Bif Antibody

Bif Antibody

Catalog# : 3817

Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells and is caused by the activation of proteolytic enzymes termed caspases. Proteins that comprise the Bcl-2 family such as Bax appear to control the activation of these enzymes. Bax activity was found to be regulated by its association with Bax-interacting factor 1 (BIF), a member of the endophilin B family that is associated with intracellular membranes.. Following this interaction, Bax undergoes a conformational change and translocates to mitochondrial membranes. The Bax/BIF interaction appears to be enhanced by apoptotic stimuli, suggesting that BIF acts as the trigger to activate Bax, and as suppression of BIF promoted HeLa cell colony formation in soft agar, it may have a role in the suppression of cancer progression. At least two isoforms of BIF are known to exist.

Additional Names : Bif, Bax-interacting factor 1, endophilin B1
Description
Left: Western blot analysis of BIF in HeLa cell lysate with BIF antibody at (A) 1 and (B) 2 µg/ml. Below: Immunocytochemistry of BIF in HeLa with BIF antibody at 10 µg/ml.



Source :BIF antibody was raised against a 15 amino acid peptide from near the carboxy terminus of human BIF.

Purification : Affinity chromatography purified via peptide column

Clonality and Clone : This is a polyclonal antibody.

Host : Bif antibody was raised in rabbit.

Tested Application(s) : E, WB, ICC

Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.

Blocking Peptide : Cat.No. 3817P - Bif Peptide

Long-Term Storage : Bif antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Positive Control
Cat. No. 1201 - HeLa Cell Lysate

Species Reactivity :H, M

GI Number : 13469879

Accession Number : AAK27365

Short Description : a member of the Bcl-2 family

References

1. Lockshin RA, Osborne B, and Zakeri Z. Cell death in the third millennium. Cell Death Differ. 2000; 7:2-7.
2. Oltvai ZN, Milliman CL, and Korsmeyer SJ. Bcl-2 heterodimerizes in vivo with a conserved homolog, Bax, that accelerates programmed cell death. Cell 1993; 74:609-19.
3. Cuddeback SM, Yamaguchi H, Komatsu K, et al. Molecular cloning and characterization of bif-1. J. Biol. Chem. 2001; 276:20559-65.
4. Takahashi Y, Karbowski M, Yamaguchi H, et al. Loss of Bif-1 suppresses Bax/Bak conformational change and mitochondrial apoptosis. Mol. Cell. Biol. 2005; 25:9369-82.

For more detail regarding Peptide please visit here : Custom Peptide Synthesis