CARD10 Antibody

CARD10 Antibody

Catalog# : 3013

Apoptosis is related to many diseases and development. Cell death signals are transduced by death domain (DD), death effector domain (DED), and caspase recruitment domain (CARD) containing molecules. CARD containing proteins include some caspases, Apaf-1, CARD4, IAPs, RICK, ARC, RAIDD, Bcl-10, and ASC. A novel CARD-containing protein was recently identified and designated CARD10 (1). This protein belongs to the membrane-associated guanylate kinase-like (MAGUK) family of proteins that can function as molecular scaffolds that assist assembly of signal transduction molecules (2). CARD 10 interacts with Bcl-10, a Bcl protein that promotes apoptosis, caspase-9 maturation and activation of NF-kappaB (1,3). CARD 10 and the related protein CARMA1 also associate with NEMO, the regulatory subunit of the IkappaK complex (4), demonstrating its importance in the regulation of NF-kappaB transcription factor activation.

Additional Names : CARD10, Caspase recuitment domain family 10 / CARMA3 / Bimp1

Description
Left: Western blot analysis of CARD10 expression in EL4 cell lysate with CARD10 antibody at (A) 5 and (B) 10 µg /ml.

Below: Immunocytochemistry of CARD10 in EL4 cells with CARD10 antibody at 10 µg/ml.

Other Product Images


Source : CARD10 antibody was raised against a synthetic peptide corresponding to 14 amino acids at the C-terminus of human CARD10.

Purification : immunoaffinity purified

Clonality and Clone : This is a polyclonal antibody.

Host : CARD10 antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.

Immunogen : Murine CARD10 / Caspase recuitment domain family 10 / CARMA3 / Bimp1 Peptide (Cat. No. 3013P)

Application : CARD10 antibody can be used for detection of CARD10 by Western blot at 5 - 10 µg/ml.

Tested Application(s) : E, WB, ICC

Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.

Blocking Peptide : Cat. No. 3013P - CARD10 Peptide

Long-Term Storage : CARD10 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Positive Control
1.Cat. No. 1287 - EL4 Cell Lysate

Species Reactivity :H, M

GI Number : 13488607

Accession Number : AAK26165

Short Description : Caspase recuitment domain family 10

References
1.Wang L, Guo Y, Huang W-J, et al. CARD10 is a novel caspase recruitment domain/membrane-associated guanylate kinase family member that interacts with BCL10 and activates NF-κB. J. Biol. Chem. 2001; 276:21405-9.
2.Fanning AS and Anderson JM. Protein modules as organizers of membrane structure. Curr. Opin. Cell Biol. 1999; 11:432-9.
3.Fischer KD, Tedford K, Wirth T. New roles for Bcl10 in B-cell development and LPS response. Trends Immunol. 2004; 25:113-6.
4.Stilo R, Liguoro D, Di Jeso B, et al. Physical and functional interaction of CARMA1 and CARMA3 with Iκ kinase κ-NF-κB essential modulator. J. Biol. Chem. 2004; 279:34323-31.

For more detail regarding Peptide please visit here : Custom Peptide Synthesis