Catalog# :4889
MettL7A belongs to the methyltransferase superfamily. It is a probable methyltransferase. Methyltransferase is a type of transferase enzyme which transfers a methyl group from a donor to an acceptor. Often methylation occurs on nucleic bases in DNA or amino acids in protein structures. DNA methylation is often utilized to silence and regulate genes without changing the original DNA sequence. DNA methylation may be necessary for normal growth from embryonic stages in mammals. When mutant embryonic stem cells lacking the murine DNA methyltransferase gene were introduced to a germline of mice they caused a recessive lethal phenotype. Methylation may also be linked to cancer development as methylation of tumor suppressor genes promotes tumorgenesis and metastasis. At least two isoforms of MettL7A are known to exisit. This antibody is predicted to not cross-react with MettL7B.
Additional Names : MettL7A (IN), Methyltransferase-like protein 7A
Description
Left: Western blot analysis of MettL7A in MCF cell lysate with MettL7A antibody at 2 µg/ml.
Below: Immunocytochemistry of MettL7a in MCF7 cells with MettL7a antibody at 2 µg/ml.
Other Product Images
Source :MettL7A antibody was raised against a 13 amino acid peptide near the center of human MettL7A.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : MettL7A antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.
Application : MettL7A antibody can be used for detection of MettL7A by Western blot at 2 – 4 µg/ml.
Tested Application(s) : E, WB, ICC
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide :Cat.No. 4889P - MettL7A Peptide
Long-Term Storage : MettL7A antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control :
1. Cat. No. 1219 - MCF7 Cell Lysate
Species Reactivity : H, M
GI Number : 89145417
Accession Number : NP_054752
Short Description : (IN) Methyltransferase-like protein 7A
References
1. Clark HF, Gurney AL, Abaya E, et al. The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment. Genome Res. 2003; 13:2265-70.
2. Li E, Bestor TH, and Jaenisch R. Targeted mutation of the DNA methyltransferase gene results in embryonic lethality. Cell 1992; 69:915-26.
3. Laird PW and Jaenisch R. DNA Methylation and Cancer. Human Molecular Genetics 1994; 3:1487-95.
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