DNA-Binding Proteins

DNA-Binding Proteins

Bio-Synthesis, founded in 1984, is an international provider of genomic services established around the core business lines oligonucleotide and gene synthesis. Base, ribose and backbone modifications and reporter groups like dyes and haptens are routinely incorporated in DNA/RNA hybrid synthesis. Through innovative production process and synthesis know-how, our services are the best in their class, providing you with innovative and trustworthy solutions to accelerate your research.

DNA Binding Proteins

DNA-binding proteins include transcription factors which modulate the process of transcription, nucleases which cleave DNA molecules, and histones which are involved in DNA packaging in the cell nucleus. DNA-binding proteins can incorporate such DNA-binding domains as the zinc finger, the helix-turn-helix, and the leucine zipper (among many others).

Single Strand Binding Protein

Single-strand binding protein, also known as SSB or SSBP, binds single stranded regions of DNA to prevent premature reannealing. The strands have a natural tendency to revert to the duplex form, but SSB binds to the single strands, keeping them separate and allowing the DNA replication machinery to perform its function.

Protein

A protein is a complex, high molecular weight organic compound that consists of amino acids joined by peptide bonds. Organize articles about proteins according to a standard protein ontology.

Labelling Proteins

Labelling Proteins

Bio-Synthesis offers a complete array of fluorescent reporters and quenchers. Our product offereing includes common dye modifiers such as 6-FAM, HEX and TET, as well as exotic modifictions. When labeling protein/peptide are needed, cross-linking two molecules by using a specific functional group such as an amine, thiol modified oligonucleotide with an certain cross linkers are employed.

Protein Synthesis

The process in which individual amino acids, whether of exogenous or endogenous origin, are connected to each other in peptide linkage in a specific order dictated by the sequence of nucleotides in DNA; this governing sequence is conveyed to the synthesizing apparatus in the ribosomes by mRNA, formed by base-pairing on the DNA template.

DNA Binding Proteins

Structural proteins that bind DNA are well-understood examples of non-specific DNA-protein interactions. Within chromosomes, DNA is held in complexes with structural proteins. These proteins organize the DNA into a compact structure called chromatin. In eukaryotes this structure involves DNA binding to a complex of small basic proteins called his tones, while in prokaryotes multiple types of proteins are involved.

Topoisomerases and Helicases

Topoisomerases are enzymes with both nuclease and ligase activity. These proteins change the amount of supercoiling in DNA. Some of these enzyme work by cutting the DNA helix and allowing one section to rotate, thereby reducing its level of super coiling; the enzyme then seals the DNA break.