Showing posts with label Custom Peptide Antibody Production. Show all posts
Showing posts with label Custom Peptide Antibody Production. Show all posts

Wednesday, June 22, 2011

Clone Antibody



Bio-Synthesis, with 20 years of experience in developing polyclonal antibodies, is a leading global provider of custom antibody production services. Our facility generates high-affinity custom antibodies with industry-leading titer guarantees and comprehensive antibody production packages that simplify the ordering process and present cost-effective methods to isolate epitope-specific antibodies. Visit www.biosyn.com and see why partner with Bio-Synthesis for your custom antibodies needs.

Antibodies

From Antigen selection to antibody purification, BSI has optimized each step of the antibody production process to provide the best antibodies available.

Custom peptide antibody production

Keyhole Limpet Hemocyanin (KLH), Bovine Serum Albumin (BSA), Rabbit Serum Albumin (RSA), Ovalbumin, Thyroglobulin, or Human Gamma Globulin (HGG).Amino Acid Analysis of Conjugates also available.

Isotypes

Antibodies can come in different varieties known as isotypes or classes. In placental mammals there are five antibody isotypes known as IgA, IgD, IgE,IgG and IgM.The antibody isotype of a B cell changes during the cell's development and activation.









For more information visit: www.biosyn.com

Sunday, September 26, 2010

Protein Carrier Conjugation


Bio-Synthesis offers conjugation of peptides to a variety of immunogenic carrier proteins, and gel, for use as an immunogen or for affinity purification. A variety of immunogenic carrier proteins such as Keyhole Limpet Hemocyanin (KLH) and Diphtheria toxoid have been use in conjugation with several conjugation chemistries. We also offer coupling of peptides to various types of gel to create an immunosorbent, for positive or negative selection of macromolecular binding entries, such as antibodies.

Serum production packages also available with large selection of host animals, click here to explore more on our custom peptide antibody production.

Carrier: Keyhole Limpet Hemocyanin (KLH), Bovine Serum Albumin (BSA), Rabbit Serum Albumin (RSA), Ovalbumin, Thyroglobulin, or Human Gamma Globulin (HGG). Other proteins are available upon request.

Cross-Links: MBS (and other bi-functional linkers), which couple through sulfhydryl groups, and glutaraldehyde, which couples primarily through alpha, sigma & epsilon amino groups


* Amino Acid Analysis of Conjugates available upon request


References:
[1] Lee, A.C., Powell, J.E., Tregear, G.W., Niall, H.D. and Stevens, V.C. (1980) A method for preparing b-hCG COOH peptide-carrier conjugates of predictable composition. Mol. Immunol. 17; 749-756.

[2] Posnett, D., McGrath, H., and Tam, J.P. A novel method for producing anti-peptide antibodies
, The Journal of Biological Chemistry, 263, 1719-1725 (1988),

[3] Tam, J.P., Synthetic peptide vaccine design: synthesis and properties of a high-density multiple antigenic peptide system, Proc. Natl. Acad, Sci., 85, 5409-5413 (1988).

[4] Wang, C. Y., Looney, D.J., Li, M.L., Walfield, A.M., Ye, J., Hosein, B., Tam, J.P. and Wong-Staal, F., Long-term high-titer neutralizing activity induced by octameric synthetic HIV antigen, Science, 254, 285-288. (1991)