DRAM Antibody

DRAM Antibody

Catalog# : 4035

Damage-regulated autophagy modulator (DRAM) is a p53 target gene encoding a lysosomal protein that induces autophagy, a process that degrades cytosolic proteins and organelles. It has been suggested that activation of DRAM by p53 is simultaneous to the activation by p53 of one or more proapoptotic genes such as PUMA, Bax, etc., and that the signaling pathways regulated by these genes together promote a full cell death response. By itself, DRAM cannot induce apoptosis, but the fact that it is inactivated in certain cancers highlights the importance of DRAM and suggests that autophagy may play a more important role in cancer than initially suspected. At least two different isoforms of DRAM are known to exist.

Additional Names : DRAM (NT), Damage-regulated autophagy modulator

Description
Left: Western blot analysis of DRAM in K562 cell lysate with DRAM antibody at (A) 0.5, (B) 1 and (C) 2 µg/ml.

Below: Immunohistochemistry of DRAM in human liver tissue with DRAM antibody at 2.5 µg/ml.

Other Product Images
Source : DRAM antibody was raised against a 16 amino acid peptide from near the amino terminus of human DRAM.

Purification : Affinity chromatography purified via peptide column

Clonality and Clone : This is a polyclonal antibody.

Host : DRAM antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.

Application : DRAM antibody can be used for detection of DRAM by Western blot at 0.5 – 1 µg/ml.

Tested Application(s) : E, WB, IHC

Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.

Blocking Peptide : Cat.No. 4035P - DRAM Peptide

Long-Term Storage : DRAM antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Positive Control
1.Cat. No. 1204 - K562 Cell Lysate
2.Cat. No. 1304 - Human Liver Tissue Lysate

Species Reactivity :H, M, R

GI Number : 22450862

Accession Number : AAH18435

Short Description : (NT) Damage-regulated autophagy modulator

References
1.Crighton D, Wilkinson S, O’Prey J, et al. DRAM, a p53-induced modulator of autophagy, is critical for apoptosis. Cell 2006; 126:121-34.
2.Gozuacik D and Kimchi A. Autophagy as a cell death and tumor suppressor mechanism. Oncogene. 2004; 23:2891-906.
3.Crighton D, Wilkinson S, and Ryan KM. DRAM links autophagy to p53 and programmed cell death. Autophagy 2007; 3:72-4. (07-01D)