Monday, August 22, 2011

KappaB ras Antibody


KappaB ras-1 (kappaB-ras-1) and kappaB-ras-2 are two small proteins that similar to Ras-like small GTPases that associate with IkappaB (IkappaB), an inhibitor of the transcription factor NF-kappaB. IkappaB exists in two homologous forms, IkappaB-alpha and IkappaB-beta, although IkappaB-beta contains a unique 47-amino acid region within its ankyrin domain. While inactive IkappaB-alpha-NF-kappaB complexes can shuttle in and out of the nucleus, IkappaB-beta-NF-kappaB complexes are retained exclusively in the cytoplasm. It is suggested that kappaB-ras proteins preferentially bind to the IkappaB-beta form through this unique insert within the ankyrin region, thus modulating the cellular location of IkappaB-beta and regulating the rate of degradation of IkappaB-beta. This antibody detects both kappaB-ras1 and kappaB-ras2.

Additional Names:
KappaB ras, NF-kappaB inhibitor-interacting Ras like protein, kappaB-ras, NKIRAS


Left: Western blot analysis of KappaB ras in 293 cell lysate with KappaB ras antibody at (A) 2 and (B) 4 µg/ml. Below: Immunohistochemistry of KappaB ras in human lymph node tissue with KappaB ras antibody at 1 µg/ml.

KappaB ras antibody was raised against a 18 amino acid peptide from near the center of human KappaB ras 1.
Purification: Affinity chromatography purified via peptide column
Clonality and Clone: This is a polyclonal antibody.
Host: KappaB ras antibody was raised in rabbit.
Application: KappaB ras antibody can be used for detection of KappaB ras by Western blot at 2 – 4 µg/ml.
Tested Application(s): E, WB, IHC
Buffer: Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide:Cat.No. 2491P - KappaB ras Peptide
Long-Term Storage: KappaB ras antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control:
1. Cat. No. 1210 - 293 Cell Lysate
2. Cat. No. 1369 - Human Lymph Node Tissue Lysate
Species Reactivity: H, M, R
GI Number: 7008402
Accession Number: AAF34998
Short Description: NF-kappaB inhibitor-interacting Ras-like protein
1. Fenwick C, Na SY, Voll RE, et al. A subclass of Ras proteins that regulate the degradation of IkappaB. Science 2000; 287:869-73.
2. Chen Y, Wu J and Ghosh G. KappaB-Ras binds to the unique insert within the ankyrin repeat domain if IkappaBbeta and regulates cytoplasmic retention of IkappaBbeta x NF-kappaB complexes. J. Biol. Chem. 2003; 278:23101-6.

No comments: