Catalog# :4275
Cellular senescence is the terminal non-dividing state that normal cells enter following completion of their proliferative potential. Fusions of immortal human cell lines with each other have led to their assignment to one of four complementation groups. Mortality factor 4 (MORF4) was identified as the lead member of a family of transcription factor-like proteins that reverses this immortal phenotype. Like other members in this family, MORF4 is localized to the nucleus and possesses transcription factor-like motifs such as helix-loop-helix and a leucine zipper motif that might allow it to form transcriptionally active homo- or heterodimers. MORF4 has been shown to bind to the transcription corepressors mSin3A and TLE, suggesting that together, these complexes may play a role in transcriptional repression of genes that lead to cellular senescence.
Additional Names : MORF4, Mortality factor 4, cellular senescence-related protein 1, SEN1
DescriptionLeft: Western blot analysis of MORF4 in K562 cell lysate with MORF4 antibody at (A) 1 and (B) 2 µg/ml.
Below: Immunohistochemistry of MORF4 in human brain tissue with MORF4 antibody at 5 µg/ml.
Other Product Images
Source :
MORF4 antibody was raised against a 18 amino acid peptide from near the amino terminus of human MORF4.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : MORF4 antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.
Application : MORF4 antibody can be used for the detection of MORF4 by Western blot at 1 µg/ml.
Tested Application(s) : E, WB, IHC
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide :Cat.No. 4275P - MORF4 Peptide
Long-Term Storage : MORF4 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control :
1. Cat. No. 1204 - K562 Cell Lysate
2. Cat. No. 1303 - Human Brain Tissue Lysate
Species Reactivity : H, M, R
GI Number : 14917016
Accession Number : Q9Y690
Short Description : a senescence-related protein
References
1. Pereira-Smith OM and Smith JR. Genetic analysis of indefinite division in human cells: identification of four complementation groups. Proc. Natl. Acad. Sci. USA 1988; 85:6042-6.
2. Bertram MJ, Berube NG, Hang-Swanson X, et al. Identification of a gene that reverses the immortal phenotype of a subset of cells and is a member of a novel family of transcription factor-like genes. Mol. Cell. Biol. 1999; 1479-85.
3. Yochum GS and Ayer DE. Role for the mortality factors MORF4, MRGX, and MRG15 in transcriptional repression via associations with Pfl1, mSin3A, and transducin-like enhancer of split. Mol. Cell. Biol. 2002; 22:7868-76.
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