Beclin-1 Antibody

Beclin-1 Antibody

Catalog# : 3611

Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells (1,2). Beclin-1, a coiled-coil Bcl-2-interacting protein homologous to the yeast autophagy gene apg6 (3,4), is a mammalian autophagy gene that can inhibit tumorigenesis and is expressed at reduced levels in human breast carcinoma, suggesting that defects in autophagy proteins may contribute to the development or progression of tumors (5). Bcl-2 can bind to Beclin-1 and inhibit Beclin-1-dependent autophagy in yeast and mammalian cells, suggesting that Bcl-2 functions as an anti-autophagy protein as well as an anti-apoptotic protein, which helps maintain autophagy at levels that are more compatible with cell survival rather than cell death (6).

Additional Names : Beclin-1 (CT), Coiled-coil myosin-like Bcl-2-interacting protein
Description
Left: Western blot analysis of Beclin-1 in A431 cell lysate with Beclin-1 antibody at (A) 0.5, (B) 1 and (C) 2 µg/ml. Below: Immunocytochemistry staining of A431 cells using Beclin-1 antibody at 1 µg/ml.
Source :Beclin-1 antibody was raised against a 16 amino acid peptide from near the carboxy terminus of human Beclin-1.

Purification : Affinity chromatography purified via peptide column

Clonality and Clone : This is a polyclonal antibody.

Host : Beclin-1 antibody was raised in rabbit.

Immunogen : Human Beclin-1 / Coiled-coil myosin-like Bcl-2-interacting protein (C-Terminus) Peptide (Cat. No. 36

Tested Application(s) : E, WB, ICC

Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.

Blocking Peptide : Cat. No. 3611P - Beclin-1 Peptide

Long-Term Storage : Beclin-1 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Positive Control
Cat. No. 1202 - A-431 Cell Lysate

Species Reactivity :H, M

GI Number : 16307457

Accession Number : AAH10276

Short Description : (CT) An autophagy related protein

References

• Gozuacik D and Kimchi A. Autophagy as a cell death and tumor suppressor mechanism. Oncogene. 2004; 23:2891-906.
• Kisen GO, Tessitore L, Costelli P, et al. Reduced autophagic activity in primary rat hepatocellular carcinoma and ascites hepatoma cells. Carcinogenesis 1993; 14:2501-5.
• Liang XH, Kleeman LK, Jiang HH, et al. Protection against fatal Sindbis virus encephalitis by Beclin, a novel Bcl-2-interacting protein. J. Virol. 1998; 72:8586-96.
• Kametaka S, Okano T, Ohsumi M, et al. Apg14p and Apg6/Vps30p form a protein complex essential for autophagy in the yeast Saccharomyces cerevisiae. J. Biol. Chem. 1998; 273:22284-91.

For more detail regarding Peptide please visit here : Custom Peptide Synthesis