Bmf Antibody

Bmf Antibody

Catalog# : 3031

Apoptosis is related to many diseases and development. Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3-only proteins, including Bad, Bid, Bik, Hrk, Bim, Noxa, and PUMA, form a growing subclass of the Bcl-2 family. A novel BH3-only protein was recently identified in human and mouse and designated Bmf (for Bcl-2-modifing factor) (1). The BH3 domain in Bmf is required both for binding to Bcl-2 proteins and for triggering apoptosis. In healthy cells, Bmf associates with the dynein light chain 2 (DLC2) component of the myosin V motors and is sequestered by the cell's actin cytoskeleton. Disruption of the actin cytoskeleton, either by depolymerization of actin filaments or by detachment of cells from the extracellular matrix, triggers release and activation of Bmf, initiating the downstream apoptotic program (1,2). Bmf is constitutively expressed in many tissues (1,2).

Additional Names : Bmf (NT), Bmf
Description
Left: Western blot analysis of Bmf expression in human HepG2 (lane 1) and 293 (lane 2) cell lysates with Bmf antibody at 2 µg /ml. Below: Immunocytochemistry of Bmf in HeLa cells with Bmf antibody at 10 µg/ml.

Source :EndoG antibody was raised with a synthetic peptide corresponding to 15 amino acids near the amino terminus of human EndoG.

Purification : Affinity chromatography purified via peptide column

Clonality and Clone : This is a polyclonal antibody.

Host : Bmf antibody was raised in rabbit.

Immunogen : Human Bmf (N-Terminus) Peptide (Cat. No. 3031P)

Tested Application(s) : E, WB, ICC

Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.

Blocking Peptide : Cat. No. 3031P - Bmf Peptide

Long-Term Storage : Bmf antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Positive Control

• Cat. No. 1211 - HepG2 Cell Lysate
• Cat. No. 1210 - 293 Whole Cell Lysate
• Cat. No. 1201 - HeLa Cell Lysate
  

Species Reactivity :H, M

GI Number : 15723378

Accession Number : NP_277038

Short Description : (NT) A new member in Bcl-2 family

References

1. Puthalakath H, Villunger A, O'Reilly LA, Beaumont JG, Coultas L, Cheney RE, Huang DC, Strasser A. Bmf: a proapoptotic BH3-only protein regulated by interaction with the myosin V actin motor complex, activated by anoikis. Science. 2001;293(5536):1829-32.
2. Hunt A, Evan G. Apoptosis. Till death us do part. Science. 2001;293(5536):1784-5.