Catalog# : 2089
Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. DFF45/ICAD has been identified as inhibitor of caspase activated DNase DFF40/CAD. DFF45 related proteins CIDE-A and CIDE-B (for cell death-inducing DFF-like effector A and B) were recently identified (1). CIDE contains a new type of domain termed CIDE-N, which has high homology with the regulatory domains of DFF45/ICAD and DFF40/CAD (1,2). Expression of CIDE-A induces DNA fragmentation and activates apoptosis, which is inhibited by DFF45. CIDE-A is a DFF45-inhibitable effector that promotes cell death and DNA fragmentation. CIDE-A is expressed in many tissues.
Additional Names : CIDE-A (CT), CIDE-A
DescriptionLeft: Western blot analysis of CIDE-A in mouse heart tissue lysate with CIDE-A antibody at 1:500 dilution.
Below: Immunohistochemistry of CIDE-A in mouse heart tissue with CIDE-A antibody at 5 µg/ml.
Other Product Images
Source : CIDE-A antibody was raised against a peptide corresponding to amino acids 200 to 214 of mouse CIDE-A.Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : CIDE-A antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.
Immunogen : Mouse mCIDE-A (C-Terminus) Peptide (Cat. No. 2089P)
Application : CIDE-A antibody can be used for detection of CIDE-A by Western blot at 1:500 to 1:1000 dilution. An approximately 25 kDa band can be detected. It has no cross activity to CIDE-B.
Tested Application(s) : E, WB, IHC
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide : Cat. No. 2089P - CIDE-A Peptide
Long-Term Storage : CIDE-A antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
1.Cat. No. 1401 - Mouse Heart Tissue Lysate
Species Reactivity :M
GI Number : 3114592
Accession Number : AAC34985
Short Description : (CT) Cell death-Inducing DFF-like Effector
References
1.Inohara N, Koseki T, Chen S, Wu X, Nunez G. CIDE, a novel family of cell death activators with homology to the 45 kDa subunit of the DNA fragmentation factor. EMBO J 1998;17:2526-33
2.Inohara N, Koseki T, Chen S, Benedict MA, Nunez G. Identification of regulatory and catalytic domains in the apoptosis nuclease DFF40/CAD. J Biol Chem 1999 ;274:270-4 (RD1299)
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