CARD8 Antibody

CARD8 Antibody

Catalog# : 3187

Apoptosis is related to many diseases and development. Cell death signals are transduced by death domain (DD), death effector domain (DED), and caspase recruitment domain (CARD) containing molecules. CARD containing proteins include some caspases, Apaf-1, CARD4, IAPs, RICK, ARC, RAIDD, BCL-10, and ASC. A novel CARD-containing protein was recently identified and designated CARD8. This protein interacts with DRAL, a p53-responsive protein implicated in the induction of apoptosis (1,2), and caspase-1 and its related proteins ICEBERG and pesudo-ICE (3). Although there are conflicting reports on whether CARD8 acts a pro- or anti-apoptotic protein (2,4), it has been suggested that it functions as an adaptor molecule regulating caspase-1 and NF-kappaB activation.

Additional Names : CARD8, Cardinal/TUCAN

Description
Left: Western blot analysis of CARD8 expression in K562 cell lysate with CARD8 antibody at (A) 2 and (B) 4 µg /ml.

Below: Immunocytochemistry of CARD8 in K562 cells with CARD8 antibody at 10 µg/ml.

Other Product Images

Source : CARD8 antibody was raised against a synthetic peptide corresponding to amino acids at the C-terminus of human CARD8.

Purification : Affinity chromatography purified via peptide column

Clonality and Clone : This is a polyclonal antibody.

Host : CARD8 antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.

Immunogen : Human CARD8 / Cardinal / TUCAN Peptide (Cat. No. 3187P)

Application : CARD8 antibody can be used for detection of CARD8 by Western blot at 2 µg/ml.An approximate 45 kDa can be detected.

Tested Application(s) : E, WB, ICC

Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.

Blocking Peptide : Cat. No. 3187P - CARD8 Peptide

Long-Term Storage : CARD8 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Positive Control
1.Cat. No. 1204 - K562 Cell Lysate

Species Reactivity :H

GI Number : 12247742

Accession Number : AAG50014

Short Description : A new CARD containing protein

References
1.Scholl FA, McLoghlin P, Ehler E, et al. DRAL is a p53-responsive gene whose four and a half LIM domain protein product induces apoptosis. J. Cell Biol. 2000; 151:495-506.
2.Stil R, Leonardi A, Formisano L, et al. TUCAN/CARDINAL and DRAL participate in a B activation. FEBS 2002;kcommon pathway for modulation of NF- 521:165-9.
3.Razmara M, Srinivasula SM, Wang L, et al. CARD-8 protein, a new CARD family member that regulates caspase-1 activation and apoptosis. J. Biol. Chem. 2002; 277:13952-8.
4.Pathan N, Marusawa H, Krajewska M, et al. TUCAN, an antiapoptotic caspase-assoicated recruitment domain family protein overexpressed in cancer. J. Biol. Chem. 2001; 276:32220-9.

For more detail regarding Peptide please visit here : Custom Peptide Synthesis