Monday, October 4, 2010

IL-21 Antibody

IL-21 Antibody

Catalog# : 2465

A novel cytokine was recently identified in human and mouse and designated IL-21 , which has significant homology to IL-2, IL-4, and IL-15. The receptor for IL-21 (IL-21R, also termed NILR for novel Interleukin receptor) is a new member of the class I cytokine receptor family (1,2). IL-21R forms a complex with the common cytokine receptor g chain, gc, and mediates IL-21 signaling (3,4). IL-21 and its receptor activate JAK-STAT signaling pathway. IL-21 is expressed in activated T cells, and HL-60 and THP-1 cell lines. IL-21 plays a role in the proliferation and maturation of NK, B and T cell populations.
Additional Names : IL-21 (IN), IL-21
Left: Western blot analysis of IL-21 expression in HL-60 cell lysate in the absence (lane A) or presence of blocking peptide (lane B) with IL-21 antibody at 1 µg /ml.

Below: Immunocytochemistry of IL-21 in K562 cells with IL-21 antibody at 2 µg/ml.

Other Product Images

Source : IL-21 antibody was raised against a synthetic peptide corresponding to 15 amino acids near the center of human IL-21 precursor .
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : IL-21 antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.
Immunogen : Human IL-21 (Intermediate Domain) Peptide (Cat. No. 2465P)
Application : IL-21 antibody can be used for detection of IL-21 by Western blot at 0.5 to 1 µg/ml.An approximately 18 kDa band can be detected.
Tested Application(s) : E, WB, ICC
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide : Cat. No. 2465P - IL-21 Peptide
Long-Term Storage : IL-21 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
1. Cat. No. 1209 - HL60 Cell Lysate
2. Cat. No. 1208 - THP-1 Whole Cell Lysate
Species Reactivity :H
GI Number : 11141875
Accession Number : NP_068575
Short Description : (IN) Interleukin 21, related to IL2 and IL15
1. Parrish-Novak J, Dillon SR, Nelson A, et al. Interleukin 21 and its receptor are involved in NK cell expansion and regulation of lymphocyte function. Nature. 2000;408(6808):57-63.
2. Ozaki K, Kikly K, Michalovich D, et al. Cloning of a type I cytokine receptor most related to the IL-2 receptor beta chain. Proc Natl Acad Sci USA . 2000;97(21):11439-44.
3. Asao H, Okuyama C, Kumaki S, et al. the common gamma-chain is an indispensable subunit of the IL-21 receptor complex. J Immunol. 2001;167(1):1-5.

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