Digoxigenin is a standard immunohistochemical marker for in situ hybridization. Bio-Synthesis provides digoxigenin labeled oligonucleotide by attaché this small hapten to an amino-modified oligos. Digoxigenin can be incorporated either, at both terminus, or within the sequence by using an amino-modifier either dT, C12 to create an attachment site for the digoxigenin moeity. The spacer arm between the nucleotide and the digoxigenin consists fo a 12-atom chain. Double HPLC purification is required for this labeling.
Molecular Probe
A molecular probe is a group of atoms or molecules attached to other molecules or cellular structures and used in studying the properties of these molecules and structures. Radioactive DNA or RNA sequences are used in molecular genetics to detect the presence of a complementary sequence by molecular hybridization.
Immunohistochemistry
Immunohistochemistry is a technique for identifying cellular or tissue constituents (antigens) by means of antigen-antibody interactions, the site of antibody binding being identified either by direct labeling of the antibody, or by use of a secondary labelling method.
Immunostaining
Immunostaining is a general term in biochemistry that applies to any use of an antibody-based method to detect a specific protein in a sample. The term immunostaining was originally used to refer to the immunohistochemical staining of tissue sections, as first described by Albert Coons in 1941.[ Now however, immunostaining encompasses a broad range of techniques used in histology, cell biology, and molecular biology that utilize antibody-based staining methods.
No comments:
Post a Comment