Catalog# :4203
Presenilin1 was initially identified a marker of susceptibility to early-onset Alzheimer’s disease. In addition to PEN2, nicastrin and APH-1, Presenilin1 forms the g-secretase protein complex, a membrane-bound aspartyl protease that can cleave certain proteins at peptide bonds buried within the hydrophobic environment of the lipid bilayer. This cleavage is responsible for a key step in signaling from several cell-surface receptors and is thought to be required for the generation of the neurotoxic amyloid peptides that are central to the pathogenesis of Alzheimer’s disease. Like the tumor necrosis factor-a-converting enzyme (TACE) and the b-site cleavage enzyme (BACE) protease families, g-secretase will cleave the amyloid precursor protein (APP), but within the intramembrane region of APP, resulting in either the non-toxic p3 (from the a and g cleavage site) or the toxic Ab amyloid peptide (from the b and g cleavage site). It is thought that accumulation of the Ab peptide is the precursor to Alzheimer’s disease. Multiple isoforms of presenilin1 are known to exist. This antibody has no cross-reactivity to presenilin2.
Additional Names : Presenilin1, PSEN1, PS1, Gamma-secretase subunit presenilin-1
Description
Left:Western blot analysis of presenilin1 in human brain lysate with presenilin1 antibody at (A) 0.5, (B) 1, and (C) 2 µg/ml.
Below:Immunohistochemistry of Presenilin1 in human brain tissue with Presenilin1 antibody at 2.5 µg/ml.
Other Product Images
Source :Presenilin1 antibody was raised against a 23 amino acid peptide from near the carboxy terminus of human presenilin1.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : Presenilin1 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application : Presenilin1 antibody can be used for detection of presenilin1 by Western blot at 0.5 – 1 µg/ml.
Tested Application(s) : E, WB, IHC
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide :Cat.No. 4203P - Presenilin1 Peptide
Long-Term Storage : Presenilin1 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control :
1.Cat. No. 1303 - Human Brain Tissue Lysate
Species Reactivity : H, M, R
GI Number : 4506163
Accession Number : NP_000012
Short Description : a gamma-secretase subunit
References
1. Sherrington R, Rogaev EI, Liang Y, et al. Cloning of a gene bearing missense mutations in early-onset familial Alzheimer’s disease. Nature 1995; 375:754-60.
2. Weihofen A and Martoglio B. Intramembrane-cleaving proteases: controlled liberation of proteins and bioactive peptides. Trends Cell Biol. 2003; 13:71-8.
3. Periz G and Fortini ME. Functional reconstitution of g-secretase through coordinated expression of presenilin, nicastrin, aph-1, and pen-2. J. Neurosci. Res. 2004; 77:309-22.
4. Selkoe DJ. The cell biology of b-amyloid precursor protein and presenilin in Alzheimer’s disease. Trends Cell Biol. 1998; 8:447-53.
Description
Left:Western blot analysis of presenilin1 in human brain lysate with presenilin1 antibody at (A) 0.5, (B) 1, and (C) 2 µg/ml.
Below:Immunohistochemistry of Presenilin1 in human brain tissue with Presenilin1 antibody at 2.5 µg/ml.
Other Product Images
Source :Presenilin1 antibody was raised against a 23 amino acid peptide from near the carboxy terminus of human presenilin1.Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : Presenilin1 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application : Presenilin1 antibody can be used for detection of presenilin1 by Western blot at 0.5 – 1 µg/ml.
Tested Application(s) : E, WB, IHC
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide :Cat.No. 4203P - Presenilin1 Peptide
Long-Term Storage : Presenilin1 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control :
1.Cat. No. 1303 - Human Brain Tissue Lysate
Species Reactivity : H, M, R
GI Number : 4506163
Accession Number : NP_000012
Short Description : a gamma-secretase subunit
References
1. Sherrington R, Rogaev EI, Liang Y, et al. Cloning of a gene bearing missense mutations in early-onset familial Alzheimer’s disease. Nature 1995; 375:754-60.
2. Weihofen A and Martoglio B. Intramembrane-cleaving proteases: controlled liberation of proteins and bioactive peptides. Trends Cell Biol. 2003; 13:71-8.
3. Periz G and Fortini ME. Functional reconstitution of g-secretase through coordinated expression of presenilin, nicastrin, aph-1, and pen-2. J. Neurosci. Res. 2004; 77:309-22.
4. Selkoe DJ. The cell biology of b-amyloid precursor protein and presenilin in Alzheimer’s disease. Trends Cell Biol. 1998; 8:447-53.
1 comment:
Hi all,
Presenilins are a family of related multi-pass transmembrane proteins that function as a part of the gamma-secretase protease complex. The structure of presenilin-1 is still controversial, although recent research has produced a more widely accepted model. Thanks a lot!
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