DcR1 Antibody

DcR1 Antibody

Catalog# : 2299

Apoptosis is induced by certain cytokines including TNF and Fas ligand in the TNF family through their death domain containing receptors. TRAIL/Apo2L is a new member of the TNF family and induces apoptosis of a variety of tumor cell lines. DR4 and DR5 are the recently identified functional receptors for TRAIL (1-3). Two decoy receptors for TRAIL have been identified and designated DcR1/TRID/TRAIL-R3/LIT (2-7) and DcR2/TRAIL-R4/TRUNDD (8-10). DcR1 has extracellular TRAIL-binding domain but lacks intracellular signaling domain. It is a glycophospholipid-anchored cell surface protein. DcR1 transcripts are expressed in many normal human tissues but not in most cancer cell lines. Overexpression of DcR1 did not induce apoptosis, but attenuated TRAIL-induced apoptosis.

Additional Names : DcR1, DcR1 (ED2), TRAIL-R3, TRID, LIT


Description
Left: Western blot analysis of DcR1 in HeLa cell (A), mouse (B) and rat (C) liver tissue lysates with DcR1 antibody at 1 µg/ml.

Below: Immunofluorescence of DcR1 in rat liver tissue with DcR1 antibody at 10 µg/ml.

Other Product Images


Source : DcR1 antibody was raised against a peptide corresponding to amino acids in the extracellular domain of human DcR1 precursor.

Purification : Affinity chromatography purified via peptide column

Clonality and Clone : This is a polyclonal antibody.

Host : DcR1 antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.

Immunogen : Human DcR1 Peptide (Cat. No. 2299P)

Application : DcR1 antibody can be used for detection of DcR1 by Western blot at 0.5 to 1 µg/ml.An approximate 65 kDa band can be detected.

Tested Application(s) : E, WB, IF

Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.

Blocking Peptide : Cat. No. 2299P - DcR1 Peptide

Long-Term Storage : DcR1 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Positive Control
1.Cat. No. 1201 - HeLa Cell Lysate
2.Cat. No. 1404 - Mouse Liver Tissue Lysate
3.Cat. No. 1464 - Rat Liver Tissue Lysate

Species Reactivity :H, M, R

GI Number : 2338422

Accession Number : AAB67104

Short Description : (ED2) Decoy Receptor 1 for TRAIL

References

1.Pan G; O'Rourke K; Chinnaiyan et al.. The receptor for the cytotoxic ligand TRAIL. Science; 1997;276:111-113
2.Pan G, Ni J, Wei YF, et al. An antagonist decoy receptor and a death domain-containing receptor for TRAIL. Science 1997;277:815-8
3.Sheridan JP, Marsters SA, Pitti RM, et al. A. Control of TRAIL-induced apoptosis by a family of signaling and decoy receptors. Science 1997;277:818-21
4.Degli-Esposti MA, Smolak PJ, Walczak H, et al, Smith CA. Cloning and characterization of TRAIL-R3, a novel member of the emerging TRAIL receptor family. J Exp Med 1997;186(7):1165-70