Bcl-xL Antibody

Bcl-xL Antibody

Catalog# : 3341

Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Disruption of this process has been implicated in a variety of diseases such as cancer (reviewed in 1). Bcl-xL is a member of the Bcl-2 family of proteins that are critical regulators of apoptosis (reviewed in 2 and 3). These can be divided into two classes: those that inhibit apoptosis and those that promote cell death. Bcl-xL is an anti-apoptotic mitochondrial protein related to Bcl-w and the major transcript of the bcl-x gene (4). Its high expression in tumors is correlated with advanced disease and poor prognosis (5). Bcl-xL expression level increases in response to several stimuli such as ionizing radiation and treatment with chemotherapeutic agents (6,7).

Additional Names : Bcl-xL

Description
Left: Western blot analysis of Bcl-xL in A549 cell lysates with Bcl-xL antibody at (A) 1 and (B) 2 µg/ml.



Source : Bcl-xL antibody was raised against a peptide corresponding to 14 amino acids near the carboxy-terminus of human Bcl-xL.

Purification : Ion exchange chromatography purified

Clonality and Clone : This is a polyclonal antibody.

Host : Bcl-xL antibody was raised in rabbit.

Immunogen : Murine Bcl-xL Peptide (Cat. No. 3341P)

Tested Application(s) : E, WB

Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.

Blocking Peptide : Cat. No. 3341P - Bcl-xL Peptide

Long-Term Storage : Bcl-xL antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Positive Control
Cat. No. 1203 - A549 Cell Lysate

Species Reactivity :H

GI Number : 510901

Accession Number : CAA80661

Short Description : a member of the bcl-2 family

References

• Lockshin RA, Osborne B, and Zakeri Z. Cell death in the third millennium. Cell Death Differ. 2000; 7:2-7.
• Cory S, Huang DCS, and Adams JM. The Bcl-2 family: roles in cell survival and oncogenesis. Oncogene 2003; 22:8590-607.
• Heiser D, Labi V, Erlacher M, et al. The Bcl-2 protein family and its role in the development of neoplastic disease. Exp. Geron. 2004; 39:1125-35.
• Gonzalez-Garcia M, Perez-Ballestro R, Ding L et al. bcl-xL is the major bcl-x mRNA form expressed during murine development and its product localizes to mitochondria. Development 1994; 120:3033-42.