Catalog# : NB-28111
Price/Unit : $155.00
Unit : 100 reactions
M.W. g/mol
2X SYBR Green/Fluorescein qPCR Master Mix
- Convenient ready-to-use solution
- High sensitivity and yield
- Robust amplification
- Available in 3 convenient sizes: 100, 200 and 500 reactions (20 µL vol)
BSI’s 2X SYBR Green/Fluorescein qPCR Master Mix is a ready-to-use solution that contains components required for quantitative PCR amplification with Bio-Rad iCycler series of real time PCR systems (iQ®, MyiQTM, iQ5TM). The mix includes Taq DNA polymerase, dNTPs, reaction buffer, MgCl2, KCl, a PCR enhancer/stabilizer, SYBR Green and Fluorescein. The user needs only to add template, the primer set and water to the Master Mix to set up the qPCR reaction. This convenient 2X SYBR Green/Fluorescein qPCR Master Mix reduces the time required to set up qPCR reactions and reduces the possibility of contamination particularly when preparing large numbers of reactions. Fluorescein was included in the mix allowing dynamic well factors collection for Bio-Rad iCycler series (iQ®, MyiQTM, iQ5TM) machines. The optimized master mix produces a reliable and sensitive result for specific quantification of target DNA or cDNA from genomic, plasmid and viral templates.
Taq DNA Polymerase is a highly thermostable DNA polymerase that possesses a 5´→ 3´ polymerase activity and a very low 5´→ 3´ exonuclease activity. The source of Taq included with BSI’s 2X SYBR Green/Fluorescein qPCR Master Mix is an E. coli strain with a cloned Taq DNA Polymerase gene from Thermus aquaticus YT-1.
BSI’s 2X SYBR Green/Fluorescein qPCR Master Mix is available in 3 convenient sizes:
Cat # 28111 - 100 reactions (sufficient for 100 reactions x 20 µL reaction volume)
Cat # 28109 - 200 reactions (sufficient for 200 reactions x 20 µL reaction volume)
Cat # 28110 – 500 reactions (sufficient for 500 reactions x 20 µL reaction volume)
FEATURES AND BENEFITS
- Convenient - With the ready-to-use Master Mix, the user needs only to add template, the primer set and water to the master mix in order to set up the qPCR reaction.
- Time Savings - Set up qPCR reactions in a shorter time, as less pipetting steps are required.
- Cost Efficient - No need to buy separate enzymes, dNTPs and buffers. All are included with the ready-to-use Master Mix.
- High Sensitivity and yield – The optimized Master Mix allows for highly sensitive amplifications with high yields of qPCR products.
- Robust Amplification - BSI’s 2X SYBR Green/Fluorescein qPCR Master Mix is capable of amplifying difficult templates with a high degree of reproducibility.
- Routine quantitative PCR amplification of DNA templates or cDNA templates
- qPCR for post reverse transcription step
Figure 1. Uniform and Reproducible Results. BSI’s 2X SYBR Green/Fluorescein qPCR Master Mix was used to amplify a 10-fold dilution series (5 ng–500 fg) of Staphylococcus sp. genomic DNA using primers specific to NADPH-1 gene. Duplicate reactions at each concentration were amplified on the iCycler iQ real-time system. From observing the graph above it can be seen that BSI’s Master Mix provided uniform and reproducible results at all the various template concentrations.SPECIFICATIONS
Reagents supplied – Cat# 28111
2X SYBR Green/Fluorescein qPCR Master Mix (1 Vials, 100 Reactions) - Sufficient reagent for 100 x 20 µL reactions
Reagents supplied – Cat# 28109
2X SYBR Green/Fluorescein qPCR Master Mix (2 Vials, 100 Reactions each) - Sufficient reagent for 200 x 20 µL reactions
Reagents supplied – Cat# 28110
2X SYBR Green/Fluorescein qPCR Master Mix (5 Vials, 100 Reactions each) - Sufficient reagent for 500 x 20 µL reactions
Storage Conditions and Product Stability
BSI’s 2X SYBR Green/Fluorescein qPCR Master Mix should be stored at -20°C. For everyday use an aliquot can be stored at 4°C for up to 3 months. Repeated freeze-thaw cycles are not recommended. When stored at the proper temperature this reagent is stable for at least 1 year.
Long-Term Storage : Norgen’s 2X SYBR Green/Fluorescein qPCR Master Mix should be stored at -20°C. For everyday use an aliquot can be stored at 4°C for up to 3 months. Repeated freeze-thaw cycles are not recommended. When stored at the proper temperature this reagent is stable for at least 1 year.
Product Usage
For laboratory research use only, not for diagnostic use! Not for use in humans.
For more detail regarding Peptide please visit here : Custom Peptide Synthesis
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