The glucose-regulated proteins (GRP78 and GRP94): functions, gene regulation, and applications. Crit. Rev. Eukaryot. Gene Expr. 1994; 4:1-18.
4. Yoshida H, Matsui T, Yamamoto T, et al. XBP1 mRNA is induced by ATF6 and spliced by IRE1p in response to ER stress to produce a highly active transcription factor. Cell 2001; 107:881-91.
1 comment:
Hello,
ATF6 is a constitutively expressed, endoplasmic reticulum membrane-anchored transcription factor. It is a key transcriptional activator of the unfolded protein response, which allows mammalian cells to maintain cellular homeostasis when they are subjected to environmental and physiological stresses that target the ER. Thanks...
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