Caspase-2 Antibody

Caspase-2 Antibody

Catalog# : 3447

Caspases are a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens (reviewed in 1 and 2). Members of this subfamily include caspase-1, -4, -5, and -12 and can activate proinflammatory cytokines such as IL-1beta and IL-18 (3,4). Although phylogenetically similar to this subfamily, Caspase-2 is thought to be involved in stress-induced apoptosis (5). Caspase-2 has two major isoforms; overexpression on the long form results in apoptosis while that of the short form suppresses cell death (6).

Additional Names : Caspase-2 (NT), ICH-1, Nedd2
Description
Left: Western blot analysis of caspase-2 in Ramos cells with caspase-2 antibody at (A) 0.5, (B) 1, and (C) 2 µg/ml.

Below: Immunocytochemistry of caspase-2 in A-20 cells with caspase-2 antibody at 2 µg/ml.

Other Product Images
Source : Caspase-2 antibody was raised against a 16 amino acid peptide from the amino-terminus of human Caspase-2.

Purification : Affinity chromatography purified via peptide column

Clonality and Clone : This is a polyclonal antibody.

Host : Caspase-2 antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.

Immunogen : Murine Casp-2 / ICH-1 / Nedd2 (N-Terminus) Peptide (Cat. No. 3447P)

Application : Caspase-2 antibody can be used for the detection of caspase-2 by Western blot at 1 µg/ml.Depending on cell lines or tissues used, other cleavage products may be observed.

Tested Application(s) : E, WB, ICC

Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.

Blocking Peptide : Cat. No. 3447P - Casp-2 Peptide

Long-Term Storage : Caspase-2 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Positive Control
1.Cat. No. 1225 - Ramos Cell Lysate
2.Cat. No. 1288 - A20 Cell Lysate

Species Reactivity :H, M

GI Number : 83300977

Accession Number : P42575

Short Description : (NT) a caspase induced by ER stress and DNA damage

References
1.Martinon F and Tschopp J. Inflammatory caspases: linking an intracellular innate immune system to autoinflammatory diseases. Cell 2004; 117:561-74.
2.Zhivotovsky B and Orrenius S. Caspase-2 funtion in response to DNA damage. Biochim. Biophys. Res. Comm. 2005; 331:859-67.
3.Kuida K, Lippke JA, Ku G, et al. Altered cytokine export and apoptosis in mice deficient in interleukin-1 beta converting enzyme. Science 1995; 267:2000-3.
4.Gracie JA, Robertson SE, and McInnes IB. Interleukin-18. J. Leukoc. Biol. 2003; 73:213-224.