IRF8 Antibody
Catalog# : 3401
Interferons (IFN)s are involved in a multitude of immune interactions during viral infections and play a major role in both the induction and regulation of innate and adaptive antiviral mechanisms (1 for review). During infection, host-virus interactions signal downstream molecules such as transcription factors such as IFN regulatory factor-3 (IRF3) which can act to stimulate transcription of IFN-alpha/beta genes. Unlike IRF3, IRF8 appears to act as a negative regulator of IFN-induced genes in most cases (2,3), but IRF8 mediates activation of NF-kappaB by the toll-like receptor 9 (TLR9) after stimulation by unmethylated CpG DNA in dendritic cells (4,5). Finally, it has been shown that IRF8 decreases bcl-2 expression and thus may play a role in chronic myelogenous leukemia (6).
Additional Names : IRF8
Description
Left: Western blot analysis of IRF8 in human thymus tissue lysate with IRF8 antibody at 1µg/ml in (A) the presence and (B) absence of blocking peptide.
Source : IRF8 antibody was raised against a peptide corresponding to 16 amino acids near the carboxy-terminus of human IRF8.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : IRF8 antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.
Immunogen : Human IRF8 Peptide (Cat. No. 3401P)
Application : IRF8 antibody can be used for detection of IRF8 by Western blot at 1 - 2 µg/ml.
Tested Application(s) : E, WB
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide : Cat. No. 3401P - IRF8 Peptide
Long-Term Storage : IRF8 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
1. Cat. No. 1314 - Human Thymus Tissue Lysate
Species Reactivity :H, M, R
GI Number : 4504567
Accession Number : NP_002154
Short Description : interferon regulatory factor 8
References
1. Malmgaard L. Induction and regulation of IFNs during viral infections. J. Interferon & Cyto. Res. 2004; 24:439-54
2. Weisz A, Marx P, Sharf R, et al. The human interferon consensus sequence binding protein (H-ICSBP) is a negative regulator of enhancer elements common to interferon inducible genes. J. Biol. Chem. 1992; 267:25589-96.
3. Nelson N, Marks MS, Driggers PH, et al. Interferon consensus sequence-binding protein, a member of the interferon regulatory factor family, suppresses interferon-induced gene transcription. Mol. Cell. Biol. 1993; 13:588-99.
4. Tsujimura H, Tamura T, Kong HJ, et al. Toll-like receptor 9 signaling activates NF-kappaB through IFN regulatory factor-8/IFN consensus sequence binding protein in dendritic cells. J. Immunol. 2004; 172:6820-7.
IRF3 Antibody
Catalog# : 3399
Interferons (IFN)s are involved in a multitude of immune interactions during viral infections and play a major role in both the induction and regulation of innate and adaptive antiviral mechanisms (1 for review). During infection, host-virus interactions signal downstream molecules such as transcription factors such as IFN regulatory factor-3 (IRF3) which can act to stimulate transcription of IFN-alpha/beta genes. IRF3 is present in an inactive form in the cytoplasm of most cells (2). Following viral infection, IRF3 can be activated by IkappaB kinase-epsilon and TANK-binding kinase 1 (TBK1) (3,4), whereupon IRF3 translocates to the nucleus. IRF3 can also be activated by stimulation of toll-like receptor 3 (TLR3) by dsRNA (3,5). IRF3 exists as at least two distinct isoforms.
Additional Names : IRF3
Description
Left: Western blot analysis of IRF3 in U937 whole cell lysate with IRF3 antibody at (A) 1, (B) 2, and (C) 4 µg/ml.
Below: Immunocytochemistry of IRF3 in Ramos cells with IRF3 antibody at 2 µg/ml.
Other Product Images
Source : IRF3 antibody antibody was raised against a peptide corresponding to 14 amino acids near the center of human IRF3.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : IRF3 antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.
Immunogen : Human IRF3 (Intermediate Domain) Peptide (Cat. No. 3399P)
Application : IRF3 antibody can be used for detection of IRF3 by Western blot at 1 - 2 µg/ml.
Tested Application(s) : E, WB, ICC
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide : Cat. No. 3399P - IRF3 Peptide
Long-Term Storage : IRF3 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
1. Cat. No. 1215 - U937 Cell Lysate
2. Cat. No. 1225 - Ramos Cell Lysate
Species Reactivity :H
GI Number : 4504725
Accession Number : NP_001562
Short Description : interferon regulatory factor 3
References
1. Malmgaard L. Induction and regulation of IFNs during viral infections. J. Interferon & Cyto. Res. 2004; 24:439-54.
2. Au WC, Moore PA, Lowther W, et al. Identification of a member of the interferon regulatory factor family that binds to the interferon-stimulated response element and activates expression of interferon-induced genes. Proc. Natl. Acad. Sci. USA 1995; 92:11657-61.
3. Fitzgerald KA, McWhirter SM, Faia KL, et al. IKKepsilon and TBK1 are essential components of the IRF3 signaling pathway. Nat. Immunol. 2003; 4:491-6.
4. Sharma S, Tenoever BR, Grandvaux N, et al. Triggering the interferon antiviral response through an IKK-related pathway. Science 2003; 300:1148-51.
BSI offer pre-miRNA precursor molecules which are single stranded, chemically modified and optimized nucleic acids, which designed to closely mimic endogenous mature microRNA (miRNA) molecules in cells. Our custom defined pre-miRNA precursor molecules provide flexible length and modifications for direct entry into the miRNA processing pathway and are treated identically to endogenous miRNA within cell.
Use of pre-miRNA
With the pre-miRNA, you can control specific miRNA activity, tightly regulate miRNA cellular levels, achieve optimal delivery efficiency with minimal cytotoxicity, miRNA enables up-regulation of miRNA activity for miRNA functional analysis, Since the Pre-miR miRNA Precursor Molecules directly enter the miRNA pathway, they eliminate any non-miRNA cellular response that is seen with siRNA-like designs. Unlike plasmid-based miRNA expression, the Pre-miR miRNA Precursor Molecules are easy to transfect with minimal cellular stress, and are able to be tightly regulated for specific dose response analysis.
All pre-miRNA oligonucleotide offer at BSI
All pre-miRNA oligonucleotide offer at BSI are: Deprotected, desalted and lyophilized, Every oligo is quality checked by MALDI-TOF mass spectrometry, Electrospray ionization mass spectrometry (ESI-MS), capillary electrophoresis (CE), and/or polyacrylamide gel electrophoresis (PAGE), Optional custom QC and preparative services available for in vivo application, Reliable quality and expertise in large-scale synthesis and QSR and cGMP manufacturing is available.
IRF3 Antibody
Catalog# : 3397
Interferons (IFN)s are involved in a multitude of immune interactions during viral infections and play a major role in both the induction and regulation of innate and adaptive antiviral mechanisms (1 for review). During infection, host-virus interactions signal downstream molecules such as transcription factors such as IFN regulatory factor-3 (IRF3) which can act to stimulate transcription of IFN-alpha/beta genes. IRF3 is present in an inactive form in the cytoplasm of most cells (2). Following viral infection, IRF3 can be activated by IkappaB kinase-epsilon and TANK-binding kinase 1 (TBK1) (3,4), whereupon IRF3 translocates to the nucleus. IRF3 can also be activated by stimulation of toll-like receptor 3 (TLR3) by dsRNA (3,5). IRF3 exists as at least two distinct isoforms.
Additional Names : IRF3 (CT), IRF3
Description
Left: Western blot analysis of IRF3 in Ramos whole cell lysate with IRF3 antibody at (A) 1, (B) 2, and (C) 4 µg/ml.
Below: Immunohistochemical staining of mouse kidney using IRF3 antibody at 2 µg/ml.
Other Product Images
Source : IRF3 antibody was raised against a peptide corresponding to 14 amino acids near the carboxy-terminus of human IRF3.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : IRF3 antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.
Immunogen : Human IRF3 (C-Terminus) Peptide (Cat. No. 3397P)
Application : IRF3 antibody can be used for detection of IRF3 by Western blot at 1 - 2 µg/ml.
Tested Application(s) : E, WB, IHC
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide : Cat. No. 3397P - IRF3 Peptide
Long-Term Storage : IRF3 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
1. Cat. No. 1225 - Ramos Cell Lysate
2. Cat. No. 1405 - Mouse Kidney Tissue Lysate
Species Reactivity :H, M
GI Number : 4504725
Accession Number : NP_001562
Short Description : (CT) interferon regulatory factor 3
References
1. Malmgaard L. Induction and regulation of IFNs during viral infections. J. Interferon & Cyto. Res. 2004; 24:439-54
2. Au WC, Moore PA, Lowther W, et al. Identification of a member of the interferon regulatory factor family that binds to the interferon-stimulated response element and activates expression of interferon-induced genes. Proc. Natl. Acad. Sci. USA 1995; 92:11657-61.
3. Fitzgerald KA, McWhirter SM, Faia KL, et al. IKKepsilon and TBK1 are essential components of the IRF3 signaling pathway. Nat. Immunol. 2003; 4:491-6.
4. Sharma S, Tenoever BR, Grandvaux N, et al. Triggering the interferon antiviral response through an IKK-related pathway. Science 2003; 300:1148-51.
IL-22 Receptor Antibody
Catalog# : 2497
A novel cytokine, designated IL-TIF for IL-10 related T cell-derived inducible factor and IL-22, was recently identified (1-3). The receptor for IL-22 (IL-22R, also termed CRF2-9 and IL-TIF-R1 chain) is a new member of the class II cytokine receptor family (3,4). IL-22R forms a complex with IL-10 receptor b chain and mediates IL-22 signaling. IL-22 and its receptor activate JAK-STAT signaling pathway. IL22R is expressed in normal liver and kidney and their cell lines HepG2 and TK-10. A soluble form of IL-22 receptor, also termed IL-22 binding protein (IL-22BP) and IL-22 receptor-a 2 (IL-22RA2), was identified very recently (5-7). IL-22BP prevents binding of IL-22 to the functional cell surface IL-22R complex and neutralizes IL-22 activity. LPS induces IL-22 expression, which indicates the role of IL-22 in inflammatory response.
Additional Names : IL-22 receptor (NT), IL22R
Description
Left: Western blot analysis of IL-22 receptor expression in human HepG2 cell lysate with IL-22 receptor antibody at 1 µg /ml.
Below: Immunocytochemistry of IL-22 receptor in HepG2 cells with IL-22 receptor antibody at 10 µg/ml.
Other Product Images
Source : IL-22 receptor antibody was raised against a synthetic peptide corresponding to amino acids near the amino terminus of human IL-22 receptor precursor .
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : IL-22 Receptor antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.
Immunogen : Human IL-22 Receptor (N-Terminus) Peptide (Cat. No. 2497P)
Application : IL-22 receptor antibody can be used for detection of IL-22 receptor by Western blot at 0.5 to 1 µg/ml.An approximately 62 kDa band can be detected.
Tested Application(s) : E, WB, ICC
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide : Cat. No. 2497P - IL-22 Receptor Peptide
Long-Term Storage : IL-22 Receptor antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
1. Cat. No. 1211 - HepG2 Cell Lysate
Species Reactivity :H, R
GI Number : 10719607
Accession Number : AF286095
Short Description : (NT) Interleukin 22 receptor
References
1. Dumoutier L, Louahed J, Renauld JC. Cloning and characterization of IL-10-related T cell-derived inducible factor (IL-TIF), a novel cytokine structurally related to IL-10 and inducible by IL-9. J Immunol. 2000;164(4):1814-9.
2. Dumoutier L, Van Roost E, Colau D, Renauld JC. Human interleukin-10-related T cell-derived inducible factor: molecular cloning and functional characterization as an hepatocyte-stimulating factor. Proc Natl Acad Sci USA . 2000 ;97(18):10144-9.
3. Xie MH, Aggarwal S, Ho WH, et al. Interleukin (IL)-22, a novel human cytokine that signals through the interferon receptor-related proteins CRF2-4 and IL-22R. J Biol Chem. 2000;275(40):31335-9.
4. Kotenko SV, Izotova LS, Mirochnitchenko OV, et al. Identification of the functional interleukin-22 (IL-22) receptor complex: J Biol Chem. 2001;276(4):2725-32.
IL-22 Receptor Antibody
Catalog# : 2489
A novel cytokine, designated IL-TIF for IL-10 related T cell-derived inducible factor and IL-22, was recently identified (1-3). The receptor for IL-22 (IL-22R, also termed CRF2-9 and IL-TIF-R1 chain) is a new member of the class II cytokine receptor family (3,4). IL-22R forms a complex with IL-10 receptor b chain and mediates IL-22 signaling. IL-22 and its receptor activate JAK-STAT signaling pathway. IL22R is expressed in normal liver and kidney and their cell lines HepG2 and TK-10. A soluble form of IL-22 receptor, also termed IL-22 binding protein (IL-22BP) and IL-22 receptor-a 2 (IL-22RA2), was identified very recently (5-7). IL-22BP prevents binding of IL-22 to the functional cell surface IL-22R complex and neutralizes IL-22 activity. LPS induces IL-22 expression, which indicates the role of IL-22 in inflammatory response.
Additional Names : IL-22 Receptor (CT), IL22R
Description
Left: Western blot analysis of IL-22 Receptor expression in human HepG2 cell lysate with IL-22 Receptor antibody at 1 µg /ml.
Description
Left: Western blot analysis of IL-22 Receptor expression in human HepG2 cell lysate with IL-22 Receptor antibody at 1 µg /ml. Source : IL-22 receptor antibody was raised against a synthetic peptide corresponding to amino acids 560 to 574 of human IL-22 receptor precursor.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : IL-22 Receptor antibody was raised in rabbit.Please use anti-rabbit secondary antibodies.
Immunogen : Human IL-22 Receptor (C-Terminus) Peptide (Cat. No. 2489P)
Application : IL-22 receptor antibody can be used for detection of IL-22 receptor by Western blot at 0.5 to 1 µg/ml.An approximately 62 kDa band can be detected.
Tested Application(s) : E, WB
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide : Cat. No. 2489P - IL-22 Receptor Peptide
Long-Term Storage : IL-22 Receptor antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
1. Cat. No. 1211 - HepG2 Cell Lysate
Species Reactivity :H, R
GI Number : 10719607
Accession Number : AF286095
Short Description : (CT) Interleukin 22 receptor
References
1. Dumoutier L, Louahed J, Renauld JC. Cloning and characterization of IL-10-related T cell-derived inducible factor (IL-TIF), a novel cytokine structurally related to IL-10 and inducible by IL-9. J Immunol. 2000;164(4):1814-9.
2. Dumoutier L, Van Roost E, Colau D, Renauld JC. Human interleukin-10-related T cell-derived inducible factor: molecular cloning and functional characterization as an hepatocyte-stimulating factor. Proc Natl Acad Sci USA . 2000 ;97(18):10144-9.
3. Xie MH, Aggarwal S, Ho WH, et al. Interleukin (IL)-22, a novel human cytokine that signals through the interferon receptor-related proteins CRF2-4 and IL-22R. J Biol Chem. 2000;275(40):31335-9.
4. Kotenko SV, Izotova LS, Mirochnitchenko OV, et al. Identification of the functional interleukin-22 (IL-22) receptor complex: J Biol Chem. 2001;276(4):2725-32.
IL-21 Receptor Antibody
Catalog# : 2471
A novel cytokine related to IL2 and IL15 was recently identified and designated IL-21 . The receptor for IL-21 (IL-21R, also termed NILR for novel Interleukin receptor) is a new member of the class I cytokine receptor family (1,2). IL-21R forms a complex with the common cytokine receptor g chain, gc, and mediates IL-21 signaling (3,4). Both IL-21R and the gc are necessary for the IL-21 function. IL-21 and its receptor activate JAK-STAT signaling pathway. IL-21R is expressed in spleen, thymus, natural killer (NK), T and B cell lines. IL-21 plays a role in the proliferation and maturation of NK, B and T cell populations.
Additional Names : IL-21 Receptor (NT), IL21R
Description
Left: Western blot analysis of IL-21 receptor expression in human Raji cell lysate with IL-21 receptor antibody at 1 µg /ml.
Source : IL-21 receptor antibody was raised against a synthetic peptide corresponding to amino acids 20 to 32 of human IL-21 receptor precursor.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : IL-21 Receptor antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.
Immunogen : Human IL-21 Receptor (N-Terminus) Peptide (Cat. No. 2471P)
Application : IL-21 receptor antibody can be used for detection of IL-21 receptor by Western blot at 0.5 to 1 µg/ml.An approximately 60 kDa band can be detected.
Tested Application(s) : E, WB
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide : Cat. No. 2471P - IL-21 Receptor Peptide
Long-Term Storage : IL-21 Receptor antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
1. Cat. No. 1207 - Raji Cell Lysate
Species Reactivity :H, M, R
GI Number : 11141869
Accession Number : NP_068570
Short Description : (NT) Interleukin 21 receptor
References
1. Parrish-Novak J, Dillon SR, Nelson A, et al. Interleukin 21 and its receptor are involved in NK cell expansion and regulation of lymphocyte function. Nature. 2000;408(6808):57-63.
2. Ozaki K, Kikly K, Michalovich D, et al. Cloning of a type I cytokine receptor most related to the IL-2 receptor beta chain. Proc Natl Acad Sci USA . 2000;97(21):11439-44.
3. Asao H, Okuyama C, Kumaki S, et al. the common gamma-chain is an indispensable subunit of the IL-21 receptor complex. J Immunol. 2001;167(1):1-5.
4. Vosshenrich CA, Di Santo JP. Cytokines: IL-21 joins the gamma(c)-dependent network Curr Biol. 2001;11(5):R175-7.
IL-21 Receptor Antibody
Catalog# : 2469
A novel cytokine related to IL-2 and IL-15 was recently identified and designated IL-21 . The receptor for IL-21 (IL-21R, also termed NILR for novel Interleukin receptor) is a new member of the class I cytokine receptor family (1,2). IL-21R forms a complex with the common cytokine receptor g chain, gc, and mediates IL-21 signaling (3,4). Both IL-21R and the gc are necessary for the IL-21 function. IL-21 and its receptor activate JAK-STAT signaling pathway. IL-21R is expressed in spleen, thymus, natural killer (NK), T and B cell lines. IL-21 plays a role in the proliferation and maturation of NK, B and T cell populations.
Additional Names : IL-21 Receptor (ED), IL21R
Description
Left: Western blot analysis of IL-21 receptor expression in human Raji cell lysate with IL-21 Receptor antibody at 1 µg /ml.
Below: Immunohistochemistry of IL-21 receptor in rat lung with IL-21 receptor antibody at 10 µg/ml.
Other Product Images
Source : IL-21 receptor antibody was raised against a synthetic peptide corresponding to amino acids 97 to 111 of human IL-21 receptor precursor .
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : IL-21 Receptor antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.
Immunogen : Human IL-21 Receptor (Extracellular Domain) Peptide (Cat. No. 2469P)
Application : IL-21 receptor antibody can be used for detection of IL-21 receptor by Western blot at 0.5 to 1 µg/ml.An approximately 60 kDa band can be detected.
Tested Application(s) : E, WB, IHC
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide : Cat. No. 2469P - IL-21 Receptor Peptide
Long-Term Storage : IL-21 Receptor antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
1. Cat. No. 1207 - Raji Cell Lysate
2. Cat. No. 1462 - Rat Lung Tissue Lysate
Species Reactivity :H, M, R
GI Number : 11141869
Accession Number : NP_068570
Short Description : (ED) Interleukin 21 receptor
References
1. Parrish-Novak J, Dillon SR, Nelson A, et al. Interleukin 21 and its receptor are involved in NK cell expansion and regulation of lymphocyte function. Nature. 2000;408(6808):57-63.
2. Ozaki K, Kikly K, Michalovich D, et al. Cloning of a type I cytokine receptor most related to the IL-2 receptor beta chain. Proc Natl Acad Sci USA . 2000;97(21):11439-44.
3. Asao H, Okuyama C, Kumaki S, et al. the common gamma-chain is an indispensable subunit of the IL-21 receptor complex. J Immunol. 2001;167(1):1-5.
4. Vosshenrich CA, Di Santo JP. Cytokines: IL-21 joins the gamma(c)-dependent network.Curr Biol. 2001;11(5):R175-7.
Antibody conjugates to another protein or molecule has be one of the most important applications in life science research, diagnostics and therapeutic studies. Bio-Synthesis has optimized viable methods for cross linking.
Cross-linking Antibody with Enzyme
There are various products formed after cross-linking of antibody with enzymes like: Horseradish peroxidase (HRP), Alkaline phosphatase (AP), ß-galactosidease (ß-gal), Glucose oxidase (GO)
Fluorescently Labeled Antibodies
There are various Fluorescently Labeled Antibodies formed after cross-linking of antibody with fluorescent such as: Cyanine dyes, Fluorescein, Rhodamine, Texas Red, Rminomethylcoumarin (AMCA), phycoerythin and many others.
IL-21 Antibody
Catalog# : 2465
A novel cytokine was recently identified in human and mouse and designated IL-21 , which has significant homology to IL-2, IL-4, and IL-15. The receptor for IL-21 (IL-21R, also termed NILR for novel Interleukin receptor) is a new member of the class I cytokine receptor family (1,2). IL-21R forms a complex with the common cytokine receptor g chain, gc, and mediates IL-21 signaling (3,4). IL-21 and its receptor activate JAK-STAT signaling pathway. IL-21 is expressed in activated T cells, and HL-60 and THP-1 cell lines. IL-21 plays a role in the proliferation and maturation of NK, B and T cell populations.
Additional Names : IL-21 (IN), IL-21
Description
Left: Western blot analysis of IL-21 expression in HL-60 cell lysate in the absence (lane A) or presence of blocking peptide (lane B) with IL-21 antibody at 1 µg /ml.
Below: Immunocytochemistry of IL-21 in K562 cells with IL-21 antibody at 2 µg/ml.
Other Product Images
Source : IL-21 antibody was raised against a synthetic peptide corresponding to 15 amino acids near the center of human IL-21 precursor .
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : IL-21 antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.
Immunogen : Human IL-21 (Intermediate Domain) Peptide (Cat. No. 2465P)
Application : IL-21 antibody can be used for detection of IL-21 by Western blot at 0.5 to 1 µg/ml.An approximately 18 kDa band can be detected.
Tested Application(s) : E, WB, ICC
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide : Cat. No. 2465P - IL-21 Peptide
Long-Term Storage : IL-21 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
1. Cat. No. 1209 - HL60 Cell Lysate
2. Cat. No. 1208 - THP-1 Whole Cell Lysate
Species Reactivity :H
GI Number : 11141875
Accession Number : NP_068575
Short Description : (IN) Interleukin 21, related to IL2 and IL15
References
1. Parrish-Novak J, Dillon SR, Nelson A, et al. Interleukin 21 and its receptor are involved in NK cell expansion and regulation of lymphocyte function. Nature. 2000;408(6808):57-63.
2. Ozaki K, Kikly K, Michalovich D, et al. Cloning of a type I cytokine receptor most related to the IL-2 receptor beta chain. Proc Natl Acad Sci USA . 2000;97(21):11439-44.
3. Asao H, Okuyama C, Kumaki S, et al. the common gamma-chain is an indispensable subunit of the IL-21 receptor complex. J Immunol. 2001;167(1):1-5.
