Bim Antibody

Bim Antibody

Catalog# : 2065

Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3 domain containing pro-apoptotic proteins, including Bad, Bax, Bid, Bik, and Hrk, form a growing subclass of the Bcl-2 family. A novel BH3 domain containing protein was recently identified and designated Bim or BOD in human, mouse and rat (1,2). Bim/BOD interacts with diverse members in the pro-survival Bcl-2 sub-family including Bcl-2, Bcl-xL and Bcl-w. Bim/BOD induces apoptosis. The messenger RNA of Bim is ubiquitously expressed in multiple tissues and cell lines (1,2).

Additional Names : Bim (IN), BOD
Description
Left: Western blot analysis of Bim in K562 (K) and A549 (A) whole cell lysates with Bim antibody at 1 µg/ml. Below: Immunohistochemistry of Bim in human skin cancer cells with Bim antibody at 20 µg/ml.

Source :Bim antibody was raised against a peptide corresponding to amino acids near the center of human Bim. The sequence is identical to that of mouse and differs from that of rat by one amino acid .

Purification : Affinity chromatography purified via peptide column

Clonality and Clone : This is a polyclonal antibody.

Host : Bim antibody was raised in rabbit.

Immunogen : Human Bim (Intermediate Domain) Peptide (Cat. No. 2065P)

Tested Application(s) : E, WB, IHC

Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.

Blocking Peptide : Cat. No. 2065P - Bim Peptide

Long-Term Storage : Bim antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Positive Control

• Cat. No. 1204 - K562 Cell Lysate
  

Species Reactivity :H, M, R

GI Number : 18202042

Accession Number : O43521

Short Description : (IN) A new member in Bcl-2 family

References

1. O'Connor L, Strasser A, O'Reilly LA, et al. Bim: a novel member of the Bcl-2 family that promotes apoptosis. EMBO J. 1998; 17:384-395.
2. Hsu SY, Lin P, and Hsueh AJ BOD (Bcl-2-related ovarian death gene) is an ovarian BH3 domain-containing proapoptotic Bcl-2 protein capable of dimerization with diverse antiapoptotic Bcl-2 members. Mol. Endocrinol. 1998; 12:1432-40.

For more detail regarding Peptides please visit here : Custom Peptide Synthesis