Catalog# :4699
The nuclear pore complex (NPC) is a protein assembly localized at the nuclear rim and mediates macromolecular transport between the nucleus and the cytoplasm. The mammalian nucleoporin (NUP)-107 is part of the hetero-oligomeric complex that also contains NUP160, NUP133, NUP96, and mammalian homolog of yeast sec13p. While the majority of the NUP107-160 nuclear pore sub-complex localizes to the nuclear pore, a small fraction is observed at kinetochores and pro-metaphase spindle poles in mitotic cells in association with proteins such as Mad1, Mad2, Bub3 and Cdc20. Immunodepletion of the NUP107-160 complex resulted in defective spindle assembly indicating that it has multiple functions. NUP107 has recently been identified as an HIV dependency factor (HDF), suggesting that NUP107 may be an important drug target in HIV treatment. Multiple isoforms of NUP107 are known to exist.
Additional Names : NUP107 (IN), Nucleoporin 107, nuclear pore complex protein 107
Description
Left: Western blot analysis of NUP107 in A549 cell lysate with NUP107 antibody at (A) 1 and (B) 2 µg/ml.
Below: Immunocytochemistry of NUP107 in A549 cells with NUP107 antibody at 10 μg/ml.
Other Product Images
Source :NUP107 antibody was raised against a 20 amino acid peptide from near the center of human NUP107.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : NUP107 antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.
Application : NUP107 antibody can be used for detection of NUP107 by Western blot at 1 – 2 µg/ml.
Tested Application(s) : E, WB
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide :Cat.No. 4699P - NUP107 Peptide
Long-Term Storage : NUP107 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control :
1. Cat. No. 1203 - A549 Cell Lysate
Species Reactivity : H, M, R
GI Number : 12230339
Accession Number : P57740
Short Description : (IN) Nucleoporin 107
References
1. Tran EJ and Wente SR. Dynamic nuclear pore complex: life on the edge. Cell 2006; 125:1041-53.
2. Boehmer T, Enninga J, Dales S, et al. Depletion of a single nucleoporin, Nup107, prevents the assembly of a subset of nucleoporins into the nuclear pore complex. Proc. Natl. Acad. Sci. USA 2003; 100:981-5.
3. Orjalo AV, Arnaoutov A, Shen Z, et al. The Nup107-160 nucleoporin complex is required for correct bipolar spindle assembly. Mol. Bio. Cell 2006; 17:3806-18.
4. Brass AL, Dykxhoorn DM, Benita Y, et al. Identification of host proteins required for HIV infection through a functional genomic screen. Science 2008; 319:921-6.
No comments:
Post a Comment