Oligo Nucleotide

Oligo Nucleotide

For over 20 years, BioSynthesis has provided custom oligonucleotide production services worldwide, including researcher at university, biotechnology and pharmaceutical institutions. We are not only synthesizing standard DNA primer and probes but are also specialized in production and purification of modified oligomers. Base, ribose and backbone modifications and reporter groups like dyes and haptens are routinely incorporated at small discovery research scale to multi-gram ASR/cGMP for clinical diagnostic production. As always, quality is guaranteed!

Oligonucleotide

An oligonucleotide (or oligo) is a short segment of RNA or DNA, typically with twenty or fewer bases. Although they can be formed by cleavage of longer segments, they are now more commonly synthesized by polymerizing individual nucleotide precursors. Automated synthesizers allow the synthesis of oligonucleotides up to 160 to 200 bases. The length of the oligonucleotide is usually denoted by "mer" (from Greek meros, "part"). For example, a fragment of 25 bases would be called a 25-mer. Oligonucleotides are often used as probes for detecting DNA or RNA because they bind readily to their complements.

Synthesis

Oligonucleotides are chemically synthesized using nucleotides, called phosphoramidites, normal nucleotides that have protection groups: preventing amine, hydroxyl groups and phosphate groups interacting incorrectly. One phosphoramidite is added at the time, the product's 5' phosphate is deprotected, and a new base is added, and so on (backwards); at the end, all the protection groups are removed.

Oligonucleotide Synthesis

Oligonucleotide synthesis is the non-biological, chemical synthesis of defined short sequences of nucleic acids. It is extremely useful in laboratory procedures covering a wide range of molecular biology applications. Automated synthesizers allow the synthesis of oligonucleotides up to 160 to 200 bases. Typically, synthesized oligonucleotides are single-stranded DNA molecules around 15-20 bases in length.!

DNA/Constrained Nucleotide Oligonucleotides Hybridized

DNA/Constrained Nucleotide Oligonucleotides Hybridized

Bio-Synthesis - the world leader in custom services since 1984.With the industry growing so rapidly, there are "young" companies sprouting all over the place. Many of these same companies are not successful and do not last very long. Bio-Synthesis has been the world leader in custom services since our inception in 1984. Over two decades of the highest quality of products, coupled with fast turnaround time and affordability has enabled BSI to stand in no one else's shadow.

Constrained Nucleotide Oligonucleotide

Custom Constrained Nucleotide Oligonucleotide synthesis High quality Custom Constrained Nucleotide Oligonucleotides are available for a variety of different specialty applications and innovative products.

Oligonucleotide Synthesis

It is the non-biological, chemical synthesis of defined short sequences of nucleic acids. It is extremely useful in laboratory procedures covering a wide range of molecular biology applications. Automated synthesizers allow the synthesis of oligonucleotides up to 160 to 200 bases.

Modified Oligos

Modified oligos terminated with the 5'-Thiol-Modifier C6 is shipped with the trityl protecting group still attached to the sulfur. In order to free the thiol group for use removes the trityl group and reduces the sulfur. Oligonucleotides terminated with the 5'-Thiol-Modifier C6 SS (Disulfide) will need to be reduced before use.

Double Stranded Oligo

Double Stranded Oligo

Since 1984, Bio-Synthesis, the first company to provide commercial custom oligonucleotide synthesis services, has remained a major force in advancing biotechnology research both as leading supplier and developer of new technologies for oligo synthesis. Combining unique know-how, constantly evolving technology and wide experience in oligo manufacturing and services, BSI expanded its oligo synthesis from custom DNA, RNA, Constrained Nucleotide BNA (bridged nucleic acid) synthesis with SPEED and QUALITY.

Oligonucleotide Synthesis

Oligonucleotide synthesis is the non-biological, chemical synthesis of defined short sequences of nucleic acids. It is extremely useful in laboratory procedures covering a wide range of molecular biology applications. Automated synthesizers allow the synthesis of oligonucleotides up to 160 to 200 bases. Typically, synthesized oligonucleotides are single-stranded DNA molecules around 15-20 bases in length.

Synthesis Substrates

Oligonucleotides are chemically synthesized using phosphoramidites. A phosphoramidite is a normal nucleotide with protection groups added to its reactive amine, hydroxyl and phosphate groups. These protection groups prevent unwanted side reactions and force the formation of the desired product during synthesis. The 5' hydroxyl group is protected by DMT (dimethoxytrityl), the phosphate group by a diisopropylamino (iPr2N) group and a 2-cyanoethyl (OCH2CH2CN) group. The bases also have protecting groups on the exocyclic amine group (benzoyl or isobutyryl).

Sequential Synthesis

Whereas enzymes synthesize DNA in a 5' to 3' direction, chemical DNA synthesis is done backwards in a 3' to 5' reaction. Based on the desired nucleotide sequence of the product, the phosphoramidites for the bases A, C, G, and T are added sequentially to react with the growing chain in a repeating cycle until the sequence is complete. In each cycle, the product's 5' phosphate is deprotected and a new base is added for extension.

RNA Cell

RNA Cell

Biosynthesis can provide RNA, RNA analogs and chimeras in large quantities for your diagnostic and therapeutic research. Biosyn continues the tradition of providing researchers with the highest quality RNA Oligonucleotide synthesis, in quantities ranging from multi-mg to several grams. The quality of our product is controlled in compliance of ISO 9001/2000 and delivered with HPLC profile and MALDI-TOF characterization.

RNA Molecules

RNA molecules play critical and diverse roles in a staggering number of cellular processes. Processes of particular interest in the Department of Cell Biology include the examination of how RNA molecules fold within cells, how RNA-binding proteins recognize their RNA targets, how mRNAs are localized to discrete regions of cells and how small noncoding RNAs influence gene expression and cell function.

RNA Oligonucleotide Synthesis

Oligonucleotide synthesis is the non-biological, chemical synthesis of defined short sequences of nucleic acids. It is extremely useful in laboratory procedures covering a wide range of molecular biology applications. Automated synthesizers allow the synthesis of oligonucleotides up to 200 bases even longer.

RNA Research Analysis

Today RNA biology is becoming important in all areas of biomedical research. This is extremely useful for researchers who design molecules for RNA interference, and need to statistically test complex structure hypotheses.

For More information Visit : www.biosyn.com or call at :972-420-8505 !Now!

Oligonucleotide synthesis

Oligonucleotide synthesis is the non-biological, chemical synthesis of defined short sequences of nucleic acids. It is extremely useful in laboratory procedures covering a wide range of molecular biology applications. Automated synthesizers allow the synthesis of oligonucleotides up to 160 to 200 bases. Typically, synthesized oligonucleotides are single-stranded DNA molecules around 15-20 bases in length. They are most commonly used as primers for DNA sequencing and amplification, as probes for detecting complementary DNA or RNA via molecular hybridization, and for the targeted introduction of mutations and restriction sites, allowing for the synthesis of artificial genes.

Synthesis substrates

Oligonucleotides are chemically synthesized using phosphoramidites. A phosphoramidite is a normal nucleotide with protection groups added to its reactive amine, hydroxyl and phosphate groups. These protection groups prevent unwanted side reactions and force the formation of the desired product during synthesis. The 5' hydroxyl group is protected by DMT (dimethoxytrityl), the phosphate group by a diisopropylamino (iPr2N) group and a 2-cyanoethyl (OCH2CH2CN) group.

Sequential synthesis

In solid-phase synthesis, the 3' end of the oligonucleotide is bound to a solid support column on which all reactions take place. The 3' group of the first base is immobilized via a linker onto a solid support (polystyrene beads or similar). This allows for easy addition and removal of reactants. In each step, the solutions with the nucleotides for the next reaction are pumped through the column from an attached reagent delivery system and washed out before the next nucleotide is added. In modern synthesizers, reagent delivery and washing steps are controlled via computer based on the desired sequence. At the end of the synthesis program, the oligonucleotide is cleaved off the solid support and eluted from the column.


Microarrays

An interesting development of this technology has allowed gene chips to be made, where the probes are synthesized on the silicon chip, and not printed, allowing a higher resolution. This can be done via a mechanical mask where thin silicon rubber capillaries are put on a glass slide and the probes synthesized. More high-tech versions employ photolayable products and Photolithographic mask or micro mirrors. The 1cm2 surface of silicon is coated with a linker and a photo protecting group such as nitroveratryloxycarbonyl is used and the mask exposes to a lamp the spots that will receive the subsequent nucleotide: this step is repeated for all four bases, but only one correct one is added to the growing probes on each spot