LNA-DNA Chimeras in Cancer Treatments

Oncologists have been searching for a breakthrough in cancer research and medicine for a longtime now. Although a “cure” to cancer is still leaps and bounds out of reach, oncologists found the KRAS gene to which to turn their attention and target. It was found that a cancer with the wild type form of the KRAS gene responds better to EGFR inhibiting cancer drugs than cancers with a mutation in this gene. It is not a huge step towards ultimately preventing or reversing cancer, but it is important towards the practical treatment of cancer.

Oncologists had thus started using a technique involving LNA-DNA chimeras in order to detect and determine whether or not a particular person’s cancer cells contain any mutated form of the KRAS gene. Using LNA-DNA chimeras in order to block the amplification of wild type KRAS alleles, PCR is performed to amplify any possible trace amounts of existing mutated KRAS alleles. It was found that this simple and low-cost technique, called wild-type blocking PCR (WTB-PCR), lead to a higher success rate of detecting mutated alleles, which lead to more increased efficiency in cancer treatment.

LNA chimeras are becoming used much more widely and as possible uses are discovered for these nucleotides with high stability, research based around LNA will increase greatly.

Custom DNA Synthesis

BSI offers DNA base synthesis in phosphodiester, phosphorothioate and methylphosphonate backbones. We offer an extensive selection of purifications and oligo modifications, such as linkers, spacers, dyes and modified bases. Also available:

* Custom oligonucleotide preparative and analytical services
* Long oligonucleotide synthesis for gene expression
* Antisense oligonucleotide synthesis
* Large scale ASR oligonucleotide productions

As always, every custom oligonucleotide synthesis is: All of our custom oligonucleotide products are:

Deprotected, desalted, lyophilized, Ready-2-Use
Quantitated 2x by UV spectrophotometry to provide an accurate measure of yield
QC checked by mass spectrometry
Fast delivery with high throughput capacity

Standard DNA (2' Deoxy) Oligonucleotide Synthesis

Unmodified Standard DNA Purifications


Unmodified Standard DNA Purifications Yields