DEDAF Antibody

DEDAF Antibody

Catalog# : 2227

Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD) death effector domain (DED), and caspase recruitment domain (CARD) containing molecules. Several molecules including caspases and adaptor FADD contain DEDs. A novel protein that interacts with DED of caspase-8 and 10, and FADD was identified recently and designated DEDAF for DED associated factor . DEDAF is identical to the transcriptional repressor RYBP (2). DEDAF/RYBP is expressed in multiple tissues and cell lines. DEDAF interacts with FADD and augments the formation of CD95/FADD/capase-8 complexes at the cell membrane, and interacts with DED-containing DNA biding protein (DEDD) in the nucleus indicating it is involved in the regulation of both cytoplasmic and nuclear events of apoptosis.

Additional Names : DEDAF
Description
Left: Western blot analysis of DEDAF expression in human A549 (lane A), HepG2 (lane B), and mouse 3T3 (lane C) cell lysates with DEDAF antibody at 1 µg /ml.

Below: Immunohistochemistry of DEDAF in mouse liver tissue with DEDAF antibody at 10 µg/ml.

Other Product Images

Source : DEDAF antibody was raised against a synthetic peptide corresponding to amino acids 215 to 228 of human DEDAF. The sequence is identical to that of mouse origin.

Purification : Affinity chromatography purified via peptide column

Clonality and Clone : This is a polyclonal antibody.

Host : DEDAF antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.

Immunogen : Human DEDAF Peptide (Cat. No. 2227P)

Application : DEDAF can be used for detection of DEDAF by Western blot at 0.5 to 1 µg/ml.A band at approximately 32 kDa can be detected.

Tested Application(s) : E, WB, IHC

Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.

Blocking Peptide : Cat. No. 2227P - DEDAF Peptide

Long-Term Storage : DEDAF antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Positive Control
1.Cat. No. 1203 - A549 Cell Lysate
2.Cat. No. 1211 - HepG2 Whole Cell Lysate
3.Cat. No. 1404 - Mouse Liver Tissue Lysate

Species Reactivity :H, M, R

GI Number : 5802963

Accession Number : AF179286

Short Description : A novel regulator of apoptosis

References
1.Zheng L, Schickling O, Peter ME, Lenardo MJ. The death effector domain-associated factor (DEDAF) plays distinct regulatory roles in the nucleus and cytoplasm. J Biol Chem. 2001;276(34):31945-52.
2.Garcia E, Marcos-Gutierrez C, del Mar Lorente M, Moreno JC, Vidal M. RYBP, a new repressor protein that interacts with components of the mammalian Polycomb complex, and with the transcription factor YY1. EMBO J. 1999;18(12):3404-18. (RD0201)