Catalog# : 2007
Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A mouse DNase that causes DNA fragmentation was identified recently and designated CAD (for caspase activated deoxyribonuclease). The human homologue of mouse CAD was more recently identified by two groups independently and termed CPAN and DFF40. Human DFF45 and its mouse homologue ICAD are the inhibitors of CPAN/DFF40 and CAD, respectively. Upon cleavage of DFF45/ICAD by activated caspase, DFF40/CAD is released and activated and eventually causes the degradation of DNA in the nuclei. Activation of CAD/DFF40, which causes DNA degradation, is the hallmark of apoptotic cell death.
Additional Names : CAD (I17), DFF40 (I17)
DescriptionLeft: Western blot analysis of CAD in mouse kidney tissue lysate with CAD antibody at 2 µg/ml. Below: Immunohistochemistry of CAD in mouse kidney tissue with CAD antibody at 2 µg/ml.
Source :CAD antibody was raised against a peptide corresponding to 17 amino acids near the center of murine CAD.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : CAD antibody was raised in rabbit.
Immunogen : Human CAD (I17) Peptide (Cat. No. 2007P)
Tested Application(s) : E, WB, IHC
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide : Cat. No. 2007P - CAD Peptide
Long-Term Storage : CAD antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1405 - Mouse Kidney Tissue Lysate
GI Number : 20137695
Accession Number : O54788
Short Description : (I17) Caspase Activated DNase
References
- Enari M, Sakahira H, Yokoyama H, Okawa K, Iwamatsu A, Nagata S. A caspase-activated DNase that degrades DNA during apoptosis, and its inhibitor ICAD. Nature 1998;391:43-50
- Sakahira H, Enari M, Nagata S. Cleavage of CAD inhibitor in CAD activation and DNA degradation during apoptosis. Nature 1998;391:96-99
- Liu X, Li P, Widlak P, Zou H, Luo X, Garrard WT, Wang X The 40-kDa subunit of DNA fragmentation factor induces DNA fragmentation and chromatin condensation during apoptosis. Proc Natl Acad Sci USA 1998;95:8461-6
- Halenbeck R, MacDonald H, Roulston A, Chen TT, Conroy L, Williams LT. CPAN, a human nuclease regulated by the caspase-sensitive inhibitor DFF45. Curr Biol 1998;8:537-40
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