Catalog# :4299
Protein-tyrosine kinases (PTKs) play important roles in regulating cell proliferation and differentiation during development. One member of the PTK family, PTK7, has been suggested to regulate the planar cell polarity (PCP) pathway in vertebrates and may play a role in neural convergent extension and neural tube closure. PTK7 has also been implicated in the development of cancer. Loss of PTK7 expression was seen in several melanoma cell lines and biopsies. Conversely, high-throughput analysis of acute myeloid leukemia samples showed an increased level of PTK7 expression compared to normal bone marrow and purified CD34+ cells. Multiple isoforms of PTK7 are known to exist.
Additional Names : PTK7 (NT), Protein tyrosine Kinase 7, colon carcinoma kinase-4, CCK-4
DescriptionLeft: Western blot analysis of PTK7 in (A) human, (B) mouse and (C) rat colon tissue lysate with PTK7 antibody at 1 µg/ml.
Below: Immunohistochemistry of PTK7 in human colon tissue with PTK7 antibody at 5 µg/ml.
Other Product Images
Source :PTK7 antibody was raised against a 15 amino acid peptide from near the amino terminus of human PTK7.Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : PTK7 antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.
Application : PTK7 antibody can be used for detection of PTK7 by Western blot at 0.5 – 1 µg/ml.
Tested Application(s) : E, WB, IHC
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide :Cat.No. 4299P - PTK7 Peptide
Long-Term Storage : PTK7 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control :
1. Cat. No. 1320 - Human Colon Tissue Lysate
Species Reactivity : H, M, R
GI Number : 47938093
Accession Number : AAH71557
Short Description : (NT) Protein tyrosine kinsase 7
References
1. Park SK, Lee HS and Lee ST. Characterization of the human full-length PTK7 cDNA encoding a receptor protein kinase-like molecule closely related to chick KLG. J. Biochem. 1996; 119:235-9.
2. Lu X, Borchers AG, Jolicoeur C, et al. PTK7/CCK-4 is a novel regulator of planar cell polarity in vertebrates. Nature 2004; 430:93-8.
3. Easty DJ, Mitchell PJ, Patel K, et al. Loss of expression of receptor tyrosine kinase family genes PTK7 and SEK in metastatic melanoma. Int. J. Cancer 1997; 1061-5.
4. Muller-Tidow C, Schwable J, Steffen B, et al. High-throughput analysis of genome-wide receptor tyrosine kinase expression in human cancers identifies potential novel drug targets. Clin. Cancer Res. 2004; 10:1241-9.
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