Sunday, January 30, 2011

SIRT2 Antibody

SIRT2 Antibody

Catalog# :4487

Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. ATG16, another member of the autophagy protein family, forms a complex with the ATG5-ATG12 conjugate. This multimeric protein has been shown to be essential for autophagosome formation in both yeast and mammals and targets the ATG5-ATG12 complex to the autophagic isolation membrane during the formation of the autophagosome. Because mammalian ATG16 has seven WD-repeats in its C-terminal domain, it has been suggested that these may form a platform for further protein-protein interactions. Multiple isoforms of ATG16 are known to exist.

Additional Names : SIRT2 (NT), NAD-dependent deacetylase sirtuin-2, SIR2L, SIR2L2, SIR2-like
Left: Western blot analysis of SIRT2 in human brain lysate with SIRT2 antibody at (A) 2.5 and (B) 5 µg/ml.

Below: Immunohistochemical staining of human brain tissue using Sirt2 antibody at 2.5 µg/ml.

Other Product Images

Source :SIRT2 antibody was raised against a 17 amino acid peptide near the amino terminus of the human SIRT2.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : SIRT2 antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.
Application : SIRT2 antibody can be used for detection of SIRT2 by Western blot at 2.5 – 5 µg/ml.
Tested Application(s) : E, WB, IHC
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide :Cat.No. 4487P - SIRT2 Peptide
Long-Term Storage : SIRT2 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control :
1. Cat. No. 1303 - Human Brain Tissue Lysate
Species Reactivity : H, M, R
GI Number : 13775600
Accession Number : NP_036369
Short Description : (NT) Sirtuin 2
1. Yamamoto H, Schoonjans K, and Auwerx J. Sirtuin functions in health and disease. Mol. Endocrinol. 2007; 21:1745-55.
2. Frye RA. Characterization of five human cDNAs with homology to the yeast SIR2 gene: SIR2-like proteins (sirtuins) metabolize NAD and may have protein ADP-ribosyltransferase activity. Biochem. Biophys. Res. Commun. 1999; 260:273-279.
3. North BJ, Marshall BL, Borra MT, et. al. The human Sir2 ortholog, SIRT2, is an NAD+-dependent tubulin deacetylase. Mol. Cell. 2003; 11:437-44.
4. Inoue T, Hiratsuka M, Osaki M, et al. The molecular biology of mammalian SIRT proteins: SIRT2 in cell cycle regulation. Cell Cycle 2007; 6:1011-8.

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