CIDE-B Antibody

CIDE-B Antibody

Catalog# : 2319

Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. DFF45/ICAD has been identified as inhibitor of caspase activated DNase DFF40/CAD. DFF45 related proteins CIDE-A and CIDE-B (for cell death-inducing DFF-like effector A and B) were recently identified. CIDE contains a new type of domain termed CIDE-N, which has high homology with the regulatory domains of DFF45/ICAD and DFF40/CAD (1,2). Expression of CIDE-B induces apoptosis, which is inhibited by DFF45. CIDE-B is a DFF45-inhibitable effector that promotes cell death and DNA fragmentation. CIDE-B is expressed mainly in liver and at lower levels in spleen, kidney, peripheral blood lymphocytes and bone marrow. CIDE-B antibody has no cross activity to CIDE-A.

Additional Names : CIDE-B (C2), Cell death-inducing DFF-like effector B

Description
Left: Western blot analysis of CIDE-B in mouse small intestine tissue lysate with CIDE-B antibody at (A) 0.5, (B) 1 and (C) 2 µg/ml.

Below: Immunohistochemistry of CIDE-B in human small intestine tissue with CIDE-B antibody at 5 µg/ml.

Other Product Images
Source : CIDE-B antibody was raised against a peptide corresponding to 15 amino acids near the carboxy terminus of human CIDE-B.

Purification : Affinity chromatography purified via peptide column

Clonality and Clone : This is a polyclonal antibody.

Host : CIDE-B antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.

Application : CIDE-B antibody can be used for detection of CIDE-B by Western blot at 0.5 – 1 µg/ml.

Tested Application(s) : E, WB, IHC

Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.

Blocking Peptide : Cat.No. 2319P - CIDE-B Peptide

Long-Term Storage : CIDE-B antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Positive Control
1.Cat. No. 1408 - Mouse Small Intestine Tissue Lysate
2.Cat. No. 1308 - Human Small Intestine Tissue Lysate

Species Reactivity :H, M, R

GI Number : 6746627

Accession Number : AAF27658

Short Description : (C2) Cell death-inducing DFF-like effector B

References
1.Inohara N, Koseki T, Chen S,et al. CIDE, a novel family of cell death activators with homology to the 45 kDa subunit of the DNA fragmentation factor. EMBO J. 1998; 17:2526-33
2.Inohara N, Koseki T, Chen S, et al. Identification of regulatory and catalytic domains in the apoptosis nuclease DFF40/CAD. J. Biol. Chem. 1999; 274:270-4.