Catalog# :4945
Glycosylphosphatidylinositol (GPI) lipid anchoring is an important post-translational modification of proteins that takes place in the endoplasmic reticulum. The synthesis of GPI is initiated by GPI-N-acetylglucosaminyltransferase (GPI-GnT), a complex of proteins including PIG-A, PIG-H, PIG-C, GPI1, and DPM2. PIG-Y, the mammalian homolog to yeast Eri1p, is also thought to be involved in the biosynthesis of GPI. The PIG-Y gene encodes two proteins, one of which arises from leaky scanning of the mRNA. This antibody only detects isoform 1 of PIG-Y. Despite its predicted molecular weight, PIG-Y often migrates at 28-30 kDa in SDS-PAGE.
Additional Names : PIG-Y (CT), Phosphatidylinositol glycan anchor biosynthesis class Y, PreY, PIGY
DescriptionLeft: Western blot analysis of PIG-Y in A-20 cell lysate with PIG-Y antibody at 2 µg/ml.
Below: Immunohistochemistry of PIGY in human spleen tissue with PIGY antibody at 2.5 µg/ml.
Other Product Images
Source : PIG-Y antibody was raised against a 16 amino acid peptide near the carboxy terminus of human PIG-Y.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : PIG-Y antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.
Application : PIG-Y antibody can be used for detection of PIG-Y by Western blot at 1 – 2 µg/ml.
Tested Application(s) : E, WB, IHC
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide :Cat.No. 4945P - PIG-Y Peptide
Long-Term Storage : PIG-Y antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control :
1. Cat. No. 1288 - A20 Cell Lysate
Species Reactivity : H, M
GI Number : 14249680
Accession Number : NP_116295
Short Description : (CT) Phosphatidylinositol glycan anchor biosynthesis class Y
References
1. Eisenhaber B, Maurer-Stroh S, Novatchkova M, et al. Enzymes and auxiliary factors for GPI lipid anchor biosynthesis and post-translational transfer to proteins. Bioessays 2003; 25:367-85.
2. Watanabe R, Murakami Y, Marmor MD, et al. Initial enzyme for glycosylphosphatidylinositol biosynthesis requires PIG-P and is regulated by DPM2. EMBO J. 2000; 19:4402-11.
3. Murakami Y, Siripanyaphinoyo U, Hong Y, et al. The initial enzyme for glycosylphosphatidylinositol biosynthesis requires PIG-Y, a seventh component. Mol. Biol. Cell 2005; 16:5236-46.
1 comment:
Hello,
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