Bid Antibody

Bid Antibody

Catalog# : 3353

Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Disruption of this process has been implicated in a variety of diseases such as cancer (reviewed in 1). The Bcl-2 family of proteins is comprised of critical regulators of apoptosis that can be divided into two classes: those that inhibit apoptosis and those that promote cell death (reviewed in 2 and 3). Bid, a pro-apoptotic Bcl-2 family member, is cleaved by caspase-8 in response to apoptotic signals (4,5), exposing the Bcl-2 homology 3 (BH3) domain which is normally buried in the full-length protein (6). The cleaved complex is myris-toylated and translocated to the mitochondrial membrane where it may induce mitochondrial Bax and Bak to oligomerize (7,8).

Additional Names : Bid (CT), Bid

Description
Left: Western blot analysis of Bid in mouse lung cell lysates with Bid antibody at (A) 0.5, (B) 1, and (C) 2 µg/ml. Below: Immunohistochemical staining of mouse lung tissue using Bid antibody at 2 µg/ml.

Source : Bid antibody was raised against a peptide corresponding to 14 amino acids near the carboxy-terminus of human Bid.

Purification : Ion exchange chromatography purified

Clonality and Clone : This is a polyclonal antibody.

Host : Bid antibody was raised in rabbit.

Immunogen : Human Bid (C-Terminus) Peptide (Cat. No. 3353P)

Tested Application(s) : E, WB, IHC

Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.

Blocking Peptide : Cat. No. 3353P - Bid Peptide

Long-Term Storage : Bid antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Positive Control
Cat. No. 1402 - Mouse Lung Tissue Lysate

Species Reactivity :H, M

GI Number : 54673639

Accession Number : AAH36364

Short Description : (CT) a member of the bcl-2 family

References

1. Lockshin RA, Osborne B, and Zakeri Z. Cell death in the third millennium. Cell Death Differ. 2000; 7:2-7.
2. Cory S, Huang DCS, and Adams JM. The Bcl-2 family: roles in cell survival and oncogenesis. Oncogene 2003; 22:8590-607.
3. Heiser D, Labi V, Erlacher M, et al. The Bcl-2 protein family and its role in the development of neoplastic disease. Exp. Geron. 2004; 39:1125-35.
4. Wang K, Yin XM, Chao DT, et al. BID: a novel BH3 domain-only death agonist. Genes Dev. 1996; 10:2859-69.

For more detail regarding Peptide please visit here : Custom Peptide Synthesis