Catalog# : 3875
Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells and is caused by caspase activation. Proteins that comprise the Bcl-2 family appear to control the activation of these enzymes. One such member is multi-domain antiapoptotic protein Bfl-1, which is overexpressed in stomach and other cancers. Bfl-1 can interact with Bax and suppress apoptosis by inhibiting the release of cytochrome c and caspase-3 activation. It is upregulated in cisplatin-resistant human bladder tumors, suggesting that its expression may be important for cisplatin resistance and inhibition of apoptosis in cancer cells. At least two isoforms of Bfl-1 are known to exist. Presumably due to post-translational modifications, Bfl-1 is often observed at higher molecular weight in SDS-PAGE than its predicted molecular weight would suggest.
Additional Names : Bfl-1 (NT), Bcl-2-related protein A1, Hemopoietic-specific early response protein
DescriptionLeft: Western blot analysis of Bfl-1 in mouse kidney tissue lysate with Bfl-1 antibody at (A) 1 and (B) 2 µg/ml. Below: Immunohistochemistry of Bfl-1 in mouse kidney tissue with Bfl-1 antibody at 2 µg/ml.
Source : Bfl-1 antibody was raised against a 14 amino acid peptide from near the amino terminus of human Bfl-1.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : Bfl-1 antibody was raised in rabbit.
Tested Application(s) : E, WB
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide : Cat.No. 3875P - Bfl-1 Peptide
Long-Term Storage : Bfl-1 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
Cat. No. 1405 - Mouse Kidney Tissue Lysate
Species Reactivity :H, M, R
GI Number : 4757840
Accession Number : NP_004040
Short Description : (NT) Bcl-2, apoptosis, cell death, Bax
References
- Lockshin RA, Osborne B, and Zakeri Z. Cell death in the third millennium. Cell Death Differ. 2000; 7:2-7.
- Choi SS, Park IC, Yun JW, et al. A novel Bcl-2 related gene, Bfl-1, is overexpressed in stomach cancer and preferentially expressed in bone marrow. Oncogene 1995; 11:1693-8.
- Kim JK, Kim KD, Lee E, et al. Up-regulation of Bfl-1/A1 via NF-kappaB activation in cisplatin-resistant human bladder cancer cell line. Cancer Lett. 2004; 212:61-70.
- Zhang H, Cowan-Jacob SW, Simonen M, et al. Structural basis of BFL-1 for its interaction with BAX and its anti-apoptotic action in mammalian and yeast cells. J. Biol. Chem. 2000; 275:11092-9.
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