Catalog# : 2391
The p53 tumor-suppressor gene integrates numerous signals that control cell life and death. Several novel molecules involved in p53 signaling, including Chk2 , p53R2 (2), p53AIP1 (3), Noxa (4), PIDD (5), and PID/MTA2 (6), were recently discovered. The checkpoint kinase Chk2 is the mammalian homologue of yeast Cds1/Rad53. In response to DNA damage, the checkpoint kinase ATM phosphorylates and activates Chk2, which in turn directly phosphorylates and activates p53 (7,8). Chk2 serves as ATM downstream effector to mediate activation of p53. Chk2 also phosphorylates and activates BRCA1, the product of a tumor suppressor gene that is mutated in breast and ovarian cancer (9).
Additional Names : Chk2 (NT), Chk2
DescriptionLeft: Western blot analysis of Chk2 expression in K562 (A), Jurkat (B), and HL-60 (C) whole cell lysates with Chk2 antibody at 1 µg /ml. .
Below: Immunocytochemistry of Chk2 in Jurkat cells with Chk2 antibody at 1 µg/ml.
Other Product Images
Source : Chk2 antibody was raised against a synthetic peptide corresponding to amino acids near the amino terminus of human Chk2 .Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : Chk2 antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.
Immunogen : Human Chk2 (N-Terminus) Peptide (Cat. No. 2391P)
Application : Chk2 antibody can be used for detection of Chk2 by Western blot at 0.5 to 1 µg/ml.A 60 kDa band can be detected.
Tested Application(s) : E, WB, ICC
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide : Cat. No. 2391P - Chk2 Peptide
Long-Term Storage : Chk2 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
1.Cat. No. 1205 - Jurkat Cell Lysate
2.Cat. No. 1204 - K562 Cell Lysate
Species Reactivity :H, M, R
GI Number : 6005850
Accession Number : NP_009125
Short Description : (NT) A novel check point kinase
References
1.Matsuoka S, Huang M, Elledge SJ. Linkage of ATM to cell cycle regulation by the Chk2 protein kinase. Science. 1998;282:1893-7.
2.Tanaka H, Arakawa H, Yamaguchi T, Shiraishi K, Fukuda S, Matsui K, Takei Y, Nakamura Y. A ribonucleotide reductase gene involved in a p53-dependent cell-cycle checkpoint for DNA damage. Nature. 2000;404:42-9.
3.Oda E, Ohki R, Murasawa H, Nemoto J, Shibue T, Yamashita T, Tokino T, Taniguchi T, Tanaka N. Noxa, a BH3-only member of the Bcl-2 family and candidate mediator of p53-induced apoptosis. Science. 2000;288(5468):1053-8.
4.Oda K, Arakawa H, Tanaka T, Matsuda K, Tanikawa C, Mori T, Nishimori H, Tamai K, Tokino T, Nakamura Y, Taya Y. p53AIP1, a potential mediator of p53-dependent apoptosis, and its regulation by Ser-46-phosphorylated p53. Cell. 2000 Sep 15;102(6):849-62.
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