Thursday, September 30, 2010

BAFF Receptor Antibody

BAFF Receptor Antibody

Catalog# : 3097

Members in the TNF superfamily regulate immune responses and induce apoptosis. A novel member in the TNF family was recently identified by several groups and designated BAFF, BLyS, TALL-1, THANK, and zTNF4 (1-4). BAFF/BLyS was characterized as a B cell activator since it induced B cell proliferation and immunoglobulin secretion (1,2). Two receptors, TACI and BCMA, for BAFF were originally identified (5). A third receptor was identified recently and designated BAFF-R and BR3 for BLyS receptor 3 (6,7). Unlike BCMA and TACI, which bind to BAFF and April, BAFF-R/BR3 is specific for BAFF and plays a predominant role in BAFF induced B cell development and survival. BAFF and its receptors are involved in B cell associated autoimmune diseases, and activate NF-kB and c-jun N-terminal kinase (4-6).
Additional Names : BAFF Receptor (CT), BAFF-R, BR3, BLyS
Description
Left: Western blot analysis of BAFF Receptor in human (H), mouse (M), and rat (R) spleen tissue lysates with BAFF Receptor antibody at 5 µg/ml.

Below: Immunohistochemistry of BAFF Receptor in human tonsil tissue with BAFF Receptor antibody at 5 µg/ml.

Other Product Images

Source : BAFF Receptor antibody was raised against a synthetic peptide corresponding to amino acids near the carboxy terminus of human BAFF Receptor The peptide sequence is identical between human and mouse origin.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : BAFF Receptor antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.
Immunogen : Human BAFF Receptor (C-Terminus) Peptide (Cat. No. 3097P)
Application : BAFF Receptor antibody can be used for detection of BAFF Rexeptor by Western blot at 2 to 5 µg/ml.
Tested Application(s) : E, WB, IHC
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide : Cat. No. 3097P - BAFF Receptor Peptide
Long-Term Storage : BAFF Receptor antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
1. Cat. No. 1306 - Human Spleen Tissue Lysate
2. Cat. No. 1406 - Mouse Spleen Tissue Lysate
3. Cat. No. 1466 - Rat Spleen Tissue Lysate
4. Cat. No. 1315 - Human Tonsil Tissue Lysate
Species Reactivity :H, M, R
GI Number : 15208475
Accession Number : AAK91826
Short Description : (CT) Receptor for BAFF
References
1. Moore PA, Belvedere O, Orr A, et al. BLyS: member of the tumor necrosis factor family and B lymphocyte stimulator. Science 1999;285:260-3
2. Schneider P, MacKay F, Steiner V, et al. BAFF, a novel ligand of the tumor necrosis factor family, stimulates B cell growth. J Exp Med 1999;189:1747-56
3. Shu HB, Hu WH, Johnson H. TALL-1 is a novel member of the TNF family that is down-regulated by mitogens. J Leukoc Biol 1999;65:680-3
4. Mukhopadhyay A, Ni J, Zhai Y, Yu GL, Aggarwal BB. Identification and characterization of a novel cytokine, THANK, a TNF homologue that activates apoptosis, nuclear factor-kappaB, and c-Jun NH2-terminal kinase. J Biol Chem 1999 ;274:15978-81

Nucleic Acid Conjugations


Bio-Synthesis offers chemical modifications of nucleic acids at specific sites within individual nucleotides or whithin oligonucleotides which allows various labels to be incorporated into DNA or RNA probes.

Modification process of Nucleic Acids
This modification process can further be use to conjugate with other molecules such as Peptide-oligonucleotide conjugation, Fluorescent oligonucleotide labeling, Non-fluorescent oligonucleotide labeling, Enzyme oligonucleotide conjugation, Oligonucleotide-HRP conjugations etc.

Use of Oligonucleotides
The use of oligonucleotides as therapeutic and diagnostic agents is growing rapidly with many compounds in preclinical and human clinical trials. In many of these applications the oligonucleotide is derivatized or conjugated with another molecular entity. These conjugations are typically performed for the purpose of attaching fluorescent dyes or other diagnostic reporter groups or for attaching compounds that modulate the activity or the pharmacokinetic behavior of the oligonucleotide.

Wednesday, September 29, 2010

BAFF Antibody

BAFF Antibody

Catalog# : 2221

Members in the TNF superfamily regulate immune responses and induce apoptosis. A novel member in the TNF family was recently identified by several groups and designated BAFF (for B cell Activating Factor belonging to the TNF Family), BLyS (for B Lymphocyte Stimulator), TALL-1 (for TNF- and ApoL-related Leukocyte-expressed Ligand), and THANK (for TNF Homologue that Activate Apoptosis, NF-kappaB and c-jun N-terminal Kinase) (1-4). BAFF/BLyS was characterized as a B cell activator since it induced B cell proliferation and immunoglobulin secretion (1,2). Three receptors for BAFF were recently identified and designated TACI, BCMA and BAFF-R (5,6). BAFF and its receptors are essential for B cell development, survival, and humoral immune responses. BAFF is involved in the development of autoimmune diseases including systemic lupus erythaematosus and rheumatoid arthritis (5).

Additional Names : BAFF (CT), BLyS, Thank, Tall
vDescription
Left: Western blot analysis of BAFF in human HL60 cell lysate (H) and mouse spleen tissue lysate (M) with BAFF antibody at 1 µg/ml.

Below: Immunocytochemistry of BAFF in HL60 cells with BAFF antibody at 1 µg/ml.

Other Product Images

Source : BAFF antibody was raised against a peptide corresponding to amino acids near the carboxy terminus of human BAFF.
Purification : Ion exchange chromatography purified
Clonality and Clone : This is a polyclonal antibody.
Host : BAFF antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.
Immunogen : Murine BAFF (C-Terminus) Peptide (Cat. No. 2221P)
Application : BAFF antibody can be used for detection of BAFF by Western blot at 0.25 to 1 µg/ml.
Tested Application(s) : E, WB, ICC
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide : Cat. No. 2221P - BAFF Peptide
Long-Term Storage : BAFF antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
1. Cat. No. 1205 - Jurkat Cell Lysate
2. Cat. No. 1406 - Mouse Spleen Tissue Lysate
Species Reactivity :H, M, R
GI Number : 5730097
Accession Number : NP_006564
Short Description : (CT) B cell Activating Factor in TNF Family
References
1. Moore PA , Belvedere O, Orr A, et al. BLyS: member of the tumor necrosis factor family and B lymphocyte stimulator. Science 1999;285:260-3
2. Schneider P, MacKay F, Steiner V, et al. BAFF, a novel ligand of the tumor necrosis factor family, stimulates B cell growth. J Exp Med 1999;189:1747-56
3. Shu HB, Hu WH, Johnson H. TALL-1 is a novel member of the TNF family that is down-regulated by mitogens. J Leukoc Biol 1999;65:680-3
4. Mukhopadhyay A, Ni J, Zhai Y, Yu GL, Aggarwal BB. Identification and characterization of a novel cytokine, THANK, a TNF homologue that activates apoptosis, nuclear factor-kappaB, and c-Jun NH2-terminal kinase. J Biol Chem 1999 ;274:15978-81

APRIL Antibody

APRIL Antibody

Catalog# : 2223

Members in the TNF superfamily regulate immune responses and induce apoptosis. A novel member in the TNF family was recently identified by several groups and designated APRIL (for a proliferation-inducing ligand), TALL-2 (for TNF- and ApoL-related Leukocyte-expressed Ligand 2), and TRDL-1alpha (for TNF related death ligand 1a) in human and mouse (1-4). Two receptors for APRIL were recently identified and designated TACI and BCMA (5-7). APRIL stimulates B and T cell proliferation, triggers humoral immune responses, activates NF-kappaB, and induces cell death (1-7). APRIL and its close relative of BlyS and their receptors BCMA and TACI are involved in diseases of autoimmunity and cancer (8,9). Additional Names : APRIL (ED), APRIL Description Left: Western blot analysis of APRIL expression in K562 cells with APRIL antibody at (A) 5 and (B) 10 µg/ml. Source : APRIL antibody was raised against a synthetic peptide corresponding to amino acids near the center of human APRIL. Purification : Affinity chromatography purified via peptide column Clonality and Clone : This is a polyclonal antibody. Host : APRIL antibody was raised in rabbit. Please use anti-rabbit secondary antibodies. Immunogen : Human APRIL (Extracellular Domain) Peptide (Cat. No. 2223P) Application : APRIL can be used for detection of APRIL by Western blot at 4-5 µg/ml.A 27 kDa band can be detected. Tested Application(s) : E, WB Buffer : Antibody is supplied in PBS containing 0.02% sodium azide. Blocking Peptide : Cat. No. 2223P - APRIL Peptide Long-Term Storage : APRIL antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. Positive Control 1. Cat. No. 1204 - K562 Cell Lysate Species Reactivity :H GI Number : 4507599 Accession Number : NP_003799 Short Description : (ED) A new member in the TNF family References 1. Hahne M, Kataoka T, Schroter M, et al. APRIL, a new ligand of the tumor necrosis factor family, stimulates tumor cell growth. J Exp Med. 1998;188(6):1185-90. 2. Shu HB, Hu WH, Johnson H. TALL-1 is a novel member of the TNF family that is down-regulated by mitogens. J Leukoc Biol. 1999;65(5):680-3. 3. Kelly K, Manos E, Jensen G, et al. APRIL/TRDL-1, a tumor necrosis factor-like ligand, stimulates cell death. Cancer Res. 2000;60(4):1021-7. 4. Yu G, Boone T, Delaney J, et al. APRIL and TALL-I and receptors BCMA and TACI: system for regulating humoral immunity. Nat Immunol. 2000;1(3):252-6.

APRIL Antibody

APRIL Antibody

Catalog# : 2223

Members in the TNF superfamily regulate immune responses and induce apoptosis. A novel member in the TNF family was recently identified by several groups and designated APRIL (for a proliferation-inducing ligand), TALL-2 (for TNF- and ApoL-related Leukocyte-expressed Ligand 2), and TRDL-1alpha (for TNF related death ligand 1a) in human and mouse (1-4). Two receptors for APRIL were recently identified and designated TACI and BCMA (5-7). APRIL stimulates B and T cell proliferation, triggers humoral immune responses, activates NF-kappaB, and induces cell death (1-7). APRIL and its close relative of BlyS and their receptors BCMA and TACI are involved in diseases of autoimmunity and cancer (8,9).
Additional Names : APRIL (ED), APRIL

Description
Left: Western blot analysis of APRIL expression in K562 cells with APRIL antibody at (A) 5 and (B) 10 µg/ml.






Source : APRIL antibody was raised against a synthetic peptide corresponding to amino acids near the center of human APRIL.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : APRIL antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.
Immunogen : Human APRIL (Extracellular Domain) Peptide (Cat. No. 2223P)
Application : APRIL can be used for detection of APRIL by Western blot at 4-5 µg/ml.A 27 kDa band can be detected.
Tested Application(s) : E, WB
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide : Cat. No. 2223P - APRIL Peptide
Long-Term Storage : APRIL antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
1. Cat. No. 1204 - K562 Cell Lysate
Species Reactivity :H
GI Number : 4507599
Accession Number : NP_003799
Short Description : (ED) A new member in the TNF family
References
1. Hahne M, Kataoka T, Schroter M, et al. APRIL, a new ligand of the tumor necrosis factor family, stimulates tumor cell growth. J Exp Med. 1998;188(6):1185-90.
2. Shu HB, Hu WH, Johnson H. TALL-1 is a novel member of the TNF family that is down-regulated by mitogens. J Leukoc Biol. 1999;65(5):680-3.
3. Kelly K, Manos E, Jensen G, et al. APRIL/TRDL-1, a tumor necrosis factor-like ligand, stimulates cell death. Cancer Res. 2000;60(4):1021-7.
4. Yu G, Boone T, Delaney J, et al. APRIL and TALL-I and receptors BCMA and TACI: system for regulating humoral immunity. Nat Immunol. 2000;1(3):252-6.

APRIL Antibody

APRIL Antibody

Catalog# : 2223

Members in the TNF superfamily regulate immune responses and induce apoptosis. A novel member in the TNF family was recently identified by several groups and designated APRIL (for a proliferation-inducing ligand), TALL-2 (for TNF- and ApoL-related Leukocyte-expressed Ligand 2), and TRDL-1alpha (for TNF related death ligand 1a) in human and mouse (1-4). Two receptors for APRIL were recently identified and designated TACI and BCMA (5-7). APRIL stimulates B and T cell proliferation, triggers humoral immune responses, activates NF-kappaB, and induces cell death (1-7). APRIL and its close relative of BlyS and their receptors BCMA and TACI are involved in diseases of autoimmunity and cancer (8,9).
Additional Names : APRIL (ED), APRIL

Description
Left: Western blot analysis of APRIL expression in K562 cells with APRIL antibody at (A) 5 and (B) 10 µg/ml.






Source : APRIL antibody was raised against a synthetic peptide corresponding to amino acids near the center of human APRIL.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : APRIL antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.
Immunogen : Human APRIL (Extracellular Domain) Peptide (Cat. No. 2223P)
Application : APRIL can be used for detection of APRIL by Western blot at 4-5 µg/ml.A 27 kDa band can be detected.
Tested Application(s) : E, WB
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide : Cat. No. 2223P - APRIL Peptide
Long-Term Storage : APRIL antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
1. Cat. No. 1204 - K562 Cell Lysate
Species Reactivity :H
GI Number : 4507599
Accession Number : NP_003799
Short Description : (ED) A new member in the TNF family
References
1. Hahne M, Kataoka T, Schroter M, et al. APRIL, a new ligand of the tumor necrosis factor family, stimulates tumor cell growth. J Exp Med. 1998;188(6):1185-90.
2. Shu HB, Hu WH, Johnson H. TALL-1 is a novel member of the TNF family that is down-regulated by mitogens. J Leukoc Biol. 1999;65(5):680-3.
3. Kelly K, Manos E, Jensen G, et al. APRIL/TRDL-1, a tumor necrosis factor-like ligand, stimulates cell death. Cancer Res. 2000;60(4):1021-7.
4. Yu G, Boone T, Delaney J, et al. APRIL and TALL-I and receptors BCMA and TACI: system for regulating humoral immunity. Nat Immunol. 2000;1(3):252-6.

APRIL Antibody

APRIL Antibody

Catalog# : 2415

Members in the TNF superfamily regulate immune responses and induce apoptosis. A novel member in the TNF family was recently identified by several groups and designated APRIL (for a proliferation-inducing ligand), TALL-2 (for TNF- and ApoL-related Leukocyte-expressed Ligand 2), and TRDL-1a (for TNF related death ligand 1a) in human and mouse (1-4). Two receptors for APRIL were recently identified and designated TACI and BCMA (5-7). APRIL stimulates B and T cell proliferation, triggers humoral immune responses, activates NF-kappaB, and induces cell death (1-7). APRIL and its close relative of BlyS and their receptors BCMA and TACI are involved in diseases of autoimmunity and cancer (8,9).
Additional Names : APRIL (ED2)

Description
Left: Western blot analysis of APRIL expression in Jurkat cells with APRIL antibody at 1 µg/ml (A), 2 µg/ml (B), and 4 µg/ml

Below: Immunohistochemistry of APRIL in human prostate tissue with APRIL antibody at 10 µg/ml.

Other Product Images
Source : APRIL antibody was raised against a synthetic peptide corresponding to amino acids within a extracellular domain of human APRIL.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : APRIL antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.
Immunogen : Human APRIL (Extracellular Domain 2) Peptide (Cat. No. 2415P)
Application : APRIL antibody can be used for detection of APRIL by Western blot at 1 – 4 µg/ml.Jurkat cell lysate was used as a positive control and a 27 kDa band was detected. APRIL antibody is human, mouse and rat reactive.
Tested Application(s) : E, WB, IHC
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide : Cat. No. 2415P - APRIL (ED2) Peptide
Long-Term Storage : APRIL antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
1. Cat. No. 1205 - Jurkat Cell Lysate
2. Cat. No. 1312 - Human Prostate Tissue Lysate
Species Reactivity :H, M
GI Number : 4507599
Accession Number : NP_003799
Short Description : (ED2) A new member in the TNF family
References
1. Hahne M, Kataoka T, Schroter M, et al. APRIL, a new ligand of the tumor necrosis factor family, stimulates tumor cell growth. J Exp Med. 1998;188(6):1185-90.
2. Shu HB, Hu WH, Johnson H. TALL-1 is a novel member of the TNF family that is down-regulated by mitogens. J Leukoc Biol. 1999;65(5):680-3.
3. Kelly K, Manos E, Jensen G, et al. APRIL/TRDL-1, a tumor necrosis factor-like ligand, stimulates cell death. Cancer Res. 2000;60(4):1021-7.
4. Yu G, Boone T, Delaney J, et al. APRIL and TALL-I and receptors BCMA and TACI: system for regulating humoral immunity. Nat Immunol. 2000;1(3):252-6.

Eotaxin Antibody

Eotaxin Antibody

Catalog# : 1123

Chemokines play a key role in inflammation. The CC chemokine eotaxin is a potent and specific eosinophil chemoattractant that is expressed by a variety of cell types in certain inflammatory conditions. Some G-protein coupled chemokine receptors are also utilized as virus coreceptors for fusion and infection of cells. The eotaxin receptor CCR3 is required for HIV-1 entry into target cells such as microglia and eotaxin inhibits the infection of HIV-1.
Additional Names : Eotaxin (CT), Eotaxin
Description
Left: Western blot analysis of eotaxin in HeLa cell lysate containing 10, 2.5, or 1 ng of full length recombinant eotaxin with eotaxin antibody at 1 µg/ml.

Below: Immunocytochemistry of Eotaxin in 293 cells with Eotaxin antibody at 5 µg/ml.

Other Product Images
Source : Eotaxin antibody was raised in rabbits against a peptide corresponding to amino acids near the carboxy terminus of human eotaxin.
Purification : Antibody is DEAE purified
Clonality and Clone : This is a polyclonal antibody.
Host : Eotaxin antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.
Immunogen : Human Eotaxin Peptide (Cat. No. 1123P)
Application : Eotaxin antibody can be used for detection of eotaxin by Western blot at 0.5 to 1 µg/ml.The recombinant protein can be used as positive control and monomer and homodimer are detected at approximate 9 and 18 kDa.
Tested Application(s) : E, WB, ICC
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide : Cat. No. 1123P - Eotaxin Peptide
Long-Term Storage : Eotaxin antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
1. Cat. No. 1201 - HeLa Cell Lysate
Species Reactivity :H
GI Number : 2462478
Accession Number : CAB07027
Short Description : (CT) CCR3 ligand
References
1. Kitaura M, Nakajima T, Imai T, et al. Molecular cloning of human eotaxin, an eosinophil-selective CC chemokine, and identification of a specific eosinophil eotaxin receptor, CC chemokine receptor 3. J Biol Chem 1996;271:7725-30
2. Ponath PD, Qin S, Ringler DJ, et al. Cloning of the human eosinophil chemoattractant, eotaxin. Expression, receptor binding, and functional properties suggest a mechanism for the selective recruitment of eosinophils. J Clin Invest 1996;97:604-12
3. He J, Chen Y, Farzan M, et al. CCR3 and CCR5 are co-receptors for HIV-1 infection of microglia. Nature. 1997;385(6617):645-9
4. Choe H, Farzan M, Sun Y, et al. The beta-chemokine receptors CCR3 and CCR5 facilitate infection by primary HIV-1 isolates. Cell. 1996;85:1135-48 (WD0500)

CXCR4 Antibody

CXCR4 Antibody

Catalog# : 1012

Human immunodeficiency virus (HIV) and related viruses require coreceptors, in addition to CD4, to infect target cells. Some G protein-coupled receptors including CCR5, CXCR4, CCR3, CCR2b and CCR8 in the chemokine receptor family, and four new human molecules GPR15, STRL33, GPR1 and V28 were recently identified as HIV coreceptors. Among them, CXCR4 (fusin, LESTR or HUMSTR) is a principal coreceptor for T-cell tropic strains of HIV-1 fusion and entry of human white blood cells. CXCR4 is also required for the infection by dual-tropic strains of HIV-1 and mediates CD-4 independent infection by HIV-2. The a-chemokine SDF-1 is the ligand for CXCR4 and prevents infection by T-tropic HIV-1. CXCR4 associates with the surface CD4-gp120 complex before HIV enters target cells. CXCR4 messenger RNA levels correlated with HIV-1 permissiveness in diverse human cell types. Antibodies to CXCR4 block HIV-1 and HIV-2 fusion and infection of human target cells. The amino-terminal domain and the second extracellular loop of CXCR4 serve as HIV biding sites.
Additional Names : CXCR4 (EL), CXCR4

Description
Left: Western blot analysis of CXCR4 in HeLa total cell lysate with anti-CXCR4 (EL) at 1:1000 dilution.




Source : CXCR4 antibody was raised against a peptide corresponding to amino acids 182 to 196 in the second extracellular loop (EL) of human CXCR4.
Purification : Antibody is DEAE purified
Clonality and Clone : This is a polyclonal antibody.
Host : CXCR4 antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.
Immunogen : Human CXCR4 (Extracellular Loop) Peptide (Cat. No. 1012P)
Application : CXCR4 antibody can be used for Western blot at 1:500 to 1:1000 dilution.
Tested Application(s) : E, WB
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide : Cat. No. 1012P - CXCR4 Peptide
Long-Term Storage : CXCR4 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
1. Cat. No. 1201 - HeLa Cell Lysate
Species Reactivity :H, M
GI Number : 4503175
Accession Number : NP_003458
Short Description : (EL) HIV & chemokine receptor
References
1. Dimitrov DS. Cell 1997;91:721-730
2. Feng Y et al. Science 1996;272:872-7
3. Berson JF et al. J Virol 1996;70:6288-95
4. Doranz BJ et al. Cell 1996;85:1149-1158

Custom Bioconjugations


Bio-Synthesis has acquired years of experience in chemical conjugation of peptides, proteins, oligonucleotides, lipid, biofunctional ligands, antibodies and other biological molecules onto solid surfaces. Our goal is to be your one expert source when seeking new solutions from early drug discovery to delivery.

Services
Bio-Synthesis provides services like: Nucleic acid and oligonucleotide modifications and conjugations, Peptide-oligonucleotide bioconjugations, Fluorescent Labels, Hapten-Carrier immunogen conjugation, Antibody modification and conjugation, Glycoconjuations, Immunotoxin conjugates, Liposome bioconjugations, Modification with synthetic polymers, Enzyme modification and conjugation.

Our capabilities
We are not only capable of synthesize synthetic molecules but also able to provide various bioconjugation systems for specific intended applications. Whether it is for tagging proteins to make them chromogenic or fluorescent, labeling molecules with biospecific ligands for subsequent affinity interactions, or cross linking two or more substances to create uniquely active conjugates, the choice are available in our laboratory.

Applications
At Bio-Synthesis, we assist client with determination of the appropriate methods for whatever your conjugation needs. As always - Quality is guaranteed! A myriad of application for bioconjugations have been used in area of: Developing assay of minute quantities of substance, The in vivo targeting of molecules, Modulation of specific biological process, Purification, detection or location of specific substance & Treatment of disease.

Tuesday, September 28, 2010

CXCR4 Antibody

CXCR4 Antibody

Catalog# : 1012
Human immunodeficiency virus (HIV) and related viruses require coreceptors, in addition to CD4, to infect target cells. Some G protein-coupled receptors including CCR5, CXCR4, CCR3, CCR2b and CCR8 in the chemokine receptor family, and four new human molecules GPR15, STRL33, GPR1 and V28 were recently identified as HIV coreceptors. Among them, CXCR4 (fusin, LESTR or HUMSTR) is a principal coreceptor for T-cell tropic strains of HIV-1 fusion and entry of human white blood cells. CXCR4 is also required for the infection by dual-tropic strains of HIV-1 and mediates CD-4 independent infection by HIV-2. The a-chemokine SDF-1 is the ligand for CXCR4 and prevents infection by T-tropic HIV-1. CXCR4 associates with the surface CD4-gp120 complex before HIV enters target cells. CXCR4 messenger RNA levels correlated with HIV-1 permissiveness in diverse human cell types. Antibodies to CXCR4 block HIV-1 and HIV-2 fusion and infection of human target cells. The amino-terminal domain and the second extracellular loop of CXCR4 serve as HIV biding sites.
Additional Names : CXCR4 (EL), CXCR4

Description
Left: Western blot analysis of CXCR4 in HeLa total cell lysate with anti-CXCR4 (EL) at 1:1000 dilution.




Source : CXCR4 antibody was raised against a peptide corresponding to amino acids 182 to 196 in the second extracellular loop (EL) of human CXCR4.
Purification : Antibody is DEAE purified
Clonality and Clone : This is a polyclonal antibody.
Host : CXCR4 antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.
Immunogen : Human CXCR4 (Extracellular Loop) Peptide (Cat. No. 1012P)
Application : CXCR4 antibody can be used for Western blot at 1:500 to 1:1000 dilution.
Tested Application(s) : E, WB
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide : Cat. No. 1012P - CXCR4 Peptide
Long-Term Storage : CXCR4 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
1. Cat. No. 1201 - HeLa Cell Lysate
Species Reactivity :H, M
GI Number : 4503175
Accession Number : NP_003458
Short Description : (EL) HIV & chemokine receptor
References
1. Dimitrov DS. Cell 1997;91:721-730
2. Feng Y et al. Science 1996;272:872-7
3. Berson JF et al. J Virol 1996;70:6288-95
4. Doranz BJ et al. Cell 1996;85:1149-1158

CXCR4 Antibody

CXCR4 Antibody

Catalog# : 1009

Human immunodeficiency virus (HIV) and related viruses require coreceptors, in addition to CD4, to infect target cells. Some G protein-coupled receptors including CCR5, CXCR4, CCR3, CCR2b and CCR8 in the chemokine receptor family, and four new human molecules GPR15, STRL33, GPR1 and V28 were recently identified as HIV coreceptors. Among them, CXCR4 (fusin, LESTR or HUMSTR) is a principal coreceptor for T-cell tropic strains of HIV-1 fusion and entry of human white blood cells. CXCR4 is also required for the infection by dual-tropic strains of HIV-1 and mediates CD-4 independent infection by HIV-2. The a-chemokine SDF-1 is the ligand for CXCR4 and prevents infection by T-tropic HIV-1. CXCR4 associates with the surface CD4-gp120 complex before HIV enters target cells. CXCR4 messenger RNA levels correlated with HIV-1 permissiveness in diverse human cell types. Antibodies to CXCR4 block HIV-1 and HIV-2 fusion and infection of human target cells. The amino-terminal domain and the second extracellular loop of CXCR4 serve as HIV binding sites.
Additional Names : CXCR4 (NT), CXCR4
Description
Left: Western blot analysis of CXCR4 in HeLa whole cell lysate with CXCR4 antibody at 0.5 µg/ml.

Below: Immunocytochemistry of CXCR4 in HeLa cells with CXCR4 antibody at 2 µg/ml.

Other Product Images


Source : CXCR4 antibody was raised against a peptide corresponding to amino acids near the amino terminus of human CXCR4.
Purification : Antibody is DEAE purified
Clonality and Clone : This is a polyclonal antibody.
Host : CXCR4 antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.
Immunogen : Human CXCR4 (N-Terminus) Peptide (Cat. No. 1009P)
Application : CXCR-4 antibody can be used for Western blot at 1 – 2 µg/ml dilution and for immunoprecipitation and immunocytochemistry at 10 µg/ml.
Tested Application(s) : E, WB, ICC, IP
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide : Cat. No. 1009P - CXCR4 Peptide
Long-Term Storage : CXCR4 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
1. Cat. No. 1201 - HeLa Cell Lysate
Species Reactivity :H, M
GI Number : 4503175
Accession Number : NP_003458
Short Description : (NT) HIV & chemokine receptor
References
1. Dimitrov DS. Cell 1997;91:721-730
2. Feng Y et al. Science 1996;272:872-7
3. Berson JF et al. J Virol 1996;70:6288-95
4. Doranz BJ et al. Cell 1996;85:1149-1158

CX3CR1 Antibody

CX3CR1 Antibody

Catalog# : 2201

CX3CR1 is one of the chemokine receptors that are required as coreceptors for HIV infection. The genes encoding human, mouse, and rat CX3CR1 were cloned and designated V28 and CMKBRL1, CX3CR1, and RBS11, respectively, (1-4). The encoded seven transmembrane protein was recently identified as the receptor for a novel transmembrane molecule, fractalkine, and renamed CX3CR1 (5). Recently, CX3CR1 was found to serve as a coreceptor for HIV-1 and HIV-2 envelope fusion and virus infection, which can be inhibited by fractokine (6). CX3CR1 mediates leukocyte migration and adhesion (5). CX3CR1 is expressed in a variety of human tissues and cell lines (2).

Additional Names : CX3CR1 (EL), V28
Description
Left: Western blot analysis of CX3CR1 in THP-1 cell lysate in the absence (lane A) or presence (lane B) of blocking peptide with CX3CR1 antibody at 1:500 dilution.

Below: Immunohistochemistry of CX3CR1 in human spleen cells with CX3CR1 antibody at 10 µg/ml.

Other Product Images
Source : CX3CR1 antibody was raised against a peptide corresponding to amino acids in a extracellular loop of human CX3CR1. The sequence is identical to that of rat CX3CR1 and differs from that of mouse CX3CR1 by one amino acid (3,4).
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : CX3CR1 antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.
Immunogen : Human CX3CR1 (Extracellular Loop) Peptide (Cat. No. 2201P)
Application : CX3CR1 antibody can be used for detection of CX3CR1 by Western blot at 1:500 dilution. THP-1 cell lysate can be used as positive control and an approximately 50 kDa band can be detected.
Tested Application(s) : E, WB, IHC
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide : Cat. No. 2201P - CX3CR1 Peptide
Long-Term Storage : CX3CR1 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
1. Cat. No. 1208 - THP-1 Cell Lysate
2. Cat. No. 1212 - 3T3 Whole Cell Lysate
Species Reactivity :H
GI Number : 1351394
Accession Number : P49238
Short Description : (EL) HIV & chemokine receptor
References
1. Raport CJ, Schweickart VL, Eddy RL JR, Shows TB, Gray PW. The orphan G-protein-coupled receptor-encoding gene V28 is closely related to genes for chemokine receptors and is expressed in lymphoid and neural tissues. Gene 1995;163:295-9
2. Combadiere C, Ahuja SK , Murphy PM. Cloning, chromosomal localization, and RNA expression of a human beta chemokine receptor-like gene. DNA Cell Biol 1995;14:673-80
3. Combadiere C, Gao J, Tiffany HL, Murphy PM. Gene cloning, RNA distribution, and functional expression of mCX3CR1, a mouse chemotactic receptor for the CX3C chemokine fractalkine. Biochem Biophys Res Commun 1998;253:728-32
4. Harrison JK, Barber CM, Lynch KR. cDNA cloning of a G-protein-coupled receptor expressed in rat spinal cord and brain related to chemokine receptors. Neurosci Lett 1994;169:85-9

CCR8 Antibody

CCR8 Antibody

Catalog# : 2097

CCR8 is one of the chemokine receptors that are required as coreceptors for HIV infection. The genes encoding human and murine CCR8 were cloned and designated TER1, CKR-L1, and ChemR1. The encoded seven transmembrane protein was identified as the receptor for human CC chemokine I-309 and renamed CCR8. Recently, CCR8 was found to serve as a coreceptor for diverse T-cell tropic, dual-tropic and macrophage-tropic HIV-1 strains. CCR8 mediates CC chemokine I-309 induced monocyte chemoattraction and HIV-1 envelope fusion and virus infection, which can be prevented by the CCR8 ligand I-309. CCR8 is expressed in spleen, thymus and T lymphoblastic cell lines.

Additional Names : CCR8 (EL), CCR8
Description
Left: Western blot analysis of CCR8 in human spleen lysate with CCR8 antibody at 1:500 dilution.


Source : CCR8 antibody was raised against a peptide corresponding to amino acids 183 to 201 of human CCR8, which locate in the second extracellular loop.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : CCR8 antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.
Immunogen : Human CCR8 (Extracellular Loop) Peptide (Cat. No. 2097P)
Application : CCR8 antibody can be used for detection of CCR8 by Western blot at 1:500 to 1:1000 dilution. An approximately 50 kDa band can be detected.
Tested Application(s) : E, WB
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide : Cat. No. 2097P - CCR8 Peptide
Long-Term Storage : CCR8 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
1. Cat. No. 1306 - Human Spleen Tissue Lysate
Species Reactivity :H
GI Number : 2231165
Accession Number : U45983
Short Description : (EL) HIV & chemokine receptor
References
1. Napolitano M, Zingoni A, Bernardini G, Spinetti G, Nista A, Storlazzi CT, Rocchi M, Santoni A. Molecular cloning of TER1, a chemokine receptor-like gene expressed by lymphoid tissues. J Immunol 1996;157:2759-63
2. Zaballos A, Varona R, Gutierrez J, Lind P, Marquez G. Molecular cloning and RNA expression of two new human chemokine receptor-like genes. Biochem Biophys Res Commun 1996;227:846-53
3. Samson M, Stordeur P, Labbe O, Soularue P, Vassart G, Parmentier M. Molecular cloning and chromosomal mapping of a novel human gene, ChemR1, expressed in T lymphocytes and polymorphonuclear cells and encoding a putative chemokine receptor. Eur J Immunol 1996;26:3021-8
4. Goya I, Gutierrez J, Varona R, Kremer L, Zaballos A, Marquez G. Identification of CCR8 as the specific receptor for the human beta-chemokine I-309: cloning and molecular characterization of murine CCR8 as the receptor for TCA-3. J Immunol 1998;160:1975-81

Lipid Oligonucleotide Conjugates

The availability of synthetic nucleic acids oligonucleotides and peptides, areas where BioSynthesis has extensive experience, has promoted the development of conjugates of these molecules cross-linked to compounds such as lipids, carbohydrates and small molecules (drugs) to yield products with distinct properties.

Lipid-oligonucleotide Conjugates
The main objective of cross-linking a lipid moiety to an oligonucleotide, either an oligodeoxynucleotide (ODN) or oligoribonucleotide (ORN), is to increase the hydrophobic character of the latter and its lipid-solubility. This way a conjugate would pass across the highly lipophilic cell membrane and into the cytosol, a process called transduction. Yet, depending on the lipid’s nature these conjugates may have also some other new biological properties.

Peptide-oligonucleotide Conjugates
Peptide-oligonucleotide conjugates have two moieties: a peptide that is covalently linked to an oligonucleotide: either an oligodeoxyribonucleotide (ODN) or an oligoribonucleotide (ORN). Alternatively, an oligonucleotide can be replaced with a bridged nucleic acid (BNA) that mimics the biological activity of that oligonucleotide. The oligonucleotide can interact with specific cell receptors to stimulate or inhibit biological responses, e.g. inhibition of a gene expression.

Carbohydrate-oligonucleotide Conjugates
These conjugates can have the carbohydrate moiety linked directly to the oligonucleotide or can be linked to a lipid or a peptide/protein. Due to the diversity of carbohydrate receptors present on the cell’s surface, oligosaccharides are useful for targeting conjugates to specific cells. Conjugation of antisense ODNs to a neoglycoprotein in which the protein has several mannose-6-phosphate residues, is readily internalized by cells having a receptor for this ligand on their surfaces.

Monday, September 27, 2010

CCR5 Antibody

CCR5 Antibody

Catalog# : 1112

Human immunodeficiency virus (HIV) and related virus require coreceptors, in addition to CD4, to infect target cells. Some G protein-coupled receptors including CCR5, CXCR4, CCR3, CCR2b and CCR8 in the chemokine receptor family, and four new human molecules GPR15, STRL33, GPR1 and V28 were recently identified as HIV coreceptors. Among them, CCR5 (CC-CKR-5) is a principal coreceptor for macrophage- and dual-tropic HIV-1 strains fusion and entry of human white blood cells. CCR5 is required for the infection by HIV-1, HIV-2, and SIV. The b-chemokines RANTES, MIP-1alpha and MIP-1beta are the ligands for CCR5 and prevent infection by M-tropic HIV-1. CXC5 associates with the surface CD4-gp120 of HIV complex and leads to membrane fusion and virus entry of target cells. The amino-terminal domain and the extracellular loops of CCR5 serve as HIV biding sites. CCR5 messenger RNA is expressed in lymphoid organs and monocytes.

Additional Names : CCR5 (NT), CCR5

Description
Left: Western blot analysis of CCR5 in THP-1 whole cell lysate with CCR5 antibody at 1:1000 dilution.

Below: Immunohistochemistry of CCR5 in human lymph node tissue with CCR5 antibody at 20 µg/ml.

Other Product Images

Source : CCR5 antibody was raised against a peptide corresponding to amino acids 6 to 20 of human CCR5 .
Purification : Antibody is DEAE purified
Clonality and Clone : This is a polyclonal antibody.
Host : CCR5 antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.
Immunogen : Human CCR5 (N-Terminus) Peptide (Cat. No. 1112P)
Application : CCR5 antibody can be used for Western blot at 1:1000 to 1:2000 dilution. An approximate 40 kDa band can be detected. CCR5 antibody can also detect CCR5 by immunohistochemistry at 20 µg/ml.
Tested Application(s) : E, WB, IHC
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide : Cat. No. 1112P - CCR5 Peptide
Long-Term Storage : CCR5 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
1. Cat. No. 1208 - THP-1 Cell Lysate
2. Cat. No. 1369 - Human Lymph Node Tissue Lysate
Species Reactivity :H
GI Number : 2104520
Accession Number : AAB57793
Short Description : (NT) HIV & chemokine receptor
References
1. Dimitrov, DS. Cell 1997;91:721-30
2. Littman, DR. Cell 1998;93:677-80
3. Deng, H, et al. Nature 1996;381:661-6
4. Dragic, T, et al. Nature 1996;381:667-73

CCR3 Antibody

CCR3 Antibody

Catalog# : 1109

Human immunodeficiency virus (HIV) and related virus require coreceptors to infect target cells. Some G protein-coupled receptors including CCR5, CXCR4, CCR3, CCR2b, CCR8, GPR15, STRL33, and CX3CR1 in the chemokine receptor family were recently identified as HIV coreceptors. CCR5, CXCR4 and CCR3 are the principal receptors for HIV fusion and entry of target cells (1-4). CCR3 facilitates infection by a subset of virus. CCR3 and CCR5 promote efficient infection of microglia, the major target cells in the CNS (3,4). High levels of CCR3 and CXCR4 expression were found on the neurons from both the central and peripheral nervous systems (5). The CCR3 ligand, eotaxin, and an anti-CCR3 antibody inhibited HIV infection of microglia. These results indicate CCR3 plays an important role in HIV infection of CNS.
Additional Names : CCR3 (NT), CC chemokine receptor 3, eosinophil eotaxin receptor

Description
Left: Western blot analysis of CCR3 in human spleen tissue lysates with CCR3 antibody at 1 (lane 1) and 2 µg/ml (lane 2), and 2 µg/ml in the presence of blocking peptide (lane 3).




Source : CCR3 antibody was raised against a 14 amino acid peptide from near the amino terminus of human CCR3.
Purification : Immunoaffinity purified IgG
Clonality and Clone : This is a polyclonal antibody.
Host : CCR3 antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.
Immunogen : Human CCR3 (N-Terminus) Peptide (Cat. No. 1109P)
Application : CCR3 antibody can be used for the detection of CCR3 by Western blot at 1 – 2 µg/ml.
Tested Application(s) : E, WB
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide : Cat. No. 1109P - CCR3 Peptide
Long-Term Storage : CCR3 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
1. Cat. No. 1306 - Human Spleen Tissue Lysate
Species Reactivity :H, M, R
GI Number : 30581170
Accession Number : NP_847899
Short Description : (NT) HIV and chemokine receptor
References
1. Feng Y, Broder CC, Kennedy PE, et al. HIV-1 entry cofactor: functional cDNA cloning of a seven-transmembrane, G protein-coupled receptor. Science 1996; 272:872-7.
2. Deng H, Liu R, Ellmeier W, et al. Identification of a major co-receptor for primary isolates of HIV-1. Nature 1996; 381:661-6.
3. Choe H, Farzan M, Sun Y, et al. The beta-chemokine receptors CCR3 and CCR5 facilitate infection by primary HIV-1 isolates. Cell 1996; 85:1135-48.
4. He J, Chen Y, Farzan M, et al. CCR3 and CCR5 are co-receptors for HIV-1 infection of microglia. Nature 1997; 385:645-9.

CCR3 Antibody

CCR3 Antibody

Catalog# : 1105

Human immunodeficiency virus (HIV) and related virus require coreceptors to infect target cells. Some G protein-coupled receptors including CCR5, CXCR4, CCR3, CCR2b, CCR8, GPR15, STRL33, and CX3CR1 in the chemokine receptor family were recently identified as HIV coreceptors. CCR5, CXCR4 and CCR3 are the principal receptors for HIV fusion and entry of target cells. CCR3 facilitates infection by a subset of virus. CCR3 and CCR5 promote efficient infection of microglia, the major target cells in the CNS (3,4). High levels of CCR3 and CXCR4 expression were found on the neurons from both the central and peripheral nervous systems. The CCR3 ligand, eotaxin, and an anti-CCR3 antibody inhibited HIV infection of microglia. These results indicate CCR3 plays an important role in HIV infection of CNS.

Additional Names : CCR3 (CT), CC chemokine receptor 3, eosinophil eotaxin receptor

Description
Left: Western blot analysis of CCR3 in human spleen tissue lysates with CCR3 antibody at 1 (lane 1) and 2 µg/ml (lane 2), and 2 µg/ml in the presence of blocking peptide (lane 3).

Below: Immunohistochemistry of CCR3 in human spleen tissue with CCR3 antibody at 10 µg/ml.

Other Product Images

Source : CCR3 antibody was raised against a 14 amino acid peptide from near the carboxy terminus of human CCR3.
Purification : Immunoaffinity purified IgG
Clonality and Clone : This is a polyclonal antibody.
Host : CCR3 antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.
Immunogen : Human CCR3 (C-Terminus) Peptide (Cat. No. 1105P)
Application : CCR3 antibody can be used for the detection of CCR3 by Western blot at 1 – 2 µg/ml.
Tested Application(s) : E, WB, IHC
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide : Cat. No. 1105P - CCR3 Peptide
Long-Term Storage : CCR3 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
1. Cat. No. 1306 - Human Spleen Tissue Lysate
Species Reactivity :H, M, R
GI Number : 30581170
Accession Number : NP_847899
Short Description : (CT) HIV and chemokine receptor
References
1. Feng Y, Broder CC, Kennedy PE, et al. HIV-1 entry cofactor: functional cDNA cloning of a seven-transmembrane, G protein-coupled receptor. Science 1996; 272:872-7.
2. Deng H, Liu R, Ellmeier W, et al. Identification of a major co-receptor for primary isolates of HIV-1. Nature 1996; 381:661-6.
3. Choe H, Farzan M, Sun Y, et al. The beta-chemokine receptors CCR3 and CCR5 facilitate infection by primary HIV-1 isolates. Cell 1996; 85:1135-48.
4. He J, Chen Y, Farzan M, et al. CCR3 and CCR5 are co-receptors for HIV-1 infection of microglia. Nature 1997; 385:645-9.

Bonzo Antibody

Bonzo Antibody

Catalog# : 2209

Human immunodeficiency virus (HIV) and simian immunodeficiency virus (SIV) require coreceptors, in addition to CD4, to infect target cells. Some G protein-coupled receptors including CCR5, CXCR4, CCR3, and CCR2b in the chemokine receptor family have been identified as HIV coreceptors. An orphan G protein-coupled receptor was recently cloned and designated Bonzo, STRL33 and TYMSTR, and identified as HIV and SIV coreceptor (1-4). Bonzo/STRL33 serves as coreceptor for SIV, HIV-2 and HIV-1. The messenger RNA of Bonzo/STRL33 is expressed in lymphoid tissues and activated peripheral blood lymphocytes.
Additional Names : Bonzo (NT2), STRL33

Description
Left: Western blot analysis of Bonzo in human spleen tissue lysate in the absence (lane A) or presence (lane B) of peptide with Bonzo antibody (NT2) at 1:500 dilution.

Below: Immunohistochemistry of Bonzo in human spleen cells with Bonzo antibody at 20 µg/ml.

Other Product Images
Source : Bonzo antibody was raised against a peptide corresponding to amino acids near the amino terminus of human Bonzo/STRL33. The sequence of this peptide differs from those of African green monkey and pig-tailed macaque by one or two amino acids, respectively,.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : Bonzo antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.
Immunogen : Human Bonzo (N-Terminus 2) Peptide (Cat. No. 2209P)
Application : Bonzo antibody can be used for detection of Bonzo by Western blot at 1:500 dilution.
Tested Application(s) : E, WB, IHC
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide : Cat. No. 2209P - Bonzo Peptide
Long-Term Storage : Bonzo antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
1. Cat. No. 1306 - Human Spleen Tissue Lysate
Species Reactivity :H
GI Number : 2209284
Accession Number : U73529
Short Description : (NT2) HIV coreceptor
References
1. Deng HK, Unutmaz D, KewalRamani VN, Littman DR. Expression cloning of new receptors used by simian and human immunodeficiency viruses. Nature 1997;388:296-300
2. Liao F, Alkhatib G, Peden KW, Sharma G, Berger EA, Farber JM. STRL33, A novel chemokine receptor-like protein, functions as a fusion cofactor for both macrophage-tropic and T cell line-tropic HIV-1. J Exp Med 1997;185:2015-23
3. Alkhatib G, Liao F, Berger EA, Farber JM, Peden KW. A new SIV co-receptor, STRL33. Nature 1997;388:238
4. Loetscher M, Amara A, Oberlin E, Brass N, Legler D, Loetscher P, D'Apuzzo M, Meese E, Rousset D, Virelizier JL, Baggiolini M, Arenzana-Seisdedos F, Moser B. TYMSTR, a putative chemokine receptor selectively expressed in activated T cells, exhibits HIV-1 coreceptor function. Curr Biol 1997;7:652-60 (RD0400)

ZBRK1 Antibody

ZBRK1 Antibody

Catalog# : 4817

ZBRK1 is a member of the Kruppel-associated box-zinc finger protein (KRAB-ZFP) protein family of transcriptional repressors. ZBRK1 has been shown to not only be up-regulated in senescent fibroblasts but also interacts with BRCA1, a transcriptional regulator with tumor suppressor activity. ZBRK1 binds to a specific sequence within the GADD45 gene, supporting the assembly of a nuclear complex containing BRCA1 and repressing its transcription. Recent experiments demonstrate that ZBRK1 can homo-oligomerize in vitro and in vivo through a C-terminal transcriptional repression domain (CTRD), and this oligomerization facilitates the ZBRK1-directed transcriptional repression through ZBRK1 response elements. These results suggest that higher order ZBRK1 oligomers may assemble through both protein-DNA and CTRD-dependent protein-protein interactions.
Additional Names : ZBRK1, Zinc finger protein 350, ZNF350
Description
Left: Western blot analysis of ZBRK1 in A-20 lysate with ZBRK1 antibody at 1 µg/ml in (A) the absence and (B) the presence of blocking peptide.




Source : ZBRK1 antibody was raised against a 20 amino acid peptide near the carboxy terminus of the human ZBRK1.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : ZBRK1 antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.
Application : ZBRK1 antibody can be used for detection of ZBRK1 by Western blot at 1 – 2 µg/ml.
Tested Application(s) : E, WB
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide : Cat.No. 4817P - ZBRK1 Peptide
Long-Term Storage : ZBRK1 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
1. Cat. No. 1288 - A20 Cell Lysate
Species Reactivity :H, M, R
GI Number : 59710107
Accession Number : NP_067645
Short Description : Zinc finger protein 350
References
1. Zheng L, Pan H, Li S, et al. Sequence-specific transcription corepressor function for BRCA1 through a novel Zinc finger protein, ZBRK1. Mol. Cell 2000; 6:757-68.
2. Collins T, Stone JR, and Williams AJ. All in the family: the BTB/POZ, KRAB, and SCAN domains. Mol. Cell. Biol. 2001; 21:3609-15.
3. Tan W, Kim S, and Boyer TG. Tetrameric oligomerization mediates transcriptional repression by the BRCA1-dependent Kruppel-associated Box-Zinc finger protein ZBRK1. J. Biol. Chem. 2004; 279:55153-60.

Quality Design Peptide Product

Peptide sequence selection is likely the most difficult step in the development of anti-peptide antibodies. Although the design of peptides for use as antigens is not exact, The following summary highlights some important points that should be considered in the design of a peptide. If you would like BSI to help you in designing your peptide, click here to request for sequence analysis or visit our Gizmo for online interative analysis. Remember! You design. We can Help!

1. Examine N– and C-terminus of the protein. If they are hydrophilic, they often constitute an excellent choice.

2. If both N– and C-terminus are suitable, choose internal hydrophilic region of the protein. Hydrophilic sequences have the best chances to reside at the surface of the protein and therefore to be accessible to the antibodies.

3. Avoid possible glycosylation or phosphorylation sites

4. Avoid a series of hydrophobic amino acids such as alanine, tryptophan etc.

5. Short synthetic peptides are quite flexible and are unable to mimic alpha helical sequences. However, run algorithms for predicting secondary structures and avoid regions which are strongly predicted alpha helical prior to peptide selection.

6. A peptide range between 10-20 amino acids is recommended. Shorter sequences have fewer chances to include good peptides, longer peptides tend to make stable secondary structures which are not necessarily the same in the protein of interest. Longer peptides also increase the chances of cross-reactions with other proteins.

7. A BLAST homology searches of the selected peptides against proteins of the organism obtained from in order to exclude unwanted cross-reactions to other proteins as much as possible. Less than 4-5 identical amino acids in a continuous stretch are required to exclude a cross-reaction.

8. Carrier protein coupling strategy needs to be taken into account. Alternatively, use the MAP strategy for synthesis of the peptide (not suitable for peptides from the C-terminus).

-Peptides from the C-terminus of the protein should be linked to the carrier through the N-terminus.
-Peptides from the N-terminus of the protein should be linked to the carrier by their C-terminus.
-Internal peptides should be coupled at the less hydrophilic end.
-N-terminal coupling is specific only if the peptide does not contain lysine residue.
-C-terminal coupling is specific only if the peptide does not contain aspartyl or glutamyl residues.
-If no specific coupling can be done, add cysteine at one end of the peptide. The only restriction is there should not be any other cysteines in the sequence.

9. Conjugation of a peptide to a carrier protein containing multiple epitopes, such as Keyhole-Limpet Hemocyanin (KLH) or Bovine Serum Albumin (BSA), are most common. KLH is preferred since it is more antigenic in the majority of animals. BSA is often used as a blocking reagent in assays, thus an anti-peptide antibody raised against a BSA conjugated peptide will show some specificity towards the buffer reagents. This can result in a false positive signal.

10. MAP is simply an alternative to KLH conjugation. It is recommended only for shorter peptides located either internally within a protein or at the N-terminus. KLH conjugation is recommended for a peptide at any location in a protein and when there are a cysteine residues within the peptide sequence.

11. Use at least 2 animals for each peptide since there is some genetic variability between animals.

12. Use more than 1 peptide for each protein in order to increase the chances for antibodies to recognize the protein.

The services for the separation of the antibodies produced against the individual peptides are available at Bio-Synthesis, call 800-227-0627 or write to us at support@biosyn.com for FREE consultation on peptide selection.

Sunday, September 26, 2010

List of Misidentified or contaminated cell lines

List of Misidentified or contaminated cell lines

Cell line cross contamination and misidentification have plagued biomedical research for as long as cells have been employed as resarch tools. A recent Science article indicated that in some cases, laboratories have invested substantial time and effort researching cell lines that were revealed to be misidentified.
(1). The situation has prompted the U.S. National Institutes of Health to issue a notice strongly urging researchers to authenticate cultured cells used in their laboratory.
(2). Many contaminated cell lines were overgrown by the contaminating cell line during establishment and so authentic stocks probably do not exist; in those cases all work has been performed on the contaminant, which may come from a different species, tissue or cell type.
Cell line cross-contamination was first discovered in the 1960s and has been extensively reported since that time. Contaminated or misidentified cell lines have also been listed on the websites of individual cell banks. To make it easier to check for these references, a single list has been developed summarising the cell lines known to be affected, their contaminants, and the references used.
The list was developed and is maintained by Dr. R. Ian Freshney and Dr. Armanda Capes-Davis. For additional background or citation, please refer to the associated article.
(3).This table is meant as a preliminary guide to avoiding suspect cell lines, but all recently acquired cell lines should be tested (e.g. by STR profiling for human cell lines) and compared to reference stock before use. Observations made in these lists are based on published reports and details obtained from cell banks, their websites, and Wikipedia. The authors take no credit nor responsibility for any of the primary observations and have merely attempted to collate data previously available on other sites.
Click on PDF link below for list of Misidentified or contaminated cell lines.
Reference:
1. Chatterjee, R. (2007) Cell biology. Cases of mistaken identity. Science 315, 928–31.
2. Ruiz Bravo, N. and Gottesman, M. (2007) Notice regarding authentication of cultured cell lines.
3. Capes-Davis, A., G. Theodosopoulos, I. Atkin, H. Drexler, A. Kohara, R.A.F. MacLeod, J.R. Masters, Y. Nakamura, Y.A. Reid, R.R. Reddel, R.I. Freshney. 2010. Check your cultures! A list of cross-contaminated or misidentified cell lines. International Journal of Cancer. 127:1–8.

List of Misidentified or contaminated cell lines

List of Misidentified or contaminated cell lines

Cell line cross contamination and misidentification have plagued biomedical research for as long as cells have been employed as resarch tools. A recent Science article indicated that in some cases, laboratories have invested substantial time and effort researching cell lines that were revealed to be misidentified.
(1). The situation has prompted the U.S. National Institutes of Health to issue a notice strongly urging researchers to authenticate cultured cells used in their laboratory.
(2). Many contaminated cell lines were overgrown by the contaminating cell line during establishment and so authentic stocks probably do not exist; in those cases all work has been performed on the contaminant, which may come from a different species, tissue or cell type.
Cell line cross-contamination was first discovered in the 1960s and has been extensively reported since that time. Contaminated or misidentified cell lines have also been listed on the websites of individual cell banks. To make it easier to check for these references, a single list has been developed summarising the cell lines known to be affected, their contaminants, and the references used.
The list was developed and is maintained by Dr. R. Ian Freshney and Dr. Armanda Capes-Davis. For additional background or citation, please refer to the associated article.
(3).This table is meant as a preliminary guide to avoiding suspect cell lines, but all recently acquired cell lines should be tested (e.g. by STR profiling for human cell lines) and compared to reference stock before use. Observations made in these lists are based on published reports and details obtained from cell banks, their websites, and Wikipedia. The authors take no credit nor responsibility for any of the primary observations and have merely attempted to collate data previously available on other sites.
Click on PDF link below for list of Misidentified or contaminated cell lines.
Reference:
1. Chatterjee, R. (2007) Cell biology. Cases of mistaken identity. Science 315, 928–31.
2. Ruiz Bravo, N. and Gottesman, M. (2007) Notice regarding authentication of cultured cell lines.
3. Capes-Davis, A., G. Theodosopoulos, I. Atkin, H. Drexler, A. Kohara, R.A.F. MacLeod, J.R. Masters, Y. Nakamura, Y.A. Reid, R.R. Reddel, R.I. Freshney. 2010. Check your cultures! A list of cross-contaminated or misidentified cell lines. International Journal of Cancer. 127:1–8.