Tuesday, August 30, 2011

KappaB ras2 Antibody


Catalog#:2495

KappaB ras-1 (kappaB-ras-1) and kappaB-ras-2 are two small proteins that similar to Ras-like small GTPases that associate with IkappaB (IkappaB), an inhibitor of the transcription factor NF-kappaB. IkappaB exists in two homologous forms, IkappaB-alpha and IkappaB-beta, although IkappaB-beta contains a unique 47-amino acid region within its ankyrin domain. While inactive IkappaB-alpha-NF-kappaB complexes can shuttle in and out of the nucleus, IkappaB-beta-NF-kappaB complexes are retained exclusively in the cytoplasm. It is suggested that kappaB-ras proteins preferentially bind to the IkappaB-beta form through this unique insert within the ankyrin region, thus modulating the cellular location of IkappaB-beta and regulating the rate of degradation of IkappaB-beta. This antibody is specific for kappaB-ras2 and has no cross-reactivity to kappaB-ras1.

Additional Names: KappaB ras2, NF-kappaB inhibitor-interacting Ras like protein 2, kappaB-ras2, NKIRAS2

Description

Left: Western blot analysis of KappaB ras2 in RAW264.7 cell lysate with KappaB ras1 antibody at 1 µg/ml in the (A) absence and (B) presence of blocking peptide.





Source:KappaB ras2 antibody was raised against a 14 amino acid peptide from near the carboxy terminus of human KappaB ras2.
Purification: Affinity chromatography purified via peptide column
Clonality and Clone: This is a polyclonal antibody.
Host: KappaB ras2 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application: KappaB ras2 antibody can be used for detection of KappaB ras1 by Western blot at 0.5 – 1 µg/ml.
Tested Application(s): E, WB
Buffer: Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide:Cat.No. 2495P - KappaB ras2 Peptide
Long-Term Storage: KappaB ras2 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control:
1. Cat. No. 1283 - RAW264.7 Cell Lysate
Species Reactivity: H, M, R
GI Number: 19072794
Accession Number: NP_060065
Short Description: NF-kappaB inhibitor-interacting Ras-like protein
References
1. Fenwick C, Na SY, Voll RE, et al. A subclass of Ras proteins that regulate the degradation of IkappaB. Science 2000; 287:869-73.
2. Chen Y, Wu J and Ghosh G. KappaB-Ras binds to the unique insert within the ankyrin repeat domain if IkappaBbeta and regulates cytoplasmic retention of IkappaBbeta x NF-kappaB complexes. J. Biol. Chem. 2003; 278:23101-6.

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