GNPDA2 Antibody

GNPDA2 Antibody

Catalog# : 5061

Glucosamine-6-phosphate deaminase (GNPDA) is an allosteric enzyme that catalyzes the reversible conversion of D-glucosamine-6-phosphate into D-fructose-6-phosphate and ammonium. GNPDA2 is the second mammalian glucosamine-6-phosphate deaminase discovered, and is closer in structure and activity to the E. coli enzyme than GNPDA1. GNPDA1 possesses greater affinity for ammonium than either GNPDA2 or the E. coli enzyme suggesting that the forward reaction of D-glucosamine-6-phosphate into D-fructose-6-phosphate and ammonium is catalyzed at a slower rate than GNPDA2. GNPDA2 antibody is predicted to not cross-react with GNPDA1.
Additional Names : GNPDA2, Glucosamine-6-phosphate deaminase 2, GNPI

Description
Left: Western blot analysis of GNPDA2 in human kidney lysate with GNPDA2 antibody at 1 µg/ml.

Below: Immunocytochemistry of GNPDA2 in 293 cells with GNPDA2 antibody at 4 µg/ml.

Other Product Images
Source : GNPDA2 antibody was raised against an 8 amino acid peptide near the carboxy terminus of human GNPDA2.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : GNPDA2 antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.
Application : GNPDA2 antibody can be used for detection of GNPDA2 by Western blot at 1 – 2 µg/ml.
Tested Application(s) : E, WB, ICC
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide : Cat.No. 5061P - GNPDA2 Peptide
Long-Term Storage : GNPDA2 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
1. Cat. No. 1305 - Human Kidney Tissue Lysate
Species Reactivity :H, M
GI Number : 19923881
Accession Number : NP_612208
Short Description : Glucosamine-6-phosphate deaminase 2
References

1. Wolosker H, Kline D, Bian Y, et al. Molecularly cloned mammalian glucosamine-6-phosphate deaminase localizes to transporting epithelium and lacks oscillin activity. FASEB J. 1998; 12:91-9.
2. Zhang J, Zhang W, Zou D, et al. Cloning and functional characterization of GNPI, a novel human homolog of glucosamine-6-phosphate isomerase/oscillin. J. Cell Biochem. 2003; 88:932-40.
3. Arreola R, Valderrama B, Morante ML, et al. Two mammalian glucosamine-6-phosphate deaminases: a structural and genetic study. FEBS Lett. 2003; 551:63-70.