Daxx Antibody

Daxx Antibody

Catalog# : 1163

Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by certain cytokines including TNF and Fas ligand of the TNF family through their death domain containing receptors, TNFR1 and Fas. Cell death signals are transduced by death domain (DD)- containing adapter molecules and members of the ICE/CED-3 protease family. A novel DD-containing molecule was recently cloned from mouse, human and monkey and designated Daxx. Daxx binds specifically to the Fas death domain and enhances Fas induced apoptosis and activates the Jun N-terminal kinase (JNK) pathway. Daxx is widely expressed in fetal and adult human and mouse tissues indicating its important function in Fas signaling pathways.

Additional Names : Daxx (CT), Daxx

Description
Left: Western blot analysis of Daxx in HeLa total cell lysate with Daxx antibody at 1 mg/ml.

Below: Immunocytochemistry of DAXX in HeLa cells with DAXX antibody at 10 µg/ml.

Other Product Imagesc


Source : Daxx antibody was raised against a peptide corresponding to amino acids near the carboxy-terminus of human Daxx.

Purification : Antibody is DEAE purified

Clonality and Clone : This is a polyclonal antibody.

Host : Daxx antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.


Immunogen : Human Daxx (C-Terminus) Peptide (Cat. No. 1163P)
Application : Daxx antibody can be used for detection of Daxx by Western blot at 1 – 2 µg/ml.A 120 kDa major band can be detected.

Tested Application(s) : E, WB, ICC

Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.

Blocking Peptide : Cat. No. 1163P - Daxx Peptide

Long-Term Storage : Daxx antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Positive Control
1.Cat. No. 1201 - HeLa Cell Lysate

Species Reactivity :H, M

GI Number : 48146287

Accession Number : CAG33366

Short Description : (CT) Adapter Molecule

References
1.Yang X, Khosravi-Far R, Chang HY, Baltimore D. Daxx, a novel Fas-binding protein that activates JNK and apoptosis. Cell 1997;89:1067-1076
2.Kiriakidou M, Driscoll DA, Lopez-Guisa JM, Strauss JF 3rd. Cloning and expression of primate Daxx cDNAs and mapping of the human gene to chromosome 6p21.3 in the MHC region. DNA Cell Biol 1997;16:1289-1298 (RD1299)