Tuesday, October 5, 2010

IRF3 Antibody

IRF3 Antibody

Catalog# : 3397

Interferons (IFN)s are involved in a multitude of immune interactions during viral infections and play a major role in both the induction and regulation of innate and adaptive antiviral mechanisms (1 for review). During infection, host-virus interactions signal downstream molecules such as transcription factors such as IFN regulatory factor-3 (IRF3) which can act to stimulate transcription of IFN-alpha/beta genes. IRF3 is present in an inactive form in the cytoplasm of most cells (2). Following viral infection, IRF3 can be activated by IkappaB kinase-epsilon and TANK-binding kinase 1 (TBK1) (3,4), whereupon IRF3 translocates to the nucleus. IRF3 can also be activated by stimulation of toll-like receptor 3 (TLR3) by dsRNA (3,5). IRF3 exists as at least two distinct isoforms.
Additional Names : IRF3 (CT), IRF3

Left: Western blot analysis of IRF3 in Ramos whole cell lysate with IRF3 antibody at (A) 1, (B) 2, and (C) 4 µg/ml.

Below: Immunohistochemical staining of mouse kidney using IRF3 antibody at 2 µg/ml.

Other Product Images
Source : IRF3 antibody was raised against a peptide corresponding to 14 amino acids near the carboxy-terminus of human IRF3.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : IRF3 antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.
Immunogen : Human IRF3 (C-Terminus) Peptide (Cat. No. 3397P)
Application : IRF3 antibody can be used for detection of IRF3 by Western blot at 1 - 2 µg/ml.
Tested Application(s) : E, WB, IHC
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide : Cat. No. 3397P - IRF3 Peptide
Long-Term Storage : IRF3 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
1. Cat. No. 1225 - Ramos Cell Lysate
2. Cat. No. 1405 - Mouse Kidney Tissue Lysate
Species Reactivity :H, M
GI Number : 4504725
Accession Number : NP_001562
Short Description : (CT) interferon regulatory factor 3
1. Malmgaard L. Induction and regulation of IFNs during viral infections. J. Interferon & Cyto. Res. 2004; 24:439-54
2. Au WC, Moore PA, Lowther W, et al. Identification of a member of the interferon regulatory factor family that binds to the interferon-stimulated response element and activates expression of interferon-induced genes. Proc. Natl. Acad. Sci. USA 1995; 92:11657-61.
3. Fitzgerald KA, McWhirter SM, Faia KL, et al. IKKepsilon and TBK1 are essential components of the IRF3 signaling pathway. Nat. Immunol. 2003; 4:491-6.
4. Sharma S, Tenoever BR, Grandvaux N, et al. Triggering the interferon antiviral response through an IKK-related pathway. Science 2003; 300:1148-51.

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