Caspase-5 Antibody

Caspase-5 Antibody

Catalog# : 3457

Caspases are a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens (reviewed in 1). Members of this subfamily include caspase-1, -4, -5, and -12. Activation of these caspases results in the cleavage and activation of proinflammatory cytokines such as IL-1beta and IL-18 (2,3). Caspase-5 can interact with caspase-1; both are constituents of the NALP1 inflammasome, a complex that can trigger the cleavage of pro-IL-1beta (4). Expression of caspase-5 can be regulated by lipopolysaccharide (LPS) and IFN-ϒ (5).

Additional Names : Caspase-5 (IN), ICH-3 , ICE rel III
Description
Left: Western blot analysis of caspase-5 in Ramos cells with caspase-5 antibody at (A) 0.5, (B) 1, and (C) 2 µg/ml.

Below: Immunocytochemistry of caspase-5 in P815 cells with caspase-5 antibody at 2 µg/ml.

Source : Caspase-5 antibody was raised against a 14 amino acid peptide from the center of human Caspase-5.

Purification : Affinity chromatography purified via peptide column

Clonality and Clone : This is a polyclonal antibody.

Host : Caspase-5 antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.

Immunogen : Human Casp-5 / (ICH-3 / ICE rel III (Intermediate Domain) Peptide (Cat. No. 3457P)

Application : Casp-5 antibody can be used for the detection of caspase-5 by Western blot at 0.5 - 1 µg/mlDepending on cell lines or tissues used, other cleavage products may be observed.

Tested Application(s) : E, WB, ICC

Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.

Blocking Peptide : Cat. No. 3457P - Casp-5 Peptide

Long-Term Storage : Caspase-5 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Positive Control
1.Cat. No. 1225 - Ramos Cell Lysate
2.Cat. No. 1286 - P815 Cell Lysate

Species Reactivity :H

GI Number : 212276423

Accession Number : P51878

Short Description : (IN) a caspase in the inflammatory subfamily

References

1.Martinon F and Tschopp J. Inflammatory caspases: linking an intracellular innate immune system to autoinflammatory diseases. Cell 2004; 117:561-74.
2.Kuida K, Lippke JA, Ku G, et al. Altered cytokine export and apoptosis in mice deficient in interleukin-1 beta converting enzyme. Science 1995; 267:2000-3.
3.Gracie JA, Robertson SE, and McInnes IB. Interleukin-18. J. Leukoc. Biol. 2003; 73:213-224.
4.Martinon F, Burns K, and Tschopp J. The inflammasome: a molecular platform triggering activation of the inflammatory caspases and processing of proIL-1. Mol. Cell. 2002; 10:417-26.