DAPK2 Antibody

DAPK2 Antibody

Catalog# : 2323

Apoptosis is mediated by death domain containing adapter molecules and a caspase family of proteases. Certain serine/threonine protein kinases, such as RIP and DAP kinase, are mediators of apoptosis. DAP kinase (DAPK) is pro-apoptotic calcium-regulated serine/threonine kinase containing death domain. Ectopic expression of DAPK induces cell death and suppresses oncogenic transformation. DAPK mediates IFNg induced apoptosis. A novel DAP kinase-related protein was recently identified and designated DAPK2 and DRP-1 (1, 2). Ectopicly expressed DAPK2 induced apoptosis in various types of cells (1,2). DAPK has high sequence homology to ZIP kinase and DRAK1/2, and they represent a novel family of serine/threonine kinases, which mediates apoptosis through their catalytic activities. The messenger RNA of DAPK2 is expressed in multiple human tissues (1).

Additional Names : DAPK2

Description
Left: Western blot analysis of DAPK2 in A431 (H), mouse spleen (M), and rat kidney (R) lysates with DAPK2 antibody at 1 µg/ml.

Below: Immunohistochemistry of DAPK2 in mouse spleen cells with DAPK2 antibody at 2 µg/ml.

Other Product Images




Source : DAPK2 antibody was raised against a peptide corresponding to amino acids near the carboxy terminus of human DAPK2. The sequence of this antigenic peptide is identical to the corresponding amino acids of mouse origin (1,2).

Purification : Affinity chromatography purified via peptide column

Clonality and Clone : This is a polyclonal antibody.

Host : DAPK2 antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.

Immunogen : Human DAP kinase 2 Peptide (Cat. No. 2323P)

Application : DAPK2 antibody can be used for detection of DAPK2 by Western blot at 0.5 to 1 µg/ml.An approximately 42 kDa band can be detected. DAPK2 has no cross responses to DAPK1.

Tested Application(s) : E, WB, IHC

Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.

Blocking Peptide : Cat. No. 2323P - DAPK2 Peptide

Long-Term Storage : DAPK2 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Positive Control
1.Cat. No. 1202 - A431 Cell Lysate
2.Cat. No. 1406 - Mouse Spleen Tissue Lysate
3.Cat. No. 1465 - Rat Kidney Lysate

Species Reactivity :H, M, R

GI Number : 6521210

Accession Number : BAA88063

Short Description : Death-Associated Protein kinase 2

References
1.Kawai T, Nomura F, Hoshino K, Copeland NG, Gilbert DJ, Jenkins NA, Akira S. Death-associated protein kinase 2 is a new calcium/calmodulin-dependent protein kinase that signals apoptosis through its catalytic activity. Oncogene 1999;18(23):3471-80
2.Inbal B, Shani G, Cohen O, Kissil JL, Kimchi A. Death-associated protein kinase-related protein 1, a novel serine/threonine kinase involved in apoptosis. Mol Cell Biol 2000;20(3):1044-54 (WD0101)