Bnip3L Antibody

Bnip3L Antibody

Catalog# : 2289

Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3 domain containing pro-apoptotic proteins, including Bad, Bax, Bid, Bik, Hrk, Nip3, and Bim, form a growing subclass of the Bcl-2 family. A novel BH3 domain containing protein was recently identified and designated Bnip3L, Bnip3alpha, and Nix (for Nip3-like protein X) (1-3). Bnip3L/Bnip3alpha/Nix is a homolog of the E1B 19K/Bcl-2 binding and pro-apoptotic protein Bnip3. Overexpression of Bnip3L induces apoptosis (2,3). Bnip3L interacts with and overcomes suppresses by Bcl-2 and Bcl-xL. Bnip3L is localized in mitochondria. The messenger RNA of Bnip3L is ubiquitously expressed in human tissues (1,2). Bnip3L and Bnip3 form a new subfamily of the pro-apoptotic mitochondrial proteins.

Additional Names : Bnip3L, Nix
Description
Left: Western blot analysis of Bnip3L in K562 whole cell lysate in the absence (A), or presence (B) of immunogenic peptide (2289P) with Bnip3L antibody (IN) at 1 µg/ml. Below: Immunohistochemical staining of human kidney tissue using Bnip3L antibody at 2 µg/ml.

Source :Bnip3L antibody was raised against a peptide corresponding to amino acids 77 to 92 of human origin, which are identical to those of mouse Bnip3L.

Purification : Affinity chromatography purified via peptide column

Clonality and Clone : This is a polyclonal antibody.

Host : Bnip3L antibody was raised in rabbit.

Immunogen : Human Bnip3L (Intermediate Domain) Peptide (Cat. No. 2289P)

Tested Application(s) : E, WB, IHC

Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.

Blocking Peptide : Cat. No. 2289P - Bnip3L Peptide

Long-Term Storage : Bnip3L antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Positive Control

• Cat. No. 1204 - K562 Cell Lysate
• Cat. No. 1305 - Human Kidney Tissue Lysate
  

Species Reactivity :H

GI Number : 4138825

Accession Number : AF067396

Short Description : A new member in Bcl2 family

References

1. Matsushima M, Fujiwara T, Takahashi E, et al. Isolation, mapping, and functional analysis of a novel human cDNA (BNIP3L) encoding a protein homologous to human NIP3. Genes Chromosomes Cancer 1998;21:230-5
2. Yasuda M, Han JW, Dionne CA, Boyd JM, Chinnadurai G. BNIP3alpha: a human homolog of mitochondrial proapoptotic protein BNIP3. Cancer Res 1999;59:533-7
3. Chen G, Cizeau J, Vande Velde C, et al. Nix and Nip3 form a subfamily of pro-apoptotic mitochondrial proteins. J Biol Chem 1999;274(1):7-10
4. Imazu T, Shimizu S, Tagami S, et al. Bcl-2/E1B 19 kDa-interacting protein 3-like protein (Bnip3L) interacts with bcl-2/Bcl-xL and induces apoptosis by altering mitochondrial membrane permeability. Oncogene. 1999;18:4523-9 (RD1200)

For more detail regarding Peptide please visit here : Custom Peptide Synthesis