Catalog# : 4697
The LASS (longevity assurance homolog) family members represent a subgroup of the homeobox gene family and are highly conserved from yeasts to mammals. Six members of this family of proteins have been characterized (LASS1-6) and all are involved in ceramide synthesis during cell growth regulation and cancer differentiation. LASS5, also called Trh4, is a 392 amino acid endoplasmic reticulum, multi-pass membrane protein. Functioning as a dihydro-ceramide synthase, LASS5 is involved in the production of sphingolipids containing mainly one fatty acid donor (N-linked palmitoyl-ceramide) in a fumonisin B1-independent manner. It uses palmitoyl-CoA as an acyl donor and is involved in the synthesis of C14, C16 and C18-ceramide. LASS5 is the most abundantly expressed and predominant ceramide synthase isoform in lung epithelia. Recent studies show that LASS5 partially correct growth and apoptosis. Multiple isoforms of LASS5 are known to exist. This antibody may cross-react with the highly homologous LASS6.
Additional Names : LASS5 (NT), Longevity Assurance Homolog 5, LAG1, TRH4
DescriptionLeft: Western blot analysis of LASS5 in rat brain tissue lysate with LASS5 antibody at (A) 1 and (B) 2 µg/ml.
Below: Immunohistochemistry of LASS5 in human brain tissue with LASS5 antibody at 2.5 μg/ml.
Other Product Images
Source : LASS5 antibody was raised against a 18 amino acid peptide near the amino terminus of the human LASS5.Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : LASS5 antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.
Application : LASS5 antibody can be used for detection of LASS5 by Western blot at 1 – 2 µg/ml.
Tested Application(s) : E, WB
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide : Cat.No. 4697P - LASS5 Peptide
Long-Term Storage : LASS5 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
1. Cat. No. 1463 - Rat Brain Tissue Lysate
Species Reactivity :H, M, R
GI Number : 22218345
Accession Number : NP_671723
Short Description : (NT) Longevity assurance homolog 5
References
1. Riebeling C, Allegood JC, Wang E, et al. Two mammalian longevity assurance gene (LAG1) family members, Trh1 and Trh, regulate dihydroceramide synthesis using different fatty acyl-CoA donors. J. Biol. Chem. 2003; 278:43452-9.
2. Lahiri S and Futerman AH. LASS5 is a bona fide dihydroceramide synthase that selectively utilizes palmitoyl-CoA as acyl donor. J. Biol Chem. 2005; 280:33735-8.
3. Spassieva S, Seo JG, Jiang JC, et al. 2006. Necessary role for the LAG1p motif in (dihydro)ceramide synthase activity. J. Biol. Chem. 2006; 281:33931-8.
4. Xu Z, Zhou J, McCoy DM, et al. LASS5 is the predominant ceramide synthase isoform involved in de novo sphingolipid synthesis in lung epithelia. J. Lipid Res. 2005; 46:1229-38.
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