DAD1 Antibody

DAD1 Antibody

Catalog# : 3313

Defender of cell death 1 (DAD1) was initially discovered in BHK21 cells as a negative regulator of programmed cell death (1), a process important for normal organism development and tissue homeostasis (2). DAD1 was later shown to be a subunit of the mammalian oligosaccharyltransferase complex and is required for its function and structural integrity (3,4). Mice lacking DAD1 express abnormal N-linked glycoproteins and undergo increased apoptotic-associated embryonic death (5,6). Furthermore, overexpression of DAD1 mRNA is seen in some human hepatocellular carcinomas (7), indicating it may also play a role in carcinogenesis. It should be noted that DAD1 is not related to the inhibitor of apoptosis proteins (IAP) family and does not contain any baculoviral IAP repeat (BIR) domains.

Additional Names : DAD1

Description
Left: Western blot analysis of DAD1 in HepG2 cell lysate with DAD1 antibody at (A) 0.5, (B) 1, and (C) 2 µg/ml.

Below: Immunocytochemistry of DAD1 in HepG2 cells with DAD1 antibody at 10 µg/ml.

Other Product Images

Source : DAD1 antibody was raised against a peptide corresponding to 14 amino acids near the C-terminus of human DAD1.

Purification : Ion exchange chromatography purified

Clonality and Clone : This is a polyclonal antibody.

Host : DAD1 antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.

Immunogen : Murine DAD1 Peptide (Cat. No. 3313P)

Application : DAD1 antibody can be used for detection of DAD1 by Western blot at 0.5 to 2 µg/ml.Despite its predicted molecular weight, DAD1 migrates at ~22 kDa in SDS-PAGE.

Tested Application(s) : E, WB, ICC

Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.

Blocking Peptide : Cat. No. 3313P - DAD1 Peptide

Long-Term Storage : DAD1 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Positive Control
1.Cat. No. 1211 - HepG2 Cell Lysate

Species Reactivity :H, M

GI Number : 14602573

Accession Number : AAH09798

Short Description : An inhibitor of apoptosis

References
1.Nakashima T, Sekiguchi T, Kuraoka A, et al. Molecular cloning of a human cDNA encoding a novel protein, DAD1, whose defect causes apoptotic cell death in hamster BHK21 cells. Mol. Cell Biol. 1993; 13:6367-74.
2.Stellar H. Mechanisms and genes of cellular suicide. Science 1995; 267:1445-9.
3.Gilmore R and Kelleher DJ. DAD1, the defender against apoptotic cell death, is a subunit of the mammalian oligosaccharyltransferase. Proc. Natl. Acad. Sci. USA 1997; 94:4994-9.
4.Sanjay A, Fu J, and Kreibich G. DAD1 is required for the function and the structural integrity of the oligosaccharyltransferase complex. J. Biol. Chem. 1998; 273:26094-9.